Effects of Ephedrine, Phenylephrine, Norepinephrine and Vasopressin on Contractility of Human Myometrium and Umbilical Vessels: An In-vitro Study
Hypotension
About this trial
This is an interventional treatment trial for Hypotension focused on measuring vasopressor, ephedrine, phenylephrine, norepinephrine, umbilical arteries, uterine contraction, fetal acidosis, vasopressin
Eligibility Criteria
Inclusion Criteria:
- Patients who give written consent to participate in this study
- Patients with gestational age 37-41 weeks
- Patients of 19-40 years
- Non-laboring patients, not exposed to exogenous oxytocin
- Patients requiring elective primary or first repeat caesarean delivery
- Patients undergoing caesarean delivery under spinal anesthesia
Exclusion Criteria:
- Patients who refuse to give written informed consent
- Patients who require general anesthesia
- Patients in labor and those receiving oxytocin for induction of labor
- Emergency caesarean delivery in labor
- Patients who have had previous uterine surgery or >1 previous caesarean delivery
- Patients with any condition predisposing to uterine atony
- Patients on medications that could affect myometrial contractility, such as insulin, nifedipine, labetolol or magnesium sulfate.
Sites / Locations
- Mount Sinai HospitalRecruiting
Arms of the Study
Arm 1
Arm 2
Arm 3
Arm 4
Arm 5
Arm 6
Arm 7
Arm 8
Arm 9
Arm 10
Arm 11
Arm 12
Experimental
Experimental
Experimental
Experimental
Experimental
Experimental
Experimental
Experimental
Experimental
Experimental
Experimental
Experimental
Myometrium + Ephedrine
Myometrium + Phenylephrine
Myometrium + Norepinephrine
Myometrium + Vasopressin
Umbilical artery + Ephedrine
Umbilical artery + Phenylephrine
Umbilical artery + Norepinephrine
Umbilical artery + Vasopressin
Umbilical vein + Ephedrine
Umbilical vein + Phenylephrine
Umbilical vein + Norepinephrine
Umbilical vein + Vasopressin
The myometrial samples are bathed in physiological salt solution (PSS) with increasing concentrations of ephedrine
The myometrial samples are bathed in physiological salt solution (PSS) with increasing concentrations of phenylephrine
The myometrial samples are bathed in physiological salt solution (PSS) with increasing concentrations of norepinephrine
The myometrial samples are bathed in physiological salt solution (PSS) with increasing concentrations of vasopressin
The umbilical artery samples are bathed in physiological salt solution (PSS) with increasing concentrations of ephedrine
The umbilical artery samples are bathed in physiological salt solution (PSS) with increasing concentrations of phenylephrine
The umbilical artery samples are bathed in physiological salt solution (PSS) with increasing concentrations of norepinephrine
The umbilical artery samples are bathed in physiological salt solution (PSS) with increasing concentrations of vasopressin
The umbilical vein samples are bathed in physiological salt solution (PSS) with increasing concentrations of ephedrine
The umbilical vein samples are bathed in physiological salt solution (PSS) with increasing concentrations of phenylephrine
The umbilical vein samples are bathed in physiological salt solution (PSS) with increasing concentrations of norepinephrine
The umbilical vein samples are bathed in physiological salt solution (PSS) with increasing concentrations of vasopressin