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Genetic Causes of Male Infertility

Primary Purpose

Male Infertility

Status
Completed
Phase
Locations
Sweden
Study Type
Observational
Intervention
Sponsored by
Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
About
Eligibility
Locations
Outcomes
Full info

About this trial

This is an observational trial for Male Infertility focused on measuring Genotypes, Mutation, Folate, Male Infertility, Nutrition

Eligibility Criteria

20 Years - 45 Years (Adult)MaleDoes not accept healthy volunteers

INCLUSION CRITERIA INFERTILE MEN: Referred to the Scanian Andrology Centre. Age 20-45. Partner age less than 40. Having had regular sexual intercourse without contraception for a year or more without achieving a pregnancy. EXCLUSION CRITERIA INFERTILE MEN: Klinefelters syndrome. Hypogondotropic hypogonadism. Y-chromosome microdeletion or abnormality. Other genetic cause for infertility. Obstructive azoospermia. Partner with salpingitis. Partner with polycystic ovarian syndrome. Partner with disturbance of ovulation. Partner with endometriosis. History of cancer. History of treatment with cytotoxic drugs, irradiation, or sulfasalazopyrine. History of cryptorchidism. History of mumps orchitis. History of vasectomy. INCLUSION CRITERIA FERTILE MEN: Partner attending Lund University prenatal clinic. Age 20-45. Partner age less than 40. Having fathered one or more pregnancies. Having stopped birth control to achieve present pregnancy. Having achieved present pregnancy in less than 12 months of unprotected intercourse. EXCLUSION CRITERIA FERTILE MEN: History of cancer. History of treatment with cytotoxic drugs, irradiation, or sulfasalazopyrine. History of cryptorchidism. History of mumps orchitis. Having sought or partner having sought treatment or investigation for fertility.

Sites / Locations

  • Malmo University Hospital

Outcomes

Primary Outcome Measures

Secondary Outcome Measures

Full Information

First Posted
June 19, 2006
Last Updated
April 4, 2018
Sponsor
Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
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1. Study Identification

Unique Protocol Identification Number
NCT00341120
Brief Title
Genetic Causes of Male Infertility
Official Title
Methylenetetrahydrofolate Reductase C677T Mutation, Other Variant Genotypes, and Male Infertility
Study Type
Observational

2. Study Status

Record Verification Date
August 29, 2012
Overall Recruitment Status
Completed
Study Start Date
January 2, 2003 (undefined)
Primary Completion Date
undefined (undefined)
Study Completion Date
August 29, 2012 (undefined)

3. Sponsor/Collaborators

Name of the Sponsor
Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)

4. Oversight

5. Study Description

Brief Summary
This study is being conducted at the University Hospital of Lund University in Malmo, Sweden, in collaboration with the U.S. National Institute of Child Health and Human Development. The study will try to identify genetic causes of impaired sperm production and male infertility. It will focus on the possible role of the MTHFR and CBS genes, which regulate absorption and metabolism of the vitamin, folate in infertility. If the nutritional intake or metabolism of this vitamin is related to male infertility, then this cause of infertility would be potentially curable. Fertile and infertile men between 20 and 45 years of age may be eligible for this study. Criteria include the following: Fertile men: men whose partners are younger than age 40 and are attending Lund University prenatal clinic; who have fathered one or more pregnancies and who stopped birth control to achieve the present pregnancy; who achieved the present pregnancy in less than 12 months of unprotected intercourse. Infertile men: men referred to the Scandian Andrology Centre whose infertility is unexplained, whose partners are younger than age 40 and who have had regular sexual intercourse without contraception for at least 12 months without achieving a pregnancy. All participants will have the following tests and procedures: Complete a questionnaire providing information about their reproductive and medical history and recent dietary history; Provide blood samples for analysis of red cell folate, plasma folate, plasma homocysteine, plasma B12, and for genetic evaluation; Provide a semen sample for routine analysis, including volume, sperm concentration, sperm motility, and sperm morphology. In addition, infertile men will undergo a physical examination and review of their medical records.
Detailed Description
It is evident that genetic variation plays a substantial role in the etiology of male infertility. Studies of children fathered through intracytoplasmic sperm injection or ICSI have revealed mutations on the AZF region of the Y chromosome linked to male infertility. Mutations of other genes may also be involved. Candidates would include genes for the androgen receptor, follicle-stimulating hormone, and luteinizing hormone, and genes involved in the regulation of spermatogenesis and sperm motility. Mutations in mitochondrial DNA have been linked to poor sperm motility and raise the possibility that some types of male subfertility may be inherited only through the female line. We propose to assess the role of folate/homocysteine status and MTHFR and CBS gene variants in infertile men in Sweden with no known cause for their infertility and whose wives/partners appear to be fertile. We propose to perform the study in Sweden since Sweden, unlike the U.S., at present does not mandate the enrichment of flour or other foodstuffs with folate.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Male Infertility
Keywords
Genotypes, Mutation, Folate, Male Infertility, Nutrition

7. Study Design

Enrollment
400 (Actual)

10. Eligibility

Sex
Male
Minimum Age & Unit of Time
20 Years
Maximum Age & Unit of Time
45 Years
Accepts Healthy Volunteers
No
Eligibility Criteria
INCLUSION CRITERIA INFERTILE MEN: Referred to the Scanian Andrology Centre. Age 20-45. Partner age less than 40. Having had regular sexual intercourse without contraception for a year or more without achieving a pregnancy. EXCLUSION CRITERIA INFERTILE MEN: Klinefelters syndrome. Hypogondotropic hypogonadism. Y-chromosome microdeletion or abnormality. Other genetic cause for infertility. Obstructive azoospermia. Partner with salpingitis. Partner with polycystic ovarian syndrome. Partner with disturbance of ovulation. Partner with endometriosis. History of cancer. History of treatment with cytotoxic drugs, irradiation, or sulfasalazopyrine. History of cryptorchidism. History of mumps orchitis. History of vasectomy. INCLUSION CRITERIA FERTILE MEN: Partner attending Lund University prenatal clinic. Age 20-45. Partner age less than 40. Having fathered one or more pregnancies. Having stopped birth control to achieve present pregnancy. Having achieved present pregnancy in less than 12 months of unprotected intercourse. EXCLUSION CRITERIA FERTILE MEN: History of cancer. History of treatment with cytotoxic drugs, irradiation, or sulfasalazopyrine. History of cryptorchidism. History of mumps orchitis. Having sought or partner having sought treatment or investigation for fertility.
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Richard J Levine, M.D.
Organizational Affiliation
Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
Official's Role
Principal Investigator
Facility Information:
Facility Name
Malmo University Hospital
City
Malmo
Country
Sweden

12. IPD Sharing Statement

Citations:
PubMed Identifier
10802517
Citation
Ma K, Mallidis C, Bhasin S. The role of Y chromosome deletions in male infertility. Eur J Endocrinol. 2000 May;142(5):418-30. doi: 10.1530/eje.0.1420418.
Results Reference
background
PubMed Identifier
11418145
Citation
Ford WC. Biological mechanisms of male infertility. Lancet. 2001 Apr 21;357(9264):1223-4. doi: 10.1016/s0140-6736(00)04452-4. No abstract available.
Results Reference
background
PubMed Identifier
10936107
Citation
Ruiz-Pesini E, Lapena AC, Diez-Sanchez C, Perez-Martos A, Montoya J, Alvarez E, Diaz M, Urries A, Montoro L, Lopez-Perez MJ, Enriquez JA. Human mtDNA haplogroups associated with high or reduced spermatozoa motility. Am J Hum Genet. 2000 Sep;67(3):682-96. doi: 10.1086/303040. Epub 2000 Aug 9.
Results Reference
background

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Genetic Causes of Male Infertility

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