Quantitative Analysis of Global Proteome in Bone Samples From Patients With Osteoporotic and Nonosteoporotic Fracture

Quantitative Analysis of Global Proteome in Bone Samples From Patients With Osteoporotic and Nonosteoporotic Fracture

RATIONALE: Collecting and storing bone samples from patients with osteoporotic and nonosteoporotic fracture to quantitatively analyze the differentially expressed proteins to further explain the relationship between bone iron content and bone mineral density (BMD). PURPOSE: This research study is looking at changes of iron content and differentially expressed proteins in bone samples from patients with osteoporotic and nonosteoporotic. fracture.

OBJECTIVES: Establish the iron content in bone specimen in patients with hip fracture. Explore the correlation of BMD and differentially expressed proteins. OUTLINE: Collect human bone specimens from patients with osteoporotic and nonosteoporotic Fracture. Provide a repository for storage of tissue and make these specimens available for approved projects by laboratory-based investigators. Collect clinical data on these patients including bone mineral density. To examine the bone iron content using ICP/MS. Investigate the differentially expressed proteins through quantitative analysing the global proteome of osteoporotic and nonosteoporotic fracture bone specimens.

patients with osteoporotic fractures, including femoral neck fracture and pertrochanteric fracture. Total hip arthroplasty or proximal femoral nails was needed.

patients with nonosteoporotic fractures, including femoral neck fracture and pertrochanteric fracture. Total hip arthroplasty or proximal femoral nails was needed.

Bone samples were preprocess and decomposed by microwave.Icp-ms is used for quantitative detection of samples.

Bone mineral density was measured by double energy X-ray absorptiometer (DXA) in a week before surgery.

Find the differentially expressed proteins in nonosteoporotic or osteoporotic bone sample through using an integrated approach involving TMT labeling and LC-MS/MS to quantify the dynamic changes of the whole proteome of Human Species.

Inclusion Criteria: Ages Eligible for Study: 55-90 Years Genders Eligible for Study: Woman Accepts Healthy Volunteers: NO Study Population: Female patients with hip fracture. Exclusion Criteria: Malignancy or benign ovarian cysts Known chronic or systemic diseases Hormone therapy in the previous 3 months Bone metabolism and drug therapy

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