Vaccinia Virus Vaccine (APSV) in Vaccinia- Naive Subjects: PILOT

INCLUSION CRITERIA: Age: 18-32 (Volunteers over 32 years of age are not included since they usually are not vaccinia-naive.) Children are excluded due to concerns of safety, including autoinoculation. Acceptable medical history by screening evaluation form and brief assessment. Negative urine pregnancy test for women. Negative history of smallpox vaccination. Negative ELISA for HIV or negative Western blot for volunteers who have a positive ELISA and have participated in an HIV vaccine trial. Availability for follow-up for planned duration of the study (6 months). Note: Stage 1 - clinic visit at Day 180+/-15 days, Stage 2 - phone call at Day 180 +/- 15 days. If the volunteer is female, she agrees to use acceptable contraception and not become pregnant for the duration of the study. Willing to sign informed consent. Adequate renal functions as defined as a serum creatinine of 1.5 mg/dL, urine protein < 100 mg/dL or negative proteinuria, and a calculated creatinine clearance > 55 mL/min based on the following formulas: For males ((140 minus age in years) x weight in kg)/72 x serum creatinine). For females: 0.85 x ((140 minus age in years) x weight in kg)/72 x serum creatinine). Note: Renal function is measured to ensure subjects could meet criteria for use of cidofovir if needed. EXCLUSION CRITERIA: History of immunodeficiency. Serology positive for HIV, hepatitis B, or hepatitis C at the screening visit. Known or suspected impairment of immunologic function including, but not limited to, clinically significant liver disease. Diabetes mellitus. Moderate to severe kidney impairment. Malignancy, other than squamous cell or basal cell skin cancer. Autoimmune disease. Use of immunosuppressive medication. Corticosteroid nasal sprays are permissible. Medical or psychiatric condition or occupational responsibilities that preclude subject compliance with the protocol. History of "illegal" injection drug use. Live attenuated vaccines within 60 days of study. Use of experimental agents within 30 days prior to study. Any history of vaccination with any vaccinia vectored or other pox vectored experimental vaccine. Typical vaccinia scar without known history of vaccination. History of smallpox vaccination. Acute febrile illness on the day of vaccination. Pregnant or lactating women. Eczema or any degree or history of eczema. History of exfoliative skin disorders/conditions. Any acute skin disorders of large magnitude, e.g., laceration requiring sutures, burn greater than 2x2 cm. Receipt of blood products or immunoglobulin in the past 6 months. Household contacts/sexual contacts with, or occupational exposure to (other than minimal contact), people with any of the following: pregnancy, < 12 months of age, eczema or history of eczema, previously listed skin disorders/conditions, immunodeficiency disease or use of immunosuppressive medications. Allergies to any component of the vaccines (e.g. polymyxin B sulfate, dihydrostreptomycin sulfate, chlortetracycline hydrochloride, neomycin sulfate). Allergies to any known component of the diluent. Allergies to any known component of VIG, i.e., thimerosal or previous allergic reaction to immunoglobulins. Allergies to cidofovir or probenicid. Blood donation within the past 55 days (stage 1 only).