Diagnostic Accuracy of Polymerase Chain Reaction for Mycobacterium Tuberculosis Using EBUS-TBNA Samples
Lymphadenopathy, Tuberculosis, Sarcoidosis
About this trial
This is an interventional diagnostic trial for Lymphadenopathy focused on measuring Bronchoscopy, Ultrasonography, Tuberculous lymphadenitis, Polymerase chain reaction, Sensitivity and specificity
Eligibility Criteria
Inclusion Criteria:
- Patients with isolated intrathoracic lymphadenopathy
Exclusion Criteria:
- Suspicion of primary lung cancer on CT or PET scan
- Suspicion of pulmonary tuberculosis on CT or PET scan
- Suspicion of sarcoidosis of lung parenchyma on CT or PET scan
Sites / Locations
- Pusan National University HospitalRecruiting
Arms of the Study
Arm 1
Arm 2
Arm 3
Experimental
Experimental
Experimental
Nested PCR for formalin-fixed tissues
Nested PCR for fresh tissues
Real-time PCR for fresh tissues
In all patients, nested polymerase chain reaction for Mycobacterium tuberculosis is performed with formalin-fixed paraffin-embedded specimens obtained by endobronchial ultrasound-guided transbronchial needle aspiration.
In all patients, nested polymerase chain reaction for Mycobacterium tuberculosis is performed with specimens in sterile saline obtained by endobronchial ultrasound-guided transbronchial needle aspiration.
In all patients, real-time polymerase chain reaction for Mycobacterium tuberculosis is performed with specimens in sterile saline obtained by endobronchial ultrasound-guided transbronchial needle aspiration.