Profiling of Original Cellular and Humoral Biomarkers of Type 1 Diabetes (Lymphoscreen)

The "Lymphoscreen" study aims to characterize precisely (phenotypes/cytokines/functions) CD8+ T cell responses in type 1 Diabetes to identify biomarkers of the disease. Such markers are needed for refine type 1 Diabetes diagnosis/prognostic, and to design new therapeutic approaches targeting autoreactive CD8+ T cells. An original approach using DNA immunization of humanized mice allowed us to identify relevant CD8 epitopes derived from GAD65 and IA-2 beta cell autoantigens. The aims are: (i) identifying exhaustively epitopes recognized by autoreactive CD8+ T lymphocytes in type 1 Diabetes and following islet or pancreas graft in humans; (ii) identifying pathogenic CD8+ T cell patterns or profiles related to type 1 Diabetes pathogenesis and evolution; (iii) correlating CD8+ autoreactive T cell responses and autoantibody responses to new cellular (such as CD4+ T cells or peripheral cell miRNA) or humoral markers of the disease (such as serum miRNA).

Identification and characterization of new CD8+ T lymphocytes related to type 1 diabetes and its evolution (2009-2012)

Identification and characterization of new profiles of humoral and cellular markers (including T cell reactivity and miRNA) related to type 1 diabetes (2010-2014).

Inclusion criteria: at least, 50 patients with "recent" type 1 diabetes, 30 patients with long-term type 1 diabetes, 10 patients with Latent Autoimmune Diabetes, 10 subjects with a risk for diabetes, 20 type 1 diabetic patients with pancreatic graft or Langerhans islet graft. 50 healthy subjects paired to HLA class I and to the age Those subjects have to respect the following criteria : Age from 7 to 70 -Caucasian Affiliated to a national insurance scheme Written informed consent obtained For children, written informed consent is required from the two parents. Non-inclusion criteria : Age strictly inferior to 7 or strictly superior to 70 years old Pregnancy Secondary diabetes No written informed consent