Microbiome Shift in Peri-mucositis by Anti-inflammatory Drugs
Primary Purpose
Peri-implant Mucositis
Status
Unknown status
Phase
Early Phase 1
Locations
Israel
Study Type
Interventional
Intervention
Flurbiprofen
Placebo Oral Tablet
Sponsored by
About this trial
This is an interventional diagnostic trial for Peri-implant Mucositis focused on measuring Peri-implant Mucositis, NSAID, Microbiome, 16s Ribosomal Pyro-sequencing
Eligibility Criteria
Inclusion Criteria:
- clinical diagnosis of Peri-implant Mucositis
Exclusion Criteria:
- hypersensitivity to or intolerance of Flurbiprofen or NSAIDs
- peptic ulcer
- pregnancy
- smoking
- diabetes mellitus
- having taken any antibiotic drug within the previous 6 weeks
Sites / Locations
- Hadassah Medical Organization
Arms of the Study
Arm 1
Arm 2
Arm Type
Active Comparator
Placebo Comparator
Arm Label
Flurbiprofen
Placebo
Arm Description
Flurbiprofen 100 mg bid.
Placebo taken bid
Outcomes
Primary Outcome Measures
Change in the Microbiome
The microbiome profile of the Flurbiprofen group before treatment (base line) and after treatment (day 14) and two weeks post-treatment (day 28) will be compared, as well as the microbiome of the placebo group. data will be pooled and analysed as absolute number of any microbe within the tested biofilms. Measures of change will be set as 14d vs. baseline and 28 day vs. baseline.
Secondary Outcome Measures
change in periodontal probing depth
change in probing sulcular depth around implants
change in bleeding on probing
change in bleeding on probing in the peri-implant sulcus
change in Suppuration
change in presence or absence in the peri-implant sulcus
Full Information
1. Study Identification
Unique Protocol Identification Number
NCT03090009
Brief Title
Microbiome Shift in Peri-mucositis by Anti-inflammatory Drugs
Official Title
The Use of Non-Steroidal Anti-Inflammatory Drugs as a Tool of Positive Change in Peri-Implants Mucositis Microbiome
Study Type
Interventional
2. Study Status
Record Verification Date
March 2017
Overall Recruitment Status
Unknown status
Study Start Date
April 2017 (Anticipated)
Primary Completion Date
April 2019 (Anticipated)
Study Completion Date
April 2020 (Anticipated)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Sponsor-Investigator
Name of the Sponsor
Polak David
4. Oversight
Studies a U.S. FDA-regulated Drug Product
Yes
Studies a U.S. FDA-regulated Device Product
No
Product Manufactured in and Exported from the U.S.
No
Data Monitoring Committee
No
5. Study Description
Brief Summary
Dental implants are often used to replace missing teeth. In fact, in the US over 700,000 implants are places every year and over 2 million implants are places world wide. Peri-implant mucositis in an inflammatory condition affecting dental implants and is recognized as a risk factor for peri-implantitis (a condition affecting the bone around implants and eventually leading to implant loss). The prevalence of Peri-implant Mucositis has been reported in the literature to range from 50-90% whereas the prevalence of Peri-implantitis has been reported as high as 20%. it is commonly believed that a dysbiotic microbiome is the primary cause for these conditions.
The inflammatory burden around diseased implants creates a high-protein environment which is necessary for the survival of pathogenic bacteria. It is logical, therefore, that reducing inflammation by Non Steroidal Anti-Inflammatory Drugs (NSAIDs) may create a shift in the dysbiotic microbiome to a symbiotic microbiome. The aim of the current study is to test the effects of oral NSAIDs on the peri-implant microbiome.
Detailed Description
Inflammation around dental implants has been defined as Peri-implant mucositis and Peri-implantitis . Peri-implant mucositis is a localized inflammation limited to the mucosal tissue surrounding the implant whereas peri-implantitis has advanced to the bone surrounding the implant as well. The prevalence of Peri-implant Mucositis has been reported in the literature to range from 50-90% whereas the prevalence of Peri-implantitis has been reported as high as 20%. Due to the fact that there are many similarities in the clinical manifestations of infection in the gingiva and peri-implant mucosal tissue, studies attempted to compare the microbial characteristics around teeth to that found around implants. Emerging data suggests that the microbiome around infected teeth has some similarities and dissimilarities to peri-implant infection. Such differences may stem from different inflammatory characteristics as well as the presence of a non-vital foreign material (the dental implant) in the area. Some of the known pathogenic strains identified in the peri-implant sulcus are anaerobic a-saccharolytic bacteria, which feed off the adjacent tissue inflammatory exudate. Logically, attenuation of the inflammation would deprive the nearby bacteria of nourishment needed to sustain their growth.
The use of non-steroidal anti-inflammatory drugs (NSAID) to control chronic inflammatory disease has been used extensively in medicine. Its action of blocking cyclooxygenase inflammatory pathway and in-effect blocking the production of Prostaglandin-E2 (PGE2) has led to its use with general pain control as well as its long term use in the treatment of tissue inflammation and pain caused by rheumatoid arthritis or osteoarthritis, tendinitis and bursitis.
Offenbacher et al. as early as in 1981 discovered that patients with periodontitis had significantly higher levels of PGE2 in the sulcular fluid between gingiva and the tooth (GCF) . In a follow up study over a period of 18-36 months, Offenbacher observed that patients who had exhibited periodontal attachment loss had significantly higher levels of PGE2. This led Williams et al. to study the effects of Flurbiprofen, a phenylalkanoic derivative family of of non-steroidal anti-inflammatory drugs, on naturally occurring periodontal disease in an animal model over a period of 12 months. The study demonstrated decreases in alveolar bone loss in flurprofen treated animals at 3, 6 , 9 and 12 months. William et al then followed with a clinical trial where 44 patients who self administered peroral flurbiprofen twice daily for a period of 24 months. The study reported severely depressed bone loss rates over the period of the trial and as early as 6 months after administration of flurbiprofen that continued over the length of the trial. Other studied the drugs effect on other more aggressive forms of the disease with similar results . These results have been duplicated around implants as well. Jeffcoat et al demonstrated that oral intake flurbiprofen helped reduce bone loss around implants in the first year of service. Reddy similarly demonstrated with use of digital subtraction radiography, flurbiprofen taken orally was able to help increase bone density and apposition around implants after just 4 months.
The positive effect of systemic antibiotics and as well topical anti microbials on the pathogenic bacteria in the gingiva sulcus and peri-implant crevice has been well documented and is indisputable. The removal of these bacteria has helped promote tissue health. Similar results have been documented around implants as well. However more recently the reverse approach to disease control has gained popularity. Instead of trying just to reduce the bacterial challenge through antimicrobial/antibiotic therapy, modulating the host response to decrease the inflammatory destruction of the tissue, could be a more efficient and effective way of controlling the disease In decreasing the inflammatory environment, the pathogenic bacteria feeding off the inflammatory exudate would be greatly decreased, effecting a major change in the microbiome. To date nobody has characterized this change.
The purpose of the study is to test the effects of oral NSAIDS on the peri-implant microbiome. Also, clinical parameters will be tested for correlation with the microbial findings.
Methods:
The study design is random controlled double blind clinical trial. Patients visiting the graduate periodontal clinic at the Hadassah Medical Center will be examined for eligibility for the study. Implants with peri-implant mucositis (clinical signs of inflammation without radiographically bone loss) will be included. Patients (n=50) will undergo clinical examination (which will include probing depths, presence or absence of suppuration and bleeding on probing) and sampling of the subgingival bacterial deposits using a perio-strip. Patients will be randomly assigned to the experimental flurbiprofen group (100 mg bid) or placebo group (n=25 for each group). Drug consumption will be set for the duration of 2 weeks. At the end of drug intake period (day 14) and two weeks afterwards (day 28) all subjects will be re-examined, which will include a new clinical exam and sampling of the subgingival bacterial deposits. The microbiological samples will be analyzed using 16s Ribosomal Pyro-sequencing. The microbiome profile of the Flurbiprofen group before and after treatment and two weeks post-treatment will be compared. as well as the microbiome from the placebo group compared with the Flurbiprofen group. Statistical differences will be evaluated using ANOVA with Bonferroni correction. Correlation between the clinical parameters and microbiological parameters will be tested using Pearson correlation.
Significance:
The study asks the question whether the use of a well-known NSAID that had proven beneficial effects in maintaining the mucosal tissue integrity around implants and help control and prevent peri-implant inflammation. Studies have shown that implants placed 5 years previously will most likely have some form of peri-implant mucositis . Just as gingivitis can lead to periodontal disease, Peri-implant mucositis can lead to peri-implantitis a more destructive inflammatory disease that puts implant survival in jeopardy. Therefore, a drug that can limit the amount of destructive bacteria around the implant can be a very effective non-invasive method of treatment. Additionally with the use of a more comprehensive taxonomical identification of the peri-implant micro-genome, there will be a better understanding of the bacterial role in the destructive process associated with peri-implant inflammation
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Peri-implant Mucositis
Keywords
Peri-implant Mucositis, NSAID, Microbiome, 16s Ribosomal Pyro-sequencing
7. Study Design
Primary Purpose
Diagnostic
Study Phase
Early Phase 1
Interventional Study Model
Parallel Assignment
Model Description
The study design is random controlled double blind clinical trial
Masking
ParticipantInvestigator
Masking Description
Both patient and investigator will blinded to the identity of the medication given.
Allocation
Randomized
Enrollment
100 (Anticipated)
8. Arms, Groups, and Interventions
Arm Title
Flurbiprofen
Arm Type
Active Comparator
Arm Description
Flurbiprofen 100 mg bid.
Arm Title
Placebo
Arm Type
Placebo Comparator
Arm Description
Placebo taken bid
Intervention Type
Drug
Intervention Name(s)
Flurbiprofen
Other Intervention Name(s)
Ansaid, Strepfen, Ocufen
Intervention Description
100 mg twice a day for 14 days
Intervention Type
Drug
Intervention Name(s)
Placebo Oral Tablet
Other Intervention Name(s)
Placebo
Intervention Description
placebo group
Primary Outcome Measure Information:
Title
Change in the Microbiome
Description
The microbiome profile of the Flurbiprofen group before treatment (base line) and after treatment (day 14) and two weeks post-treatment (day 28) will be compared, as well as the microbiome of the placebo group. data will be pooled and analysed as absolute number of any microbe within the tested biofilms. Measures of change will be set as 14d vs. baseline and 28 day vs. baseline.
Time Frame
28 days
Secondary Outcome Measure Information:
Title
change in periodontal probing depth
Description
change in probing sulcular depth around implants
Time Frame
28 days
Title
change in bleeding on probing
Description
change in bleeding on probing in the peri-implant sulcus
Time Frame
28 days
Title
change in Suppuration
Description
change in presence or absence in the peri-implant sulcus
Time Frame
28 days
10. Eligibility
Sex
All
Minimum Age & Unit of Time
18 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria:
clinical diagnosis of Peri-implant Mucositis
Exclusion Criteria:
hypersensitivity to or intolerance of Flurbiprofen or NSAIDs
peptic ulcer
pregnancy
smoking
diabetes mellitus
having taken any antibiotic drug within the previous 6 weeks
Central Contact Person:
First Name & Middle Initial & Last Name or Official Title & Degree
David Polak, DMD PhD
Phone
972-2-6777826
Email
polak@mail.huji.ac.il
First Name & Middle Initial & Last Name or Official Title & Degree
Aron J Saffer, DDS MS
Phone
972-544481897
Email
dr-sapir@jerusalemperio.com
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
David Polak, DMD PhD
Organizational Affiliation
Hadassah Medical Organization
Official's Role
Principal Investigator
Facility Information:
Facility Name
Hadassah Medical Organization
City
Jerusalem
Country
Israel
12. IPD Sharing Statement
Plan to Share IPD
No
Citations:
PubMed Identifier
11276870
Citation
Paquette DW, Williams RC. Modulation of host inflammatory mediators as a treatment strategy for periodontal diseases. Periodontol 2000. 2000 Oct;24:239-52. doi: 10.1034/j.1600-0757.2000.2240112.x.
Results Reference
background
PubMed Identifier
7646652
Citation
Jeffcoat MK, Reddy MS, Wang IC, Meuninghoff LA, Farmer JB, Koth DL. The effect of systemic flurbiprofen on bone supporting dental implants. J Am Dent Assoc. 1995 Mar;126(3):305-11; quiz 346-7. doi: 10.14219/jada.archive.1995.0173.
Results Reference
background
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Microbiome Shift in Peri-mucositis by Anti-inflammatory Drugs
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