Molecular Evaluation of Endometrium After Endometrial Injury
Endometrial Injury
About this trial
This is an interventional treatment trial for Endometrial Injury
Eligibility Criteria
Inclusion Criteria:
- < 40 years old
- Patients had history of 3 failed consecutive cycles of IVF / ICSI.
- Patients were good responders in prior ovulation induction cycle.
- There were at least two embryos with grade A in each embryo transfer cycle.
- Normal uterus in Hysterosalpingography (HSG) or ultrasound or hysteroscopy scans.
- The minimum thickness of the endometrium is 7 mm in injection day.
Exclusion Criteria:
- Submucosal myoma.
- Intramural and sub serousal myoma greater than 5 cm.
- Endometrioma equal to or greater than 3 cm or Hydrosalpinx.
- The number of available embryos is less than 2 in the current cycle.
- Patients with endometrial tuberculosis and persons who are treated for tuberculosis.
- Patients with any specific drug consumption.
- Patients with a history of thyroid disease, diabetes and other endocrine disorders.
- Lost to follow up or sampling.
- Impossibility of prepare endometrial sampling because of severe pain or probability infection.
Sites / Locations
- Royan Institute
Arms of the Study
Arm 1
Arm 2
Experimental
No Intervention
Endometrial injury
Control
In the intervention group, endometrial sampling is obtained twice by Pipelle [one in the follicular phase (during 8-9 or 11- 13 day in the beginning of buserelin cycle) and the last in the luteal phase (during 19-21 or 20-23 day) preceding the embryo transfer cycle preceding the embryo transfer cycle]. Blood samples (5- 10 cc) are taken in the both groups twice (one on the 9-8 or 11- 13 day and 19-21 or 20-23 day preceding the embryo transfer cycle).
In the control group endometrial sampling will be done only in the luteal phase of the cycle preceding the embryo transfer cycle. Blood samples (5- 10 cc) are taken twice (one on the 9-8 or 11- 13 day and 19-21 or 20-23 day preceding the embryo transfer cycle).