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IPT and Efficacy of Sulphadoxine/Pyrimethamine and Chlorproguanil/Dapsone in 6-59 Month Old Children With Malaria.

Primary Purpose

Malaria

Status
Terminated
Phase
Phase 3
Locations
Tanzania
Study Type
Interventional
Intervention
sulphadoxine/pyrimethamine
Chlorproguanil/dapsone
Sulfadoxine/pyrimethamine
Sponsored by
London School of Hygiene and Tropical Medicine
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional treatment trial for Malaria

Eligibility Criteria

2 Months - 59 Months (Child)All SexesDoes not accept healthy volunteers

For 6-59 month study Inclusion Criteria: Aged 6-59 months Weight of 4.5 Kgs or greater. Not enrolled in IPTi trial Absence of severe malnutrition (defined as a child whose weight-for-height is below 3 standard deviations of less than 70% of the median of WHO reference values, or who has symmetrical edema involving at least the feet) A slide-confirmed infection with P. falciparum only Initial parasite density between 2,000 and 200,000 asexual parasites per microliter. Absence of general danger signs among children < 5 years (see below) Measured axillary temperature ³37.5 °C Ability to attend stipulated follow-up visits Informed consent provided by parent/guardian Absence of history of hypersensitivity reactions to any of the drugs being evaluated Exclusion Criteria: Enrolled in IPTi trial Severe malnutrition (defined as above) No slide confirmed infection with P. falciparum Initial parasite density < 2,000 or > 200,000 asexual parasites per microliter. Presence of general danger signs among children < 5 years (inability to drink or breastfeed; vomiting everything; recent history of convulsions; lethargy or unconsciousness; inability to sit or stand up) or other signs of severe and complicated falciparum malaria according to WHO definitions Measured axillary temperature <37.5 °C Inability to attend stipulated follow-up visits Informed consent not provided by parent/guardian History of hypersensitivity reactions to any of the drugs being evaluated For 2-10 month study Inclusion criteria. Aged 2-10 months Weight of 4.5 Kgs or greater. Not enrolled in IPTi trial Absence of severe malnutrition A slide-confirmed infection with P. falciparum only Any parasite density assessed by research microscopists. Absence of general danger signs among children < 5 years (inability to drink or breastfeed; vomiting everything; recent history of convulsions; lethargy or unconsciousness; inability to sit or stand up) or other signs of severe and complicated falciparum malaria according to WHO definitions Measured axillary temperature < 37.5 °C Ability to attend stipulated follow-up visits Informed consent provided by parent/guardian Absence of history of hypersensitivity reactions to SP. Exclusion criteria are not listed separately in the WHO protocol, as they are the opposite of the inclusion criteria.

Sites / Locations

  • Hale Dispensary

Arms of the Study

Arm 1

Arm 2

Arm 3

Arm Type

Active Comparator

Experimental

Experimental

Arm Label

A

B

C

Arm Description

SP in symptomatic children aged 6-59 months

SP to asymptomatic infected children aged 2-10 months

Chlorproguanil/dapsone in symptomatic 6-59 month old children

Outcomes

Primary Outcome Measures

Clinical /Parasitological Outcomes (WHO 2003)
Early Treatment Failure (ETF)
§ Development of danger signs or severe malaria on Day 1, 2, or 3, in the presence of parasitemia
§ Parasitemia on Day 2 higher than Day 0 count irrespective of axillary temperature
§ Parasitemia on Day 3 with axillary temperature ≥37.5°C
§ Parasitemia on Day 3 ≥ 25% of count on Day 0
Late Clinical Failure (LCF)
§ Development of danger signs or severe malaria from Day 4 to Day 28 in the presence of parasitemia, without previously meeting any of the criteria of ETF
§ Presence of parasitemia and axillary temperature ≥37.5° C on any day from Day 4 to Day 28, without previously meeting any of the criteria of ETF
Late parasitological failure (LPF) -
Presence of parasitemia on Day 14, 21, or 28 and axillary temperature <37.5°C without previously meeting any of the criteria of ETF or LCF (after exclusion of re-infection by PCR for failures at days 14-28)
Adequate Clinical and Parasitological Response (ACPR) -
Absence of parasitemia on Day 28 irrespective of axillary temperature, without previously meeting any of the criteria of ETF, LCF or LPF

Secondary Outcome Measures

Full Information

First Posted
August 3, 2006
Last Updated
January 25, 2017
Sponsor
London School of Hygiene and Tropical Medicine
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1. Study Identification

Unique Protocol Identification Number
NCT00361114
Brief Title
IPT and Efficacy of Sulphadoxine/Pyrimethamine and Chlorproguanil/Dapsone in 6-59 Month Old Children With Malaria.
Official Title
Efficacy of Sulphadoxine/Pyrimethamine and Chlorproguanil/Dapsone in 6-59 Month Old Children With Uncomplicated Malaria and in 2-10 Month Old Asymptomatic Infants.
Study Type
Interventional

2. Study Status

Record Verification Date
January 2017
Overall Recruitment Status
Terminated
Why Stopped
SP arms were stopped due to high levels of treatment failure.CD not available.
Study Start Date
July 2006 (undefined)
Primary Completion Date
August 2007 (Actual)
Study Completion Date
October 2007 (Actual)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
London School of Hygiene and Tropical Medicine

4. Oversight

Data Monitoring Committee
Yes

5. Study Description

Brief Summary
Intermittent Preventive Treatment of malaria in infants is a promising strategy to reduce incidence of clinical malaria in children under the age of 1 year. It is likely to be implemented as a malaria control strategy in Tanzania using sulfadoxine/pyremethamine SP. SP is failing as a first line treatment for clinical episodes of malaria and government policy is driving a change to use Artemesin Combination Therapy (ACT). The main ongoing Kilimanjaro IPTi study is looking at alternatives to SP for use in IPTi. Currently, as there is no evidence for the use of other drugs for IPT, SP will be continued for IPT in pregnancy and in infants. This study proposes to measure the efficacy of SP and chlorproguanil/dapsone (CD), in symptomatic 6- 59 month old children using standard methodology. These are both study drugs in the main IPTi study. This will help us to see how the efficacies of SP and CD in sick children relate to the efficacies for treating asymptomatic children with IPTi. In addition this proposal aims to test the efficacy of SP given to 2-10 month old asymptomatic infants (the target group for IPTi). Evidence suggests that asymptomatic malaria infections with low parasitaemia have a higher cure rate than symptomatic infections with high parasitaemia even when markers of resistance are highly prevalent. This second study aims to quantify this difference and will produce evidence to help policy makers know when drugs used for IPTi should be changed. Both studies will be open label and run concurrently in Hale, Korogwe district near to the main Kilimanjaro IPTi site in Tanzania.
Detailed Description
Introduction and Rational This is part of a multicentre trial looking into antimalarial resistance within a series of Intermittent Preventive Treatment of malaria in Infants (IPTi) studies. IPTi is a promising intervention to reduce malaria in infants in malaria endemic countries. 3 doses of antimalarial drugs are given in the first year of life to asymptomatic infants at the time of 2nd and 3rd Diptheria, Tetanus and Pertusus immunisation and again at 9 months of age at time of measles immunisation. In the current Kilimanjaro IPTi study we are comparing 3 antimalarials (Mefloquine MQ, sulfadoxine/pyremethamine SP and chlorproguanil/dapsone CD) against placebo for IPTi in 2 transmission zones. Two published studies using SP for IPTi have shown different efficacies for this intervention. The first study (Schellenberg et al 2001) showed a 62% reduction in clinical cases of malaria and the second (Chandramohan et al, 2005) showed only a 25% reduction in clinical cases of malaria. The two sites differ in several ways, the first was carried out in an area of moderate to low transmission with a moderate rate of SP resistance in Tanzania and the latter was carried out in an area of intense but seasonal malaria with a low rate of SP resistance in Ghana. The current IPTi study is being carried out in a site of high transmission with expected high rates of SP resistance. Data from a site 100km away indicates SP efficacy to be 55% (Mutabingwa et al, 2001). There is no data on SP or other study drugs at the ongoing study sites. In addition there is evidence (Personal communication Chandramohan 2005) that the outcome (Adequate Parasite Response (APR)) of asymptomatic parasitaemia in infants is better than that in symptomatic 6-59 month old children when using SP. For this reason we plan a novel study examining the efficacy of SP in asymptomatic 2-10 month old infants, the target age group for IPTi. The main objective of this component of the drug resistance study is to understand the relationship between the efficacy of antimalarial drugs used for IPTi and the long term effect of IPTi on incidence of clinical malaria. Schellenberg et al (2005). observed a 36% reduction in the incidence of clinical malaria in the second year of life in the SP IPTi group compared to the placebo group even though the last course of SP IPTi was given at 9 months of age (Schellenberg et al, 2005). This suggests that SP IPTi in an area with moderate malaria transmission and moderate levels of resistance to SP (+/-25%) IPTi may enhance the development of immunity against malaria. In contrast, a study in Ghana in area with very high and seasonal malaria transmission and very low resistance to SP (<10%) showed a 20% increase in the incidence of clinical malaria with high parasite density (>5000 parasites/ml) in SP IPTi group compared to placebo group. The nature of the relationship between drug resistance and the long term effect of IPTi on malaria remains unclear. Parasite clearance rate depends on the efficacy of antimalarial drug and host immunity. Host immunity depends on age - immunity in infants is likely to be lower than the immunity in 1-4 year old children. Parasite clearance rate depends on parasite density, multiplicity of infection, and the type of host response - these factors differ between clinical episodes of malaria and asymptomatic parasitaemia. The interaction between antimalarial drug and host response during a clinical episode of malaria is likely to be different from that during asymptomatic malaria parasitaemia. Although infants with mild illness will not be excluded from IPTi administration at routine EPI clinics, the majority of infants will be asymptomatic when they receive IPTi. Thus results of the standard WHO in vivo drug sensitivity studies conducted in 6-59 month-old symptomatic children are unlikely to be applicable to 2-10 month old mostly asymptomatic infants receiving IPTi. However, there is no empirical evidence to support or refute this assertion. In order to understand the relationship between level of drug resistance and long terms effects of IPTi, the exact level of drug resistance in the IPTi target infants needs to be established and related to IPTi efficacy. Thus a modified WHO in vivo drug resistance study in 2-10 month old asymptomatic infants needs to be conducted as part of the Kilimanjaro IPTi study and a comparison made with the efficacy of SP estimated from a standard WHO 6-59 month WHO in vivo efficacy study. Objectives We therefore propose 2 studies that have the following objectives: Enumerate the drug efficacies of SP and CD in the study area using the standard WHO in vivo drug efficacy methodology in symptomatic children aged 6-59 months, Enumerate the efficacy of SP in asymptomatic 2-10 month olds using a modified WHO in vivo drug efficacy methodology, the target population for IPTi and Determine the difference of efficacy when using SP for the different indications, ie between its use in symptomatic and asymptomatic children. Methods Study 1 is a standard in vivo WHO drug efficacy study looking at parasite positive symptomatic children between the ages of 6 and 59 months treated with SP or CD. They will be followed up after 1,2 3, 7,14,21 and 28 days. At each visit a blood slide and filter paper will be collected. Endpoints are day 28 recrudescence rates. Study 2 is a modified in vivo drug efficacy study in the age group directly affected by IPTi, namely 2-10 months of age. Asymptomatic children will be followed up at days 1, 2, 3,7,14, 21 and 28 only. Endpoint is day 28 recrudescence rate. Blood slide and filter paper will only be taken on day 7, 14 and 28 unless the child has a fever or has reported fever in the last 48 hours. Sample size and study subjects Table 1 Study Arm Expected (assumed) rate of adequate parasitological response by day 28 (APR) Acceptable 95% confidence limit (precision) Sample Size needed based on expected APR and acceptable precision Total sample size (corrected for assumed 10% loss to follow-up and 10% nonadherence) 6-59 month symptomatic children SP 60% +/-6% 292* 330 CD 805 +/-7.5% 131 145 2-10 month asymptomatic children SP 75% +/-7.5% 146* 161 *SP study numbers are calculated to detect a 15% difference of APR by day 28 between symptomatic 6-59 month old children and asymptomatic 2-10 month old children with 80% power at the 5% significance level using a ratio of 2 symptomatic cases to 1 asymptomatic case. Children attending a single health dispensary (Hale dispensary, Korogwe District,Tanga Region, Tanzania) aged between 6-59 months with a fever or history of fever within the preceding 48 hours will be asked to take part. If they fit the entry criteria (see attached protocol) and consent, they will be enrolled in the standard WHO in vivo efficacy study. Asymptomatic children aged between 2 and 10 months attending the MCH clinic for immunisations and weighing will be invited to take part in the 2-10 month efficacy study and those eligible for the study and consenting will be enrolled into the study. Study Site The project will take place in Hale Dispensary, a government dispensary offering primary care and MCH services to a population of more than 8,000, with an average of 30 new births a month. Hale is situated in Korogwe district,Tanga region, northeastern Tanzania and is lowland with high malaria transmission. It is less than 10 km from the IPTi study site. Ethical considerations It is expected that first line antimalarial drug policy will change in November 2006 from SP to lumefantrine/arthemeter. After this date it will not be ethical to continue the 6-59 month study in symptomatic children. However for the asymptomatic children it will be ethical to continue as SP use continues in asymptomatic women for IPTp and if adopted as a strategy for Tanzania, SP will be used for IPTi. Therefore we plan to carry out the SP efficacy in 6-59 month study in symptomatic children as soon as possible and if the national policy changes before completion of the study we will have to halt it. The 2-10 month old asymptomatic study will run concurrently until completion. In the study the second line drug will be lumefantrine/arthemeter for all children should treatment fail. Clinical safety of subjects is paramount. We will encourage mothers to bring their children for review during the follow up period when ever the child is sick. We have budgeted for a transport allowance for the mothers to help them make the journey when necessary. We will ensure 24 hour access to a member of the study team at all time. This person will be able to call on medical staff covering all clinical trial activities in Korogwe District Hospital for advice and possible emergency transfer to the hospital and assessment. An emergency vehicle is on standby 24 hours a day.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Malaria

7. Study Design

Primary Purpose
Treatment
Study Phase
Phase 3
Interventional Study Model
Single Group Assignment
Masking
None (Open Label)
Allocation
Non-Randomized
Enrollment
112 (Actual)

8. Arms, Groups, and Interventions

Arm Title
A
Arm Type
Active Comparator
Arm Description
SP in symptomatic children aged 6-59 months
Arm Title
B
Arm Type
Experimental
Arm Description
SP to asymptomatic infected children aged 2-10 months
Arm Title
C
Arm Type
Experimental
Arm Description
Chlorproguanil/dapsone in symptomatic 6-59 month old children
Intervention Type
Drug
Intervention Name(s)
sulphadoxine/pyrimethamine
Other Intervention Name(s)
Fansidar
Intervention Description
Sulfadoxine/pyremethamine (SP) given as a single dose per manufacturers recommendation (tablets 500mg sulphadoxine and 15mg pyremethamine; child's body weight 5-10Kgs ½ tablet, 11-20Kgs 1 tablet, 21-30 Kgs 1 ½ tablets)
Intervention Type
Drug
Intervention Name(s)
Chlorproguanil/dapsone
Other Intervention Name(s)
Lapdap
Intervention Description
Chlorproguanil/ dapsone (CD) given daily for 3 days per manufacturers recommendations (tablets chlorproguanil 15mg/ dapsone 18.75 mg; 5-7.9Kgs 1 tablet, 8-11.9 Kg 1 ½ tablets, 12-15.9 2 tablets, 16-20.9 Kgs 2 ½ tablets, 21- 40Kg 5 tablets).
Intervention Type
Drug
Intervention Name(s)
Sulfadoxine/pyrimethamine
Other Intervention Name(s)
Fansidar
Intervention Description
Sulfadoxine/pyremethamine (SP) given as a single dose per manufacturers recommendation (tablets 500mg sulphadoxine and 15mg pyremethamine; child's body weight 5-10Kgs ½ tablet, 11-20Kgs 1 tablet, 21-30 Kgs 1 ½ tablets)
Primary Outcome Measure Information:
Title
Clinical /Parasitological Outcomes (WHO 2003)
Time Frame
Day 14 and 28
Title
Early Treatment Failure (ETF)
Time Frame
3 days
Title
§ Development of danger signs or severe malaria on Day 1, 2, or 3, in the presence of parasitemia
Time Frame
3 Days
Title
§ Parasitemia on Day 2 higher than Day 0 count irrespective of axillary temperature
Time Frame
2 nd day
Title
§ Parasitemia on Day 3 with axillary temperature ≥37.5°C
Time Frame
3 rd day
Title
§ Parasitemia on Day 3 ≥ 25% of count on Day 0
Time Frame
3 rd Day
Title
Late Clinical Failure (LCF)
Time Frame
After Day 3 to day 28
Title
§ Development of danger signs or severe malaria from Day 4 to Day 28 in the presence of parasitemia, without previously meeting any of the criteria of ETF
Time Frame
Day 4 - 28
Title
§ Presence of parasitemia and axillary temperature ≥37.5° C on any day from Day 4 to Day 28, without previously meeting any of the criteria of ETF
Time Frame
Day 4 to Day 28
Title
Late parasitological failure (LPF) -
Time Frame
Day 4 - 28
Title
Presence of parasitemia on Day 14, 21, or 28 and axillary temperature <37.5°C without previously meeting any of the criteria of ETF or LCF (after exclusion of re-infection by PCR for failures at days 14-28)
Time Frame
Day 14, 21, or 28
Title
Adequate Clinical and Parasitological Response (ACPR) -
Time Frame
Day 28
Title
Absence of parasitemia on Day 28 irrespective of axillary temperature, without previously meeting any of the criteria of ETF, LCF or LPF
Time Frame
Day 28

10. Eligibility

Sex
All
Minimum Age & Unit of Time
2 Months
Maximum Age & Unit of Time
59 Months
Accepts Healthy Volunteers
No
Eligibility Criteria
For 6-59 month study Inclusion Criteria: Aged 6-59 months Weight of 4.5 Kgs or greater. Not enrolled in IPTi trial Absence of severe malnutrition (defined as a child whose weight-for-height is below 3 standard deviations of less than 70% of the median of WHO reference values, or who has symmetrical edema involving at least the feet) A slide-confirmed infection with P. falciparum only Initial parasite density between 2,000 and 200,000 asexual parasites per microliter. Absence of general danger signs among children < 5 years (see below) Measured axillary temperature ³37.5 °C Ability to attend stipulated follow-up visits Informed consent provided by parent/guardian Absence of history of hypersensitivity reactions to any of the drugs being evaluated Exclusion Criteria: Enrolled in IPTi trial Severe malnutrition (defined as above) No slide confirmed infection with P. falciparum Initial parasite density < 2,000 or > 200,000 asexual parasites per microliter. Presence of general danger signs among children < 5 years (inability to drink or breastfeed; vomiting everything; recent history of convulsions; lethargy or unconsciousness; inability to sit or stand up) or other signs of severe and complicated falciparum malaria according to WHO definitions Measured axillary temperature <37.5 °C Inability to attend stipulated follow-up visits Informed consent not provided by parent/guardian History of hypersensitivity reactions to any of the drugs being evaluated For 2-10 month study Inclusion criteria. Aged 2-10 months Weight of 4.5 Kgs or greater. Not enrolled in IPTi trial Absence of severe malnutrition A slide-confirmed infection with P. falciparum only Any parasite density assessed by research microscopists. Absence of general danger signs among children < 5 years (inability to drink or breastfeed; vomiting everything; recent history of convulsions; lethargy or unconsciousness; inability to sit or stand up) or other signs of severe and complicated falciparum malaria according to WHO definitions Measured axillary temperature < 37.5 °C Ability to attend stipulated follow-up visits Informed consent provided by parent/guardian Absence of history of hypersensitivity reactions to SP. Exclusion criteria are not listed separately in the WHO protocol, as they are the opposite of the inclusion criteria.
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Roly D Gosling, MD
Organizational Affiliation
London School of Hygiene and Tropical Medicine
Official's Role
Principal Investigator
First Name & Middle Initial & Last Name & Degree
Samwel Gesase, MD
Organizational Affiliation
National Institute of Medical Research, Tanzania
Official's Role
Principal Investigator
First Name & Middle Initial & Last Name & Degree
Jaqueline Mosha, MD
Organizational Affiliation
Kilimanjaro Christian Medical College, Tanzania
Official's Role
Principal Investigator
Facility Information:
Facility Name
Hale Dispensary
City
Korogwe
State/Province
Tanga Region
Country
Tanzania

12. IPD Sharing Statement

Citations:
PubMed Identifier
19238219
Citation
Gesase S, Gosling RD, Hashim R, Ord R, Naidoo I, Madebe R, Mosha JF, Joho A, Mandia V, Mrema H, Mapunda E, Savael Z, Lemnge M, Mosha FW, Greenwood B, Roper C, Chandramohan D. High resistance of Plasmodium falciparum to sulphadoxine/pyrimethamine in northern Tanzania and the emergence of dhps resistance mutation at Codon 581. PLoS One. 2009;4(2):e4569. doi: 10.1371/journal.pone.0004569. Epub 2009 Feb 24.
Results Reference
derived

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IPT and Efficacy of Sulphadoxine/Pyrimethamine and Chlorproguanil/Dapsone in 6-59 Month Old Children With Malaria.

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