Identification by Cytometry by Mass of Predictive Immunological Profiles of Answer to Treatmentby Biotherapics for Patients With Crohn's Disease (PICTURE)

Identification by Cytometry by Mass of Predictive Immunological Profiles of Answer to Treatmentby Biotherapics for Patients With Crohn's Disease

Identification by Cytometry by Mass of Predictive Immunological Profiles of Answer to Treatmentby Biotherapics for Patients With Crohn's Disease

Crohn disease is an inflammatory chronic disease of the bowel the complex physiopathology of which brings in immunological, genetic and environmental factors. At present, the appeal to biotherapics anti-TNFa (infliximab and adalimumab) or anti-IL-12/23 (ustekinumab) in MC represents a major therapeutic progress at the origin of a significant improvement of the symptoms, the healing of the intestinal hurts and the quality of life. Considering the new immunological targets of these biotherapics, the investigators put the hypothesis that an immunological profile (impulsive person and\or tissular) specific of the patients with one MC is associated with the answer to biotherapics. So, before beginning the treatment the patients expressing strongly the pro-inflammatory cytokine TNFa, would more may be answering machines in anti-TNFa and those with whom the immunological profile is very marked towards the way Th1/Th17 would more may answer favorably the ustekinumab. The identification of immunological profiles capable of predicting before treatment the answer under biotherapics could establish in MC but also in other inflammatory diseases a major step forward to guide the coverage.

Identify by cytometry of mass a predictive, blood and/or mucous immunological profile, of the answer at week 14 to a biotherapics by anti-TNFa or anti IL-12 / 23 at suffering patients of Crohn disease in relapse

Measurements of immunological markers by mass cytometry will be performed in the blood at baseline and at week 14 and in the gastrointestinal mucosa (endoscopic biopsies) only at baseline.

Assays for inflammatory blood (CRP) and fecal markers (fecal calprotectin) will be performed to predict the response to anti-TNFα or ustekinumab (anti IL-12/23) at inclusion and at week 14, with conventional methods used routinely.

Inclusion Criteria: Patient of more than 18 years old Patient presenting a MC before diagnosed according to the usual criteria Patient in relapse (score CDAI > 150) for at least 1 month requiring Patient a treatment by anti-TNF or ustekinumab according to the indications of the AMM Patient compatible with the realization of endoscopic digestive biopsies Patient having signed a consent of participation Exclusion Criteria: -· patient with Crohn disease with a CDAI score < 150 · contraindication to anti-TNF and\or ustekinumab · contraindication to the realization of an iléocoloscopie ·- Patient taking anti-inflammatory drugs or antibiotics · Patient presenting complications of the type intestinal sub-occlusion, fistulas or abdominal abscesses · Patient having a exclusive perianal disease or having pregnant Women · Patients having been the object of a vast intestinal resection · Patient with an ileostomy or a colostomy · No consent of the patient · patient under legal protection · Subject participating in another research which need a period of exclusion