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Trial to Evaluate Safety and Immunogenicity of an Ebola Zaire Vaccine in Healthy Adults

Primary Purpose

Ebola Virus Disease

Status
Completed
Phase
Phase 1
Locations
United States
Study Type
Interventional
Intervention
Ebola Vaccine - low dose
Ebola Vaccine - mid dose
Ebola Vaccine - high dose
Placebo
Sponsored by
Auro Vaccines LLC
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional prevention trial for Ebola Virus Disease focused on measuring Ebola, VSV, Vesicular Stomatitis Virus, Vaccine, Zaire

Eligibility Criteria

18 Years - 60 Years (Adult)All SexesAccepts Healthy Volunteers

INCLUSION CRITERIA:

Subjects who have provided written informed consent and an authorization for disclosure of protected health information must meet the following criteria:

  1. Healthy adult men or women, 18 to 60 (inclusive) years of age.
  2. Have provided written informed consent prior to screening procedures.
  3. Free of clinically significant health problems, as determined by pertinent medical history, physical examination without significant findings in the 28 days prior to enrollment, and clinical judgment of the Investigator.
  4. Agrees not to have, or plan to have, non-study vaccines within 60 days after receiving the initial study vaccine, unless medically indicated (i.e., tetanus, rabies vaccine).
  5. Agrees not to have contact with ruminant animals or other hoofed animals such as horses, pigs and cows 7 days after each vaccination.
  6. Available, able, and willing to participate for all study visits and procedures through Day 182 (Week 26).
  7. Be willing to minimize blood and body fluid exposure of others for 7 days after vaccination by:

    • using effective barrier prophylaxis, such as latex condoms, during penetrative sexual intercourse;
    • avoiding the sharing of needles, razors, or toothbrushes; and
    • avoiding open mouth kissing.
  8. Body mass index (BMI) less than 40 kg/m2.
  9. Laboratory criteria without clinically significant findings within 28 days prior to enrollment:

    • hemoglobin ≥11.5 g/dL for women and ≥13.5 g/dL for men.
    • white blood cell count ≥3500 cells/mm3.
    • differential either within institutional normal range or accompanied by site physician approval;
    • platelets within normal limits;
    • alanine aminotransferase (ALT) or aspartate aminotransferase (AST) and alkaline phosphatase within upper limit of normal range or as approved by the Investigator; and
    • serum creatinine within upper limit of normal range or as approved by the Investigator.
  10. Negative for Food and Drug Administration (FDA) approved HIV blood test.
  11. Negative hepatitis B surface antigen (HbsAg).
  12. Negative antibody to hepatitis C virus (antiHCV).
  13. Normal urinalysis defined as negative or trace glucose, protein, and blood (non-menstruating females) by dipstick.
  14. Negative urine pregnancy test for women of childbearing potential.
  15. Non-pregnant, non-lactating females must meet one of the following criteria: no reproductive potential because of menopause (one year without menses) or because of a hysterectomy, bilateral oophorectomy, or tubal ligation; or subject agrees to be heterosexually inactive at least 21 days prior to enrollment and throughout the duration of the study; or agrees to consistently practice contraception at least 21 days prior to enrollment and throughout the duration of the study by one of the following methods: abstinence, condoms (male or female with or without a spermicide), diaphragm or cervical cap with spermicide, intrauterine device, contraceptive pills or patch, Norplant, Depo-Provera or other FDA approved contraceptive method, or male partner has previously undergone a vasectomy as declared in medical history.

EXCLUSION CRITERIA:

Any subject who meets any of the following criteria will not qualify for entry into the study:

  1. History of prior infection with a filovirus or prior participation in a filovirus vaccine trial.
  2. History of prior infection with VSV or receipt of a VSV vectored vaccine.
  3. Has traveled to an area where the World Health Organization (WHO) has declared as an Ebola outbreak zone.
  4. Healthcare worker who has direct contact with patients (nurse, physician, dentist, emergency medical technician, dental hygienist).
  5. Has a household contact (HHC) who is immunodeficient, on immunosuppressive medications, HIV positive, pregnant or breast-feeding, or has an unstable medical condition.
  6. Breast-feeding, or is a childcare worker, or HHC, who has direct contact with children, 5 years of age or younger.
  7. Direct hands-on job preparing food in the food industry.
  8. History of employment in an industry involved in contact with ruminant animals, other hoofed animals such as pigs and horses, veterinary sciences, or other potential exposure to VSV.
  9. History of employment or activity that involves potential contact with filoviruses.
  10. History of severe local or systemic reactions to any vaccination or a history of severe allergic reactions.
  11. Known allergy to any rVSVN4CT1 vectored vaccine component.
  12. Receipt of investigational product (IP) up to 30 days prior to randomization or ongoing participation in another clinical trial except observational studies.
  13. Receipt of licensed non-live or live vaccines within 30 days prior to planned study immunization.
  14. Ability to observe possible local reactions at the eligible injection sites (deltoid region) is, in the opinion of the Investigator, unacceptably obscured due to a physical condition or permanent body art.
  15. Acute or chronic, clinically significant dermatologic, psychiatric, hematologic, pulmonary, cardiovascular, or hepatic or renal functional abnormality as determined by the Investigator based on medical history, physical examination, and/or laboratory screening test. This would include a known autoimmune arthritis, hemoglobinopathy or coagulation abnormality as judged by the Investigator.
  16. Leukopenia due to a clinical or pathological process, unless leukopenia is directly attributable to a transient process (e.g., acute viral infection) and resolution has been documented at time of enrollment.
  17. Any screening or baseline laboratory test which in the opinion of the Investigator, is considered clinically significant.
  18. Any cytotoxic therapy in the previous 5 years.
  19. Diabetes mellitus (type I or II), with the exception of previous gestational diabetes.
  20. Any chronic or active neurologic disorder, such as migraines (including silent), seizures or epilepsy (excluding a single febrile seizure as a child as judged by the Investigator).
  21. Have a known history of Guillain Barré Syndrome (GBS).
  22. Have an active malignancy or history of metastatic or hematologic malignancy.
  23. Suspected or known alcohol and/or illicit drug abuse within the past 5 years per the judgment of the Investigator.
  24. Moderate or severe illness and/or fever >100.4 °F within 1 week prior to vaccination (subjects can be rescheduled).
  25. Administration of immunoglobulin G (IgGs) and/or any blood products within the 120 days preceding study entry or planned administration during the study period.
  26. History of blood donation within 60 days of enrollment or plans to donate within the study period.
  27. Administration of chronic (defined as more than 14 days) immunosuppressants or other immune modifying drugs within 6 months of study entry: for corticosteroids, this includes chronic oral >14 days or intraarticular steroids in the past 6 months (intranasal and topical are allowed).
  28. Major surgery or hospitalization planned during the period of study participation.
  29. Research staff or the immediate family of research staff directly involved with the clinical study.
  30. Any other significant finding that in the opinion of the Investigator that would increase the risk of the individual having an adverse outcome from participating in this study.

Sites / Locations

  • Optimal Research

Arms of the Study

Arm 1

Arm 2

Arm 3

Arm 4

Arm Type

Experimental

Experimental

Experimental

Placebo Comparator

Arm Label

Ebola Vaccine - low dose

Ebola Vaccine - mid dose

Ebola Vaccine - high dose

Placebo

Arm Description

Low Dose Zaire Ebola Vaccine

Mid Dose Zaire Ebola Vaccine

High Dose Zaire Ebola Vaccine

Placebo

Outcomes

Primary Outcome Measures

Incidence of Treatment Emergent Adverse Events (Safety and Tolerability)
To establish the maximum safe and tolerated dose of a monovalent Ebola Zaire vaccine, as determined by local and systemic reactogenicity signs and symptoms, laboratory measures of safety, and adverse and serious adverse experiences.

Secondary Outcome Measures

Full Information

First Posted
March 9, 2016
Last Updated
April 3, 2017
Sponsor
Auro Vaccines LLC
Collaborators
Accelovance, United States Department of Defense
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1. Study Identification

Unique Protocol Identification Number
NCT02718469
Brief Title
Trial to Evaluate Safety and Immunogenicity of an Ebola Zaire Vaccine in Healthy Adults
Official Title
A Phase 1 Clinical Trial to Evaluate the Safety and Immunogenicity of a Monovalent Ebola Zaire Vaccine (rVSVN4CT1-EBOVGP1) Delivered by Intramuscular Injection in Healthy Adult Subjects
Study Type
Interventional

2. Study Status

Record Verification Date
April 2017
Overall Recruitment Status
Completed
Study Start Date
December 22, 2015 (Actual)
Primary Completion Date
September 15, 2016 (Actual)
Study Completion Date
September 15, 2016 (Actual)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Sponsor
Name of the Sponsor
Auro Vaccines LLC
Collaborators
Accelovance, United States Department of Defense

4. Oversight

Data Monitoring Committee
Yes

5. Study Description

Brief Summary
The purpose of this study is to assess the safety profile of the Zaire Ebola vaccine and the strength of the immune response.
Detailed Description
Ebola Zaire is a filovirus that has caused devastating epidemics of hemorrhagic fever in South Africa. Research is underway to create a safe and effective vaccine to protect against Ebola disease, especially for the military and health care workers. Promising animal studies with this vaccine indicate safety and immunogenicity, and the vaccine platform used to deliver the Ebola protein antigen has been successful in creating a safe and protective immune response in people. Note that only one Ebola protein is used in this vaccine; since the entire intact Ebola virus is required for infection, it is impossible to get Ebola disease from this vaccine. The study targets enrollment of 39 healthy adults. These participants are divided into 3 groups that will be administered one of three dose levels of the vaccine (low, medium, high). The study participants will receive two doses of vaccine: one on day 1 and the second on day 28 (1 month). Three participants at each dose level will act as controls and receive a placebo instead of the active vaccine. A total of 13 visits to the clinic are required over a period of 26 weeks. The total study is expected to take 9 months.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Ebola Virus Disease
Keywords
Ebola, VSV, Vesicular Stomatitis Virus, Vaccine, Zaire

7. Study Design

Primary Purpose
Prevention
Study Phase
Phase 1
Interventional Study Model
Parallel Assignment
Masking
ParticipantCare ProviderInvestigator
Allocation
Randomized
Enrollment
38 (Actual)

8. Arms, Groups, and Interventions

Arm Title
Ebola Vaccine - low dose
Arm Type
Experimental
Arm Description
Low Dose Zaire Ebola Vaccine
Arm Title
Ebola Vaccine - mid dose
Arm Type
Experimental
Arm Description
Mid Dose Zaire Ebola Vaccine
Arm Title
Ebola Vaccine - high dose
Arm Type
Experimental
Arm Description
High Dose Zaire Ebola Vaccine
Arm Title
Placebo
Arm Type
Placebo Comparator
Arm Description
Placebo
Intervention Type
Biological
Intervention Name(s)
Ebola Vaccine - low dose
Other Intervention Name(s)
Ebola Zaire Vaccine (2.5 x 10^4 pfu)
Intervention Description
2 x 0.5ml vaccine will be given as intramuscular injections on each of day 1 and day 28
Intervention Type
Biological
Intervention Name(s)
Ebola Vaccine - mid dose
Other Intervention Name(s)
Ebola Zaire Vaccine (2.5 x 10^5 pfu)
Intervention Description
2 x 0.5ml vaccine will be given as intramuscular injections on each of day 1 and day 28
Intervention Type
Biological
Intervention Name(s)
Ebola Vaccine - high dose
Other Intervention Name(s)
High Dose Ebola Zaire Vaccine (2.0 x 10^6 pfu)
Intervention Description
2 x 1.0ml vaccine will be given as intramuscular injections on each of day 1 and day 28
Intervention Type
Biological
Intervention Name(s)
Placebo
Intervention Description
2 x 0.5ml or 2 x 1.0ml placebo will be given as intramuscular injections on each of day 1 and day 28 depending on cohort
Primary Outcome Measure Information:
Title
Incidence of Treatment Emergent Adverse Events (Safety and Tolerability)
Description
To establish the maximum safe and tolerated dose of a monovalent Ebola Zaire vaccine, as determined by local and systemic reactogenicity signs and symptoms, laboratory measures of safety, and adverse and serious adverse experiences.
Time Frame
9 months

10. Eligibility

Sex
All
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
60 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
INCLUSION CRITERIA: Subjects who have provided written informed consent and an authorization for disclosure of protected health information must meet the following criteria: Healthy adult men or women, 18 to 60 (inclusive) years of age. Have provided written informed consent prior to screening procedures. Free of clinically significant health problems, as determined by pertinent medical history, physical examination without significant findings in the 28 days prior to enrollment, and clinical judgment of the Investigator. Agrees not to have, or plan to have, non-study vaccines within 60 days after receiving the initial study vaccine, unless medically indicated (i.e., tetanus, rabies vaccine). Agrees not to have contact with ruminant animals or other hoofed animals such as horses, pigs and cows 7 days after each vaccination. Available, able, and willing to participate for all study visits and procedures through Day 182 (Week 26). Be willing to minimize blood and body fluid exposure of others for 7 days after vaccination by: using effective barrier prophylaxis, such as latex condoms, during penetrative sexual intercourse; avoiding the sharing of needles, razors, or toothbrushes; and avoiding open mouth kissing. Body mass index (BMI) less than 40 kg/m2. Laboratory criteria without clinically significant findings within 28 days prior to enrollment: hemoglobin ≥11.5 g/dL for women and ≥13.5 g/dL for men. white blood cell count ≥3500 cells/mm3. differential either within institutional normal range or accompanied by site physician approval; platelets within normal limits; alanine aminotransferase (ALT) or aspartate aminotransferase (AST) and alkaline phosphatase within upper limit of normal range or as approved by the Investigator; and serum creatinine within upper limit of normal range or as approved by the Investigator. Negative for Food and Drug Administration (FDA) approved HIV blood test. Negative hepatitis B surface antigen (HbsAg). Negative antibody to hepatitis C virus (antiHCV). Normal urinalysis defined as negative or trace glucose, protein, and blood (non-menstruating females) by dipstick. Negative urine pregnancy test for women of childbearing potential. Non-pregnant, non-lactating females must meet one of the following criteria: no reproductive potential because of menopause (one year without menses) or because of a hysterectomy, bilateral oophorectomy, or tubal ligation; or subject agrees to be heterosexually inactive at least 21 days prior to enrollment and throughout the duration of the study; or agrees to consistently practice contraception at least 21 days prior to enrollment and throughout the duration of the study by one of the following methods: abstinence, condoms (male or female with or without a spermicide), diaphragm or cervical cap with spermicide, intrauterine device, contraceptive pills or patch, Norplant, Depo-Provera or other FDA approved contraceptive method, or male partner has previously undergone a vasectomy as declared in medical history. EXCLUSION CRITERIA: Any subject who meets any of the following criteria will not qualify for entry into the study: History of prior infection with a filovirus or prior participation in a filovirus vaccine trial. History of prior infection with VSV or receipt of a VSV vectored vaccine. Has traveled to an area where the World Health Organization (WHO) has declared as an Ebola outbreak zone. Healthcare worker who has direct contact with patients (nurse, physician, dentist, emergency medical technician, dental hygienist). Has a household contact (HHC) who is immunodeficient, on immunosuppressive medications, HIV positive, pregnant or breast-feeding, or has an unstable medical condition. Breast-feeding, or is a childcare worker, or HHC, who has direct contact with children, 5 years of age or younger. Direct hands-on job preparing food in the food industry. History of employment in an industry involved in contact with ruminant animals, other hoofed animals such as pigs and horses, veterinary sciences, or other potential exposure to VSV. History of employment or activity that involves potential contact with filoviruses. History of severe local or systemic reactions to any vaccination or a history of severe allergic reactions. Known allergy to any rVSVN4CT1 vectored vaccine component. Receipt of investigational product (IP) up to 30 days prior to randomization or ongoing participation in another clinical trial except observational studies. Receipt of licensed non-live or live vaccines within 30 days prior to planned study immunization. Ability to observe possible local reactions at the eligible injection sites (deltoid region) is, in the opinion of the Investigator, unacceptably obscured due to a physical condition or permanent body art. Acute or chronic, clinically significant dermatologic, psychiatric, hematologic, pulmonary, cardiovascular, or hepatic or renal functional abnormality as determined by the Investigator based on medical history, physical examination, and/or laboratory screening test. This would include a known autoimmune arthritis, hemoglobinopathy or coagulation abnormality as judged by the Investigator. Leukopenia due to a clinical or pathological process, unless leukopenia is directly attributable to a transient process (e.g., acute viral infection) and resolution has been documented at time of enrollment. Any screening or baseline laboratory test which in the opinion of the Investigator, is considered clinically significant. Any cytotoxic therapy in the previous 5 years. Diabetes mellitus (type I or II), with the exception of previous gestational diabetes. Any chronic or active neurologic disorder, such as migraines (including silent), seizures or epilepsy (excluding a single febrile seizure as a child as judged by the Investigator). Have a known history of Guillain Barré Syndrome (GBS). Have an active malignancy or history of metastatic or hematologic malignancy. Suspected or known alcohol and/or illicit drug abuse within the past 5 years per the judgment of the Investigator. Moderate or severe illness and/or fever >100.4 °F within 1 week prior to vaccination (subjects can be rescheduled). Administration of immunoglobulin G (IgGs) and/or any blood products within the 120 days preceding study entry or planned administration during the study period. History of blood donation within 60 days of enrollment or plans to donate within the study period. Administration of chronic (defined as more than 14 days) immunosuppressants or other immune modifying drugs within 6 months of study entry: for corticosteroids, this includes chronic oral >14 days or intraarticular steroids in the past 6 months (intranasal and topical are allowed). Major surgery or hospitalization planned during the period of study participation. Research staff or the immediate family of research staff directly involved with the clinical study. Any other significant finding that in the opinion of the Investigator that would increase the risk of the individual having an adverse outcome from participating in this study.
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Murray A Kimmel, DO
Organizational Affiliation
Optimal Research, LLC
Official's Role
Principal Investigator
Facility Information:
Facility Name
Optimal Research
City
Melbourne
State/Province
Florida
ZIP/Postal Code
32934
Country
United States

12. IPD Sharing Statement

Plan to Share IPD
No
Citations:
PubMed Identifier
31952923
Citation
Clarke DK, Xu R, Matassov D, Latham TE, Ota-Setlik A, Gerardi CS, Luckay A, Witko SE, Hermida L, Higgins T, Tremblay M, Sciotto-Brown S, Chen T, Egan MA, Rusnak JM, Ward LA, Eldridge JH. Safety and immunogenicity of a highly attenuated rVSVN4CT1-EBOVGP1 Ebola virus vaccine: a randomised, double-blind, placebo-controlled, phase 1 clinical trial. Lancet Infect Dis. 2020 Apr;20(4):455-466. doi: 10.1016/S1473-3099(19)30614-0. Epub 2020 Jan 14.
Results Reference
derived

Learn more about this trial

Trial to Evaluate Safety and Immunogenicity of an Ebola Zaire Vaccine in Healthy Adults

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