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Macrophages Effect on Chemoresistance

Primary Purpose

the Focus of the Study is Macrophage-induced Chemoresistance in Pancreatic Carcinoma Cells During Chemotherapy.

Status
Unknown status
Phase
Not Applicable
Locations
Study Type
Interventional
Intervention
taking blood samples from healthy people
Sponsored by
Rambam Health Care Campus
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional basic science trial for the Focus of the Study is Macrophage-induced Chemoresistance in Pancreatic Carcinoma Cells During Chemotherapy. focused on measuring macrophages, adenocarcinoma, chemoresistance

Eligibility Criteria

18 Years - 40 Years (Adult)All SexesAccepts Healthy Volunteers

Inclusion Criteria:

  • healthy people

Exclusion Criteria:

  • refusal to participate

Sites / Locations

    Arms of the Study

    Arm 1

    Arm Type

    Other

    Arm Label

    irrelevant

    Arm Description

    Outcomes

    Primary Outcome Measures

    proliferation and migration of PANC1 cell line in the tested groups.
    the macrophages will be isolated from the blood we'll get from the healthy volunteers. the outcome measure is related to the basic research and not clinical so there's no symptom/outcome related to the participants.

    Secondary Outcome Measures

    To determine whether direct cell-to-cell interaction or soluble factors participate in MIC.

    Full Information

    First Posted
    August 5, 2013
    Last Updated
    August 9, 2013
    Sponsor
    Rambam Health Care Campus
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    1. Study Identification

    Unique Protocol Identification Number
    NCT01921699
    Brief Title
    Macrophages Effect on Chemoresistance
    Official Title
    Impact of Macrophages on De-Novo Chemoresistance of Adenocarcinoma Cells
    Study Type
    Interventional

    2. Study Status

    Record Verification Date
    August 2013
    Overall Recruitment Status
    Unknown status
    Study Start Date
    August 2013 (undefined)
    Primary Completion Date
    September 2013 (Anticipated)
    Study Completion Date
    undefined (undefined)

    3. Sponsor/Collaborators

    Responsible Party, by Official Title
    Principal Investigator
    Name of the Sponsor
    Rambam Health Care Campus

    4. Oversight

    5. Study Description

    Brief Summary
    Macrophages are derived from monocytes recruited to the tumor site and stimulated by specific chemokines secreted by tumor cells. These tumor associated macrophages (TAMs) have been postulated as being involved in the progression of cancer. Based on our preliminary findings and on published data we hypothesized that macrophage-induced chemoresistance (MIC) can promote survival of pancreatic carcinoma cells during chemotherapy. The overall goal of this study is to evaluate the mechanism of MIC in an in-vitro model of Pancreatic ductal adenocarcinoma (PDA). methods: The human PDA cell line Panc1 will be grown in suitable conditions. Macrophages will be produced by incubating mononuclear cells from the blood of healthy donors in medium with M-CSF for 7 days. TAMs will be generated by culturing these macrophages with tumor-culture conditioning medium (TCCM) of PDA Cells for an additional 72 hours. Human pancreatic cells (PANC1) will be treated with gemcitabine following exposure to macrophages CM. Cell proliferation will be quantified by light microscopy and by an XTT Cell Proliferation Assay Kit.

    6. Conditions and Keywords

    Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
    the Focus of the Study is Macrophage-induced Chemoresistance in Pancreatic Carcinoma Cells During Chemotherapy.
    Keywords
    macrophages, adenocarcinoma, chemoresistance

    7. Study Design

    Primary Purpose
    Basic Science
    Study Phase
    Not Applicable
    Interventional Study Model
    Single Group Assignment
    Masking
    None (Open Label)
    Allocation
    N/A
    Enrollment
    2 (Anticipated)

    8. Arms, Groups, and Interventions

    Arm Title
    irrelevant
    Arm Type
    Other
    Intervention Type
    Procedure
    Intervention Name(s)
    taking blood samples from healthy people
    Primary Outcome Measure Information:
    Title
    proliferation and migration of PANC1 cell line in the tested groups.
    Description
    the macrophages will be isolated from the blood we'll get from the healthy volunteers. the outcome measure is related to the basic research and not clinical so there's no symptom/outcome related to the participants.
    Time Frame
    one day to get the blood samples, and a year after that to complete the basic research
    Secondary Outcome Measure Information:
    Title
    To determine whether direct cell-to-cell interaction or soluble factors participate in MIC.
    Time Frame
    a year from obtaining the blood samples

    10. Eligibility

    Sex
    All
    Minimum Age & Unit of Time
    18 Years
    Maximum Age & Unit of Time
    40 Years
    Accepts Healthy Volunteers
    Accepts Healthy Volunteers
    Eligibility Criteria
    Inclusion Criteria: healthy people Exclusion Criteria: refusal to participate

    12. IPD Sharing Statement

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    Macrophages Effect on Chemoresistance

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