Macrophages Effect on Chemoresistance
Primary Purpose
the Focus of the Study is Macrophage-induced Chemoresistance in Pancreatic Carcinoma Cells During Chemotherapy.
Status
Unknown status
Phase
Not Applicable
Locations
Study Type
Interventional
Intervention
taking blood samples from healthy people
Sponsored by
About this trial
This is an interventional basic science trial for the Focus of the Study is Macrophage-induced Chemoresistance in Pancreatic Carcinoma Cells During Chemotherapy. focused on measuring macrophages, adenocarcinoma, chemoresistance
Eligibility Criteria
Inclusion Criteria:
- healthy people
Exclusion Criteria:
- refusal to participate
Sites / Locations
Arms of the Study
Arm 1
Arm Type
Other
Arm Label
irrelevant
Arm Description
Outcomes
Primary Outcome Measures
proliferation and migration of PANC1 cell line in the tested groups.
the macrophages will be isolated from the blood we'll get from the healthy volunteers. the outcome measure is related to the basic research and not clinical so there's no symptom/outcome related to the participants.
Secondary Outcome Measures
To determine whether direct cell-to-cell interaction or soluble factors participate in MIC.
Full Information
NCT ID
NCT01921699
First Posted
August 5, 2013
Last Updated
August 9, 2013
Sponsor
Rambam Health Care Campus
1. Study Identification
Unique Protocol Identification Number
NCT01921699
Brief Title
Macrophages Effect on Chemoresistance
Official Title
Impact of Macrophages on De-Novo Chemoresistance of Adenocarcinoma Cells
Study Type
Interventional
2. Study Status
Record Verification Date
August 2013
Overall Recruitment Status
Unknown status
Study Start Date
August 2013 (undefined)
Primary Completion Date
September 2013 (Anticipated)
Study Completion Date
undefined (undefined)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
Rambam Health Care Campus
4. Oversight
5. Study Description
Brief Summary
Macrophages are derived from monocytes recruited to the tumor site and stimulated by specific chemokines secreted by tumor cells. These tumor associated macrophages (TAMs) have been postulated as being involved in the progression of cancer.
Based on our preliminary findings and on published data we hypothesized that macrophage-induced chemoresistance (MIC) can promote survival of pancreatic carcinoma cells during chemotherapy.
The overall goal of this study is to evaluate the mechanism of MIC in an in-vitro model of Pancreatic ductal adenocarcinoma (PDA).
methods:
The human PDA cell line Panc1 will be grown in suitable conditions.
Macrophages will be produced by incubating mononuclear cells from the blood of healthy donors in medium with M-CSF for 7 days.
TAMs will be generated by culturing these macrophages with tumor-culture conditioning medium (TCCM) of PDA Cells for an additional 72 hours.
Human pancreatic cells (PANC1) will be treated with gemcitabine following exposure to macrophages CM.
Cell proliferation will be quantified by light microscopy and by an XTT Cell Proliferation Assay Kit.
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
the Focus of the Study is Macrophage-induced Chemoresistance in Pancreatic Carcinoma Cells During Chemotherapy.
Keywords
macrophages, adenocarcinoma, chemoresistance
7. Study Design
Primary Purpose
Basic Science
Study Phase
Not Applicable
Interventional Study Model
Single Group Assignment
Masking
None (Open Label)
Allocation
N/A
Enrollment
2 (Anticipated)
8. Arms, Groups, and Interventions
Arm Title
irrelevant
Arm Type
Other
Intervention Type
Procedure
Intervention Name(s)
taking blood samples from healthy people
Primary Outcome Measure Information:
Title
proliferation and migration of PANC1 cell line in the tested groups.
Description
the macrophages will be isolated from the blood we'll get from the healthy volunteers. the outcome measure is related to the basic research and not clinical so there's no symptom/outcome related to the participants.
Time Frame
one day to get the blood samples, and a year after that to complete the basic research
Secondary Outcome Measure Information:
Title
To determine whether direct cell-to-cell interaction or soluble factors participate in MIC.
Time Frame
a year from obtaining the blood samples
10. Eligibility
Sex
All
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
40 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria:
healthy people
Exclusion Criteria:
refusal to participate
12. IPD Sharing Statement
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Macrophages Effect on Chemoresistance
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