Casein Phosphopeptide--amorphous Calcium Phosphate on Human Enamel Subjected to in Vivo Acid Attacks
Primary Purpose
Dental Erosion
Status
Completed
Phase
Phase 4
Locations
Study Type
Interventional
Intervention
CPP-ACP
Placebos
Sponsored by
About this trial
This is an interventional prevention trial for Dental Erosion
Eligibility Criteria
Inclusion Criteria:
- 18 to 60 years of age and in good general health
- Dental erosion and wear degree is 0-1
Exclusion Criteria:
- Systemic diseases or oral mucosal disorders
- Current orthodontic treatment
- Pregnancy
- Known allergies to the experimental drink
- Had been given drugs which lead to dry oral more regularly within half a year
Sites / Locations
Arms of the Study
Arm 1
Arm 2
Arm Type
Experimental
Placebo Comparator
Arm Label
CPP-ACP
Deionized water
Arm Description
Tooth mousse (GC, Japan) application on the specimen surface for 3 min.
Deionized water application on the specimen surface for 3 min
Outcomes
Primary Outcome Measures
Surface microhardness
a Vickers microhardness tester with microscopic lens was used, with a 100 g load and 30-s dwell time. Three microhardness measurements were obtained on the top surface of each specimen.
Surface microhardness
a Vickers microhardness tester with microscopic lens was used, with a 100 g load and 30-s dwell time. Three microhardness measurements were obtained on the top surface of each specimen.
Secondary Outcome Measures
Full Information
NCT ID
NCT03426150
First Posted
January 24, 2018
Last Updated
February 6, 2018
Sponsor
Fujian Medical University
1. Study Identification
Unique Protocol Identification Number
NCT03426150
Brief Title
Casein Phosphopeptide--amorphous Calcium Phosphate on Human Enamel Subjected to in Vivo Acid Attacks
Official Title
In Situ Effect of Casein Phosphopeptide--amorphous Calcium Phosphate on the Surface Microhardness of Human Enamel Subjected to in Vivo Acid Attacks
Study Type
Interventional
2. Study Status
Record Verification Date
February 2018
Overall Recruitment Status
Completed
Study Start Date
December 25, 2015 (Actual)
Primary Completion Date
October 25, 2017 (Actual)
Study Completion Date
February 5, 2018 (Actual)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
Fujian Medical University
4. Oversight
Studies a U.S. FDA-regulated Drug Product
No
Studies a U.S. FDA-regulated Device Product
No
Product Manufactured in and Exported from the U.S.
No
Data Monitoring Committee
Yes
5. Study Description
Brief Summary
40 specimens were prepared from fresh-extracted human molars and further embedded using the acrylic resin. Specimens were randomly divided into 2 groups according to the treatment before in vivo erosion (n=20): CPP-ACP for 3 min (CPP-ACP group) and deionized water for 3 min (control group). 10 healthy volunteers were recruited and customized maxillary appliance containing 4 specimens (2 from CPP-ACP group and 2 from control group) was fabricated for each volunteer. Participants were instructed to drink 150 ml cola in 5 min using the gargling method after placing appliances intraorally for 2 h. The in vivo attacks were performed 4 X 5 min with 1-h interval daily over 7 d. The surface microhardness and surface microstructure of the specimens were evaluated at the baseline and after the treatment. The data were statistically analyzed using three-way ANOVA and post hoc tests. Data were considered statistically significant at a level of P <0.05.
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Dental Erosion
7. Study Design
Primary Purpose
Prevention
Study Phase
Phase 4
Interventional Study Model
Crossover Assignment
Masking
Participant
Allocation
Randomized
Enrollment
12 (Actual)
8. Arms, Groups, and Interventions
Arm Title
CPP-ACP
Arm Type
Experimental
Arm Description
Tooth mousse (GC, Japan) application on the specimen surface for 3 min.
Arm Title
Deionized water
Arm Type
Placebo Comparator
Arm Description
Deionized water application on the specimen surface for 3 min
Intervention Type
Drug
Intervention Name(s)
CPP-ACP
Intervention Description
The surfaces of specimens were pretreated with CPP-ACP for 3 min
Intervention Type
Drug
Intervention Name(s)
Placebos
Intervention Description
The surfaces of specimens were pretreated with deionized water for 3 min
Primary Outcome Measure Information:
Title
Surface microhardness
Description
a Vickers microhardness tester with microscopic lens was used, with a 100 g load and 30-s dwell time. Three microhardness measurements were obtained on the top surface of each specimen.
Time Frame
baseline
Title
Surface microhardness
Description
a Vickers microhardness tester with microscopic lens was used, with a 100 g load and 30-s dwell time. Three microhardness measurements were obtained on the top surface of each specimen.
Time Frame
7 days
10. Eligibility
Sex
All
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
60 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria:
18 to 60 years of age and in good general health
Dental erosion and wear degree is 0-1
Exclusion Criteria:
Systemic diseases or oral mucosal disorders
Current orthodontic treatment
Pregnancy
Known allergies to the experimental drink
Had been given drugs which lead to dry oral more regularly within half a year
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Hao Yu, DDS, PhD
Organizational Affiliation
School and Hospital of Stomatology, Fujian Medical University
Official's Role
Principal Investigator
12. IPD Sharing Statement
Plan to Share IPD
Undecided
Citations:
PubMed Identifier
29857075
Citation
Yu H, Jiang NW, Ye XY, Zheng HY, Attin T, Cheng H. In situ effect of Tooth Mousse containing CPP-ACP on human enamel subjected to in vivo acid attacks. J Dent. 2018 Sep;76:40-45. doi: 10.1016/j.jdent.2018.05.021. Epub 2018 May 30.
Results Reference
derived
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Casein Phosphopeptide--amorphous Calcium Phosphate on Human Enamel Subjected to in Vivo Acid Attacks
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