Back to results

Ultra Low Dose Interleukin-2 in Healthy Volunteers

Primary Purpose

Hematologic Diseases

Status

Completed

Phase

Phase 1

Locations

United States

Study Type

Interventional

Intervention

Interleukin-2 (100,00 IU/m(2) SQ)

About this trial

This is an interventional treatment trial for Hematologic Diseases focused on measuring Interleukin-2, IL-2, Stem Cells, Regulatory T Cells (T regs), Natural Killer Cells (NK cells), Healthy Volunteer, HV

Eligibility Criteria

18 Years - 99 Years (Adult, Older Adult)All SexesDoes not accept healthy volunteers

INCLUSION CRITERIA:
Healthy volunteers confirmed by brief history and physical examination and blood work through the CHI screening protocol
Males or females ages 18 years or older

EXCLUSION CRITERIA:

History of inflammatory or autoimmune disease
History of inflammatory or autoimmune disease in first degree relatives
History of malignancies
Recipients of hematopoietic stem cell transplant or solid organ transplant
History of seizures
Positive HIV, hepatitis B surface antigen or hepatitis C antibody
History or clinical signs of cardiac disease including coronary artery disease, cardiac arrhythmia, congestive heart failure
History or clinical signs of of pulmonary disease including obstructive lung disease and asthma
Active infections requiring systemic antibiotic therapy or anti-viral therapy
History of systemic fungal or mycobacterial infections
Use of immune modifying medications, i.e.- non steroidal anti-inflammatory drugs (aspirin, ibuprofen, naproxen, celecoxib, ketrolac), steroid (prednisone, dexamethasone, hydrocortisone), chemotherapy (cisplatin, dacarbazine, interferon alpha, tamoxifen)
Persons who are alcoholic or abusers of illicit drugs
Female subjects who are or may be pregnant or lactating
Psychiatric diagnoses or symptoms, to include hypomania, bipolar disorder, major depression, or dysthymia
Abnormal or marginal peripheral blood count that in the opinion of the PI will cause Hemoglobin and Hematocrit levels to drop as a result of participation in this study
Liver function tests above the normal laboratory reference range
Renal function test above the normal laboratory reference range
Contraindication to interleukin-2 (i.e. hypersensitivity to IL- 2 products, active coronary artery disease, patients with organ allograft)
Recent recipient of any type of vaccination (i.e. rotavirus vaccine, BCG, influenza virus vaccine, rubella virus vaccine, mumps virus vaccine, measles vaccine, poliovirus vaccine, smallpox vaccine, typhoid vaccine, varicella virus vaccine, yellow fever vaccine) in the 4 weeks preceding and during active study participation
Body mass index greater than 35
Inability to comprehend the investigational nature of the study or provide informed consent
Diabetes mellitus or fasting blood glucose of > 100 mg/dL.
Any drugs or supplements that interfere with blood clotting such as Vit.E, NSAIDS, Warfarin (Coumadin), ticlopidine (Ticlid), clopidogrel (Plavix)
Use of iodinated contrast media in the 4 weeks preceding and during active study participation.

Sites / Locations

National Institutes of Health Clinical Center, 9000 Rockville Pike

Arms of the Study

Arm 1

Arm 2

Arm Type

Experimental

Arm Label

Arm Description

100,000 international units/m2 SQ daily for 5 days

200,000 international units/m2 SQ daily for 5 days

Outcomes

Primary Outcome Measures

Safety and tolerability of ultra low dose IL-2. TRSAE rate of no more than 5% would allow us to proceed with future studies.

To determine the safety and the tolerability of ultra low dose (physiologic dose) interleukin-2 in healthy volunteers

Secondary Outcome Measures

Human immune response (immunome) to ultra low dose IL-2 based on research lab assessments

Full Information

NCT ID

NCT01445561

First Posted

September 30, 2011

Last Updated

March 13, 2020

Sponsor

National Heart, Lung, and Blood Institute (NHLBI)

1. Study Identification

Unique Protocol Identification Number

NCT01445561

Brief Title

Ultra Low Dose Interleukin-2 in Healthy Volunteers

Official Title

The Safety and the Tolerability of Ultra Low Dose Interleukin-2 in Healthy Volunteers

Study Type

Interventional

2. Study Status

Record Verification Date

March 2020

Overall Recruitment Status

Completed

Study Start Date

September 28, 2011 (Actual)

Primary Completion Date

July 15, 2013 (Actual)

Study Completion Date

July 15, 2013 (Actual)

3. Sponsor/Collaborators

Responsible Party, by Official Title

Sponsor

Name of the Sponsor

National Heart, Lung, and Blood Institute (NHLBI)

4. Oversight

Studies a U.S. FDA-regulated Drug Product

Studies a U.S. FDA-regulated Device Product

5. Study Description

Brief Summary

Background: - Interleukin-2 is a drug that can help stimulate the body s response to inflammation. High dose interleukin-2 has been used to treat different types of cancer and immune system disorders. However, it can cause frequent and often serious side effects at the doses currently used for treatment. Very low dose interleukin-2 (700 folds less than regular dose) was previously tested in cancer patients and stem cell transplant recipients. The study observed important immune changes and minimal side effects in those patients. Researchers want to test the healthy immune system's responses to very low doses of interleukin-2 to better understand how the drug works. Objectives: - To study the effects of very low doses of interleukin-2 on healthy volunteers. Eligibility: - Healthy volunteers at least 18 years of age. Design: Participants will be screened with a medical history and physical exam. They will also have blood and urine samples. Participants will receive one of two possible very low doses of interleukin-2 every day for 5 days. Blood samples will be taken twice before the first dose, 1 day after the first dose, and before the next three doses. Followup blood samples will be taken on Days 7, 14, and 28 after the first dose.

Detailed Description

Interleukin 2 (IL-2, aldesleukin) was discovered as a T cell growth factor more than 30 years ago. IL-2 was the first human cytokine used therapeutically. IL-2 induces antigen specific T cells, and two important lymphocyte subsets: regulatory T cells (T-regs) and natural killer cells (NK) cells. T-regs have a critical role in self-tolerance and pathogenesis of autoimmune disease or graft versus host disease (GVHD), and they have been extensively studied in solid tumors, hematologic malignancies, viral hepatitis, and HIV infections. NK cells have a unique role in bridging innate and adaptive immunity. NK cells facilitate hematopoietic stem cell (HSC) engraftment reduce GVHD and increase graft-versus-leukemia (GVL) effects. NK cells have important roles on pathogenesis of malignancies, autoimmune disease and AIDS. Conventional dose IL-2 treatment promotes marked expansion of regulatory T cells, and NK cells but is associated with significant side effects. However, much lower doses of interleukin-2 (0.5- 1MIU/m2/day) which lack significant side effects, also induce expansion of T regs and NK cells. These observations suggest that ultra low dose IL-2 would be safe and appropriate to give to hematopoietic stem cell donors. The quality of the transplant would be improved because the higher dose of T-regs would reduce the risk of GVHD while the higher NK cells would augment the GVL effect. The aim of this study is to evaluate the safety and the tolerability of ultra low dose IL-2 in healthy volunteer for preferential expansion of T-regs and NK cells with a view to extending ultra low dose IL-2 administration to stem cell donors. We anticipate that this study will provide valuable information on the biology of IL-2 on the human immunome applicable to various human disease conditions, including cancer, immunodeficiency disease, autoimmune disease, and hematopoietic stem cell transplantation. The proposed IL-2 dose is 2-3 logs lower than the manufacturer s recommended dose. We therefore expect that the dose used in this protocol will be well tolerated. Nevertheless, because there is little information on the tolerability and safety of IL-2 at these ultra low doses, we have structured this study as a safety protocol with stopping rules for unacceptable side effects. This is important because we hope to use the safety data generated to justify a future protocol giving ultra low dose IL-2 to stem cell donors.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study

Hematologic Diseases

Keywords

Interleukin-2, IL-2, Stem Cells, Regulatory T Cells (T regs), Natural Killer Cells (NK cells), Healthy Volunteer, HV

7. Study Design

Primary Purpose

Treatment

Study Phase

Phase 1

Interventional Study Model

Parallel Assignment

Masking

None (Open Label)

Allocation

Non-Randomized

Enrollment

22 (Actual)

8. Arms, Groups, and Interventions

Arm Title

Arm Type

Experimental

Arm Description

100,000 international units/m2 SQ daily for 5 days

Arm Title

Arm Type

Experimental

Arm Description

200,000 international units/m2 SQ daily for 5 days

Intervention Type

Drug

Intervention Name(s)

Interleukin-2 (100,00 IU/m(2) SQ)

Intervention Description

100,000 International Units/m2 SQ daily for 5 days

Primary Outcome Measure Information:

Title

Safety and tolerability of ultra low dose IL-2. TRSAE rate of no more than 5% would allow us to proceed with future studies.

Description

To determine the safety and the tolerability of ultra low dose (physiologic dose) interleukin-2 in healthy volunteers

Time Frame

28 days

Secondary Outcome Measure Information:

Title

Human immune response (immunome) to ultra low dose IL-2 based on research lab assessments

Time Frame

28 days

10. Eligibility

Sex

All

Minimum Age & Unit of Time

18 Years

Maximum Age & Unit of Time

99 Years

Accepts Healthy Volunteers

Eligibility Criteria

INCLUSION CRITERIA: Healthy volunteers confirmed by brief history and physical examination and blood work through the CHI screening protocol Males or females ages 18 years or older EXCLUSION CRITERIA: History of inflammatory or autoimmune disease History of inflammatory or autoimmune disease in first degree relatives History of malignancies Recipients of hematopoietic stem cell transplant or solid organ transplant History of seizures Positive HIV, hepatitis B surface antigen or hepatitis C antibody History or clinical signs of cardiac disease including coronary artery disease, cardiac arrhythmia, congestive heart failure History or clinical signs of of pulmonary disease including obstructive lung disease and asthma Active infections requiring systemic antibiotic therapy or anti-viral therapy History of systemic fungal or mycobacterial infections Use of immune modifying medications, i.e.- non steroidal anti-inflammatory drugs (aspirin, ibuprofen, naproxen, celecoxib, ketrolac), steroid (prednisone, dexamethasone, hydrocortisone), chemotherapy (cisplatin, dacarbazine, interferon alpha, tamoxifen) Persons who are alcoholic or abusers of illicit drugs Female subjects who are or may be pregnant or lactating Psychiatric diagnoses or symptoms, to include hypomania, bipolar disorder, major depression, or dysthymia Abnormal or marginal peripheral blood count that in the opinion of the PI will cause Hemoglobin and Hematocrit levels to drop as a result of participation in this study Liver function tests above the normal laboratory reference range Renal function test above the normal laboratory reference range Contraindication to interleukin-2 (i.e. hypersensitivity to IL- 2 products, active coronary artery disease, patients with organ allograft) Recent recipient of any type of vaccination (i.e. rotavirus vaccine, BCG, influenza virus vaccine, rubella virus vaccine, mumps virus vaccine, measles vaccine, poliovirus vaccine, smallpox vaccine, typhoid vaccine, varicella virus vaccine, yellow fever vaccine) in the 4 weeks preceding and during active study participation Body mass index greater than 35 Inability to comprehend the investigational nature of the study or provide informed consent Diabetes mellitus or fasting blood glucose of > 100 mg/dL. Any drugs or supplements that interfere with blood clotting such as Vit.E, NSAIDS, Warfarin (Coumadin), ticlopidine (Ticlid), clopidogrel (Plavix) Use of iodinated contrast media in the 4 weeks preceding and during active study participation.

Overall Study Officials:

First Name & Middle Initial & Last Name & Degree

Chen Zhao, M.D.

Organizational Affiliation

National Heart, Lung, and Blood Institute (NHLBI)

Official's Role

Principal Investigator

Facility Information:

Facility Name

National Institutes of Health Clinical Center, 9000 Rockville Pike

City

Bethesda

State/Province

Maryland

ZIP/Postal Code

20892

Country

United States

12. IPD Sharing Statement

Citations:

PubMed Identifier

3131876

Citation

Smith KA. Interleukin-2: inception, impact, and implications. Science. 1988 May 27;240(4856):1169-76. doi: 10.1126/science.3131876.

Results Reference

background

PubMed Identifier

181845

Citation

Morgan DA, Ruscetti FW, Gallo R. Selective in vitro growth of T lymphocytes from normal human bone marrows. Science. 1976 Sep 10;193(4257):1007-8. doi: 10.1126/science.181845.

Results Reference

background

PubMed Identifier

2280612

Citation

Macdonald D, Jiang YZ, Gordon AA, Mahendra P, Oskam R, Palmer PA, Franks CR, Barrett AJ. Recombinant interleukin 2 for acute myeloid leukaemia in first complete remission: a pilot study. Leuk Res. 1990;14(11-12):967-73. doi: 10.1016/0145-2126(90)90109-m.

Results Reference

background

Links:

URL

https://clinicalstudies.info.nih.gov/cgi/detail.cgi?B_2011-H-0268.html

Description

NIH Clinical Center Detailed Web Page

Learn more about this trial

Ultra Low Dose Interleukin-2 in Healthy Volunteers

We'll reach out to this number within 24 hrs