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Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging (S48)

Primary Purpose

Flaccid Muscle Tone

Status
Completed
Phase
Not Applicable
Locations
United States
Study Type
Interventional
Intervention
muscle biopsy
Sponsored by
Purdue University
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional other trial for Flaccid Muscle Tone

Eligibility Criteria

20 Years - 29 Years (Adult)MaleAccepts Healthy Volunteers

-Inclusion Criteria:

  • men 20-29 yrs
  • BMI between 20 and 34.9
  • Fasting Glucose <110 mg/dL
  • Blood Pressure <160/100mm Hg
  • Non-Diabetic
  • LDL-Cholesterol <160mg/dL
  • Triacylglycerol <400 mg/dL
  • Makers of kidney, liver, and heart functions within 10% of clinical normalcy
  • <1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months

Exclusion Criteria:

  • men <20or>29 yrs

    • BMI between <20 and >34.9
    • Fasting Glucose >110 mg/dL
    • Blood Pressure >160/100mm Hg
    • Non-Diabetic
    • LDL-Cholesterol >160mg/dL
    • Triacylglycerol >400 mg/dL

    • Pre-existing kidney or liver conditions

      -->1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months

    • Currently taking prescribed blood thinners

Sites / Locations

  • Purdue University - Stone Hall 700 W State St

Arms of the Study

Arm 1

Arm Type

Experimental

Arm Label

muscle biopsy

Arm Description

a muscle sample will be taken from the mid-thigh (Vastus Lateralis) and analyzed

Outcomes

Primary Outcome Measures

Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging
this study is to obtain human muscle samples for cell culturing which will be exposed to a novel real-time imaging technique in order to elucidate factors that influence muscle satellite cell growth.

Secondary Outcome Measures

Full Information

First Posted
February 10, 2016
Last Updated
June 19, 2017
Sponsor
Purdue University
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1. Study Identification

Unique Protocol Identification Number
NCT02685319
Brief Title
Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging
Acronym
S48
Official Title
Mapping Human Muscle Satellite Cell Fate By Real-Time
Study Type
Interventional

2. Study Status

Record Verification Date
June 2017
Overall Recruitment Status
Completed
Study Start Date
February 2016 (undefined)
Primary Completion Date
May 2016 (Actual)
Study Completion Date
June 2016 (Actual)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
Purdue University

4. Oversight

Data Monitoring Committee
No

5. Study Description

Brief Summary
Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging
Detailed Description
The aim of this study is to obtain human muscle samples for cell culturing to analyze muscle cell development in real-time to uncover factors that influence growth.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Flaccid Muscle Tone

7. Study Design

Primary Purpose
Other
Study Phase
Not Applicable
Interventional Study Model
Single Group Assignment
Masking
None (Open Label)
Allocation
N/A
Enrollment
7 (Actual)

8. Arms, Groups, and Interventions

Arm Title
muscle biopsy
Arm Type
Experimental
Arm Description
a muscle sample will be taken from the mid-thigh (Vastus Lateralis) and analyzed
Intervention Type
Procedure
Intervention Name(s)
muscle biopsy
Intervention Description
a muscle sample will be taken from the mid-thigh (Vastus Lateralis) and analyzed
Primary Outcome Measure Information:
Title
Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging
Description
this study is to obtain human muscle samples for cell culturing which will be exposed to a novel real-time imaging technique in order to elucidate factors that influence muscle satellite cell growth.
Time Frame
<10 hrs

10. Eligibility

Sex
Male
Minimum Age & Unit of Time
20 Years
Maximum Age & Unit of Time
29 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
-Inclusion Criteria: men 20-29 yrs BMI between 20 and 34.9 Fasting Glucose <110 mg/dL Blood Pressure <160/100mm Hg Non-Diabetic LDL-Cholesterol <160mg/dL Triacylglycerol <400 mg/dL Makers of kidney, liver, and heart functions within 10% of clinical normalcy <1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months Exclusion Criteria: men <20or>29 yrs BMI between <20 and >34.9 Fasting Glucose >110 mg/dL Blood Pressure >160/100mm Hg Non-Diabetic LDL-Cholesterol >160mg/dL Triacylglycerol >400 mg/dL Pre-existing kidney or liver conditions -->1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months Currently taking prescribed blood thinners
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Wayne W Campbell, Ph.D.
Organizational Affiliation
Purdue University
Official's Role
Principal Investigator
Facility Information:
Facility Name
Purdue University - Stone Hall 700 W State St
City
West Lafayette
State/Province
Indiana
ZIP/Postal Code
47907
Country
United States

12. IPD Sharing Statement

Plan to Share IPD
No

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Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging

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