Persistence of Varicella Immunity in Hospital Employees Receiving Varicella Vaccine
Primary Purpose
Persistence of Varicella Immunity
Status
Withdrawn
Phase
Phase 4
Locations
United States
Study Type
Interventional
Intervention
Varicella vaccine
Sponsored by
About this trial
This is an interventional health services research trial for Persistence of Varicella Immunity
Eligibility Criteria
Inclusion Criteria:
- Age 18-80
Group 1: * Evaluated in Occupational Health Clinic (OHC) for employment from 2004-2009 and found to be seronegative for varicella.
- Received at least one varicella vaccination to meet employment criteria at least 5 year prior to enrollment.
- Subject is afebrile (<100.4F oral) on day of vaccination.
- Group 2: * Evaluated in OHC for employment from 2004 -2009 and found to be seropositive for varicella.
Exclusion Criteria:
- Immune suppressed or receiving immune suppressive treatment
- Pregnant, breastfeeding or plan to become pregnant within 6 weeks after receiving varicella vaccine.
- Receiving antiviral medications active against varicella virus within 3 days before varicella vaccination until 10 days after varicella vaccination.
- Receipt of any vaccine within 2 weeks before enrollment or expected receipt within 6 weeks after vaccination.
- Receipt of any immunoglobulins or blood products within 5 months prior to study enrollment or expected receipt prior to last visit.
- History of hypersensitivity reaction to any vaccine component (including gelatin), or an anaphylactic/anaphylactoid reaction to neomycin.
- History of chickenpox or shingles since pre-employment serology testing.
- Receipt of any additional varicella or zoster vaccine since vaccination under hospital policy and procedure guidelines.
- Currently participating in any other studies with an investigational compound within 30 days of signing the consent and throughout participation in the study.
Sites / Locations
- Children's Hospital Colorado
Arms of the Study
Arm 1
Arm 2
Arm Type
No Intervention
Active Comparator
Arm Label
Sero-positive for varicella at least 5 years prior
Varicella vaccine
Arm Description
No treatment
One dose varicella vaccine (Varivax).
Outcomes
Primary Outcome Measures
Determine the proportion of employees who 5 years previously received varicella vaccine at the time of employment who no longer have varicella-specific antibody.
VZV-specific antibody measured by a sensitive gp-ELISA method at CDC
Secondary Outcome Measures
Determine the avidity (strength with which the vaccine induced antibody binds to the varicella antigen) of varicella-specific antibody in vaccinated employees.
Avidity in these employees will be compared to the avidity of antibody in employees who did hot require vaccination because of natural immunity.
Full Information
NCT ID
NCT02026089
First Posted
December 23, 2013
Last Updated
February 4, 2021
Sponsor
University of Colorado, Denver
Collaborators
Centers for Disease Control and Prevention
1. Study Identification
Unique Protocol Identification Number
NCT02026089
Brief Title
Persistence of Varicella Immunity in Hospital Employees Receiving Varicella Vaccine
Official Title
Persistence of Varicella Immunity in Hospital Employees Receiving Varicella Vaccine
Study Type
Interventional
2. Study Status
Record Verification Date
February 2021
Overall Recruitment Status
Withdrawn
Why Stopped
Unforeseen reduction of recruitment resource.
Study Start Date
December 2021 (Anticipated)
Primary Completion Date
June 2022 (Anticipated)
Study Completion Date
June 2022 (Anticipated)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Sponsor
Name of the Sponsor
University of Colorado, Denver
Collaborators
Centers for Disease Control and Prevention
4. Oversight
Studies a U.S. FDA-regulated Drug Product
Yes
Studies a U.S. FDA-regulated Device Product
No
Product Manufactured in and Exported from the U.S.
Yes
Data Monitoring Committee
No
5. Study Description
Brief Summary
The purpose of the study is to learn more abou how the immune system responds to the chickenpox vaccine in adults who had never had chickenpox as a child and were then vaccinated as an adult. The study aims to learn more about the strength and duration of the protection provided by the vaccine in adults, and to observe the immune response to an additional dose of chickenpox vaccine in adults who had received the vaccine at least 5 years earlier. This is especially relevant to workers in a hospital who may be exposed to chickenpox.
Detailed Description
The population will be employees who were previously evaluated in the pre-employment phase by the Occupational Health Clinic (OHC) of Colorado Children's Hospital (CHC) from 2004 until 2009 and were found to be seronegative for varicella antibody at that time and were thus vaccinated per CHC protocol. Additionally, a matching number of comparator subjects who were positive for varicella antibody at the pre-employment phase will be selected from the same pool (2004-2009).
Subject participation will last up to 6 months. Subjects will be enrolled into one of two study groups:
Group 1 consisting of 50 subjects who tested seronegative for varicella antibody and received varicella vaccine to meet employment criteria at least 5 years previously; and
Group 2 consisting of 50 subjects who had natural varicella infection and thus tested seropositive for varicella antibody at least 5 years previously.
Group 1 subjects will receive 1 additional dose (lifetime total will be 2 or 3) of varicella vaccine (Varivax) and participate in a total of 4 study visits over a period of 6 months. Before receiving the dose of varicella vaccine at Day 1, Group 1 subjects will be given the CDC Vaccine Information Sheet for varicella vaccine. Study staff will review this with the subject to assure understanding prior to administering vaccine. Vaccination will occur after eligibility criteria have been confirmed and completion of a urine pregnancy test -with a negative result - on females of childbearing potential. Following administration of the varicella vaccine, the subject will be observed in the clinic for at least 15 minutes for safety. Blood (60 ml per sample) will be collected from all Group 1 subjects on Day 1 immediately prior to vaccination, and at Day 7(±2), Day 30(±7) and Day 180(±14) in order to measure VZV-specific antibody, antibody avidity, and cell-mediated immunity by the methods indicated above. Subjects receiving a third lifetime dose of Varivax will be asked to complete a diary for 5 days after vaccination to track anticipated and unanticipated reactions to the vaccine.
Group 2 subjects, the comparator group, will receive no vaccine and will only participate in the first visit for a blood draw. Blood (60 ml) will be collected from Group 2 participants only at Day 1. Subject information to be recorded includes age; gender; varicella vaccine history at the OHC; age vaccinated (calculate interval since vaccination); the number of doses (1 vs 2) received; and any exposure to varicella or HZ (including involvement in any CHC outbreak work-up).
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Persistence of Varicella Immunity
7. Study Design
Primary Purpose
Health Services Research
Study Phase
Phase 4
Interventional Study Model
Parallel Assignment
Masking
None (Open Label)
Allocation
Non-Randomized
Enrollment
0 (Actual)
8. Arms, Groups, and Interventions
Arm Title
Sero-positive for varicella at least 5 years prior
Arm Type
No Intervention
Arm Description
No treatment
Arm Title
Varicella vaccine
Arm Type
Active Comparator
Arm Description
One dose varicella vaccine (Varivax).
Intervention Type
Biological
Intervention Name(s)
Varicella vaccine
Other Intervention Name(s)
Varivax
Intervention Description
Varivax 0.5ml subcutaneous injection
Primary Outcome Measure Information:
Title
Determine the proportion of employees who 5 years previously received varicella vaccine at the time of employment who no longer have varicella-specific antibody.
Description
VZV-specific antibody measured by a sensitive gp-ELISA method at CDC
Time Frame
Outcome measure data obtained at visit 1; assessed in the aggregate 3-6 months after completion of study.
Secondary Outcome Measure Information:
Title
Determine the avidity (strength with which the vaccine induced antibody binds to the varicella antigen) of varicella-specific antibody in vaccinated employees.
Description
Avidity in these employees will be compared to the avidity of antibody in employees who did hot require vaccination because of natural immunity.
Time Frame
Outcome measure data obtained at visit 1; assessed in the aggregate 3-6 months after completion of study.
Other Pre-specified Outcome Measures:
Title
Determine the varicella-specific T-cell and B-cell responses in vaccinees.
Description
Responses will be compared to those present in employees who did not require vaccination because of natural immunity.
Time Frame
At 7 and 30 days post-vaccination
10. Eligibility
Sex
All
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
80 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria:
Age 18-80
Group 1: * Evaluated in Occupational Health Clinic (OHC) for employment from 2004-2009 and found to be seronegative for varicella.
Received at least one varicella vaccination to meet employment criteria at least 5 year prior to enrollment.
Subject is afebrile (<100.4F oral) on day of vaccination.
Group 2: * Evaluated in OHC for employment from 2004 -2009 and found to be seropositive for varicella.
Exclusion Criteria:
Immune suppressed or receiving immune suppressive treatment
Pregnant, breastfeeding or plan to become pregnant within 6 weeks after receiving varicella vaccine.
Receiving antiviral medications active against varicella virus within 3 days before varicella vaccination until 10 days after varicella vaccination.
Receipt of any vaccine within 2 weeks before enrollment or expected receipt within 6 weeks after vaccination.
Receipt of any immunoglobulins or blood products within 5 months prior to study enrollment or expected receipt prior to last visit.
History of hypersensitivity reaction to any vaccine component (including gelatin), or an anaphylactic/anaphylactoid reaction to neomycin.
History of chickenpox or shingles since pre-employment serology testing.
Receipt of any additional varicella or zoster vaccine since vaccination under hospital policy and procedure guidelines.
Currently participating in any other studies with an investigational compound within 30 days of signing the consent and throughout participation in the study.
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Myron J Levin, MD
Organizational Affiliation
University of Colorado School of Medicine
Official's Role
Principal Investigator
Facility Information:
Facility Name
Children's Hospital Colorado
City
Aurora
State/Province
Colorado
ZIP/Postal Code
80045
Country
United States
12. IPD Sharing Statement
Plan to Share IPD
No
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Persistence of Varicella Immunity in Hospital Employees Receiving Varicella Vaccine
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