Identification of TRP Channels as New Potential Therapeutic Targets in Primary and Secondary Raynaud's Phenomenon. (TRP)
Raynaud's Phenomenon Isolated Primary, Raynaud's Phenomenon (Secondary), Healthy
About this trial
This is an interventional basic science trial for Raynaud's Phenomenon Isolated Primary focused on measuring Transient Receptor Potential Channels, Raynaud's Phenomenon Isolated Primary, Raynaud's Phenomenon (Secondary)
Eligibility Criteria
Inclusion Criteria:
- Healthy volunteers :dated and signed written consent
- Primary Raynaud's patients :
- dated and signed written consent
- subject presenting a primary Raynaud's phenomenon non associated to a connectivite. RP crisis are defined by a succession of caracteristic symptoms : a syncopal phase with distal whitening of the fingers, usually followed by a cyanotic phase, and then a hyperhemic phase with the outbreak of painful redness.The syncopal phase is always present, while the two other phases can be less pronounced, or even absent.
- Sclerodermy secondary Raynaud's patients :
- dated and signed written consent
- subject with Raynaud's phenomenon secondary to systemic sclerosis, according to the 2013 EULAR/ACR criteria.
Exclusion Criteria:
- Minor person or major person protected by the law
- Exclusion period for any clinical trial including a medication or focusing on a clinical device linked to the pathology
- Social secutrity scheme non-affiliated person or person who cannot pretend to such a scheme
- Pregnant woman, parturient or breast-feeding mother
- Person displaying a digital ulcer or a history of digital ulcer
- Severe concurrent disease found at oral questioning : evolutive cancer, hepatic insufficiency, history of myocardic infarction in the past five years, angina pectoris
- Hemodynamic instability
- Any tobacco consumption
- Any contraindication to the medication used in this trial :
- Anesderm ® (lidocain/procain) : Known hypersensitivity, to local amide linking anesthetic or to any other compound of the cream, congenital methemoglobin porphyria
- Lidocain Aguettant 5 mg/ml without preservative, injectable solution : Known hypersensitivity to lidocain chlorhydrate, to local amide linking anesthetic or to one of its excipients, patients affected with reccurrent porphyrias, administration using intravenous way for children under 5 years
- Xylocain 10 mg/ml epinephrine 0.005 mg/ml, injectable solution : Known hypersensitivity to lidocain chlorhydrate, to local amide linking anesthetic or to one of its excipients, patients affected with reccurrent porphyrias, administration using intravenous way for children under 5 years, linked to the epinephrined compound : intra vascular way, angina pectoris, ventricular rythm troubles, severe arterial hypertension, obstructive cardiomyopathy, hyper thyroidy, anesthesia with local infiltration on the extermities (fingers, penis).
- Kalinox ® (MEOPA) : Need for ventilation in pure oxygen, intracranial hypertension, alteration of consciousness, cranial trauma, pneumothorax, emphysema bubble, submarine diving accident, intestinal gas distension, gas bubble from an eye surgery under 3 months, known and non substituted B12 vitamine or folic acid deficiency, neurological disorder of recent onset and non explained.
- Person deprived from his/her freedom on a judicial or administrative decision, person under a legal protection measure.
Sites / Locations
- CHU of Grenoble Alpes
Arms of the Study
Arm 1
Arm 2
Arm 3
Experimental
Experimental
Experimental
Healthy
Primary Raynaud's phenomenon
Secondary Raynaud's phenomenon
Two sequences of ten healthy volunteers each will be performed : During the first sequence, a biopsy of the gluteal area and another of the surrounding nail area of a finger will be sampled.Then, the cutaneous blood flux of the hand will be recorded at rest and during cooling and warming periods. During the second sequence, two other biopsies of the surrounding nail area of two other fingers will be sampled.
Two sequences of ten primary Raynaud's syndrome each will be performed : During the first sequence, a biopsy of the gluteal area and another of the surrounding nail area of a finger will be sampled.Then, the cutaneous blood flux of the hand will be recorded at rest and during cooling and warming periods. During the second sequence, two other biopsies of the surrounding nail area of two other fingers will be sampled.
Two sequences of ten patients each will be performed : During the first sequence, a biopsy of the gluteal area and another of the surrounding nail area of a finger will be sampled.Then, the cutaneous blood flux of the hand will be recorded at rest and during cooling and warming periods. During the second sequence, two other biopsies of the surrounding nail area of two other fingers will be sampled.