Transcriptome Assessment After Cardiac Arrest (OMECARD)

" Etude du Transcriptome Complet Lors du Syndrome Post-arrêt Cardiaque " Transcriptome Assessment After Cardiac Arrest

National Research Agency, France, University of Paris 5 - Rene Descartes, Institut National de la Santé Et de la Recherche Médicale, France, Université Paris Est Créteil, Ecole Nationale Vétérinaire d'Alfort

The purpose of this study is to determine whether the blood transcriptome of patients resuscitated after out-of-hospital could be an early predictor of the neurological outcome.

Assessing the prognosis of the patients resuscitated after out-of-hospital cardiac arrest (OHCA) is still highly challenging. Beyond usual neurological markers, experimental and clinical studies have also shown that blood levels of several cytokines or miRNA could be significantly different after cardiac arrest in subjects prematurely died as compared to survivors with a good neurological recovery. Here the investigators propose to investigate a screening approach assessing simultaneously multiple targets of the post-cardiac arrest syndrome through analysis of the whole transcriptome of patients after OHCA. For this purpose, the investigators will collect blood samples from 60 patients after admission at hospital and then 1 and 3 days later. The Pittsburgh Cerebral Performance Category of each patient will be evaluated for each patient at day 60 after cardiac arrest. At the end of the collection period, the blood samples will be prepared for whole genome analysis and RNA sequencing. The primary analysis of the investigators will be the comparison of the transcriptome at day 0 after cardiac arrest in patients reaching Cerebral Performance Category (CPC) 1 at day 60 as compared to all other patients.

Transcriptome, messenger ribonucleic acid (mRNA), sequencing, biomarker, cardiac arrest, prognostication, successful resuscitation

Blood samples will be collected for each patient early after admission at hospital and blood whole transcriptome will be assessed by RNA sequencing

Blood samples will be collected for each patient at day 1 and blood whole transcriptome will be assessed by RNA sequencing

Blood samples will be collected for each patient at day 3 and blood whole transcriptome will be assessed by RNA sequencing

Inclusion Criteria: Age between 18 and 80 years Delay between patients collapse and resumption of spontaneous circulation (ROSC) < 60 minutes Comatose patients ROSC with Glasgow score < 7 Patient already registered in the social security system Consent of a family member, if able to understand in the emergency context, or attestation of emergency situation with a follow-up information notice Exclusion Criteria: Cardiac arrest supposed to be provoked by trauma or sepsis