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Allogeneic CD30 Chimeric Antigen Receptor Epstein-Barr Virus-Specific T Lymphocytes in Relapsed or Refractory CD30-Positive Lymphomas

Primary Purpose

Extranodal Natural Killer/T-Cell Lymphoma, Nasal Type, Classical Hodgkin Lymphoma

Status
Withdrawn
Phase
Phase 1
Locations
United States
Study Type
Interventional
Intervention
CD30.CAR-EBVST cells
Sponsored by
Baylor College of Medicine
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional treatment trial for Extranodal Natural Killer/T-Cell Lymphoma, Nasal Type focused on measuring CD30-Positive Lymphoma, Hodgkin lymphoma, Non-Hodgkin lymphoma, CD30 CAR

Eligibility Criteria

12 Years - 75 Years (Child, Adult, Older Adult)All SexesDoes not accept healthy volunteers

Inclusion Criteria:

  1. Diagnosis and clinical course falling into one of the following categories:

    1. Hodgkin lymphoma
    2. Aggressive non-Hodgkin lymphoma
    3. ALK-negative anaplastic T cell lymphoma or other peripheral T-cell lymphoma
    4. ALK-positive anaplastic T cell lymphoma
  2. CD30-positive tumor as assayed in a CLIA certified Pathology Laboratory.
  3. Age 12 to 75.
  4. Bilirubin 2 times (or 3 times if the patient has Gilbert syndrome) or less than the upper limit of normal.
  5. AST 3 times or less than the upper limit of normal.
  6. Estimated GFR > 70 mL/min.
  7. Pulse oximetry of > 90% on room air
  8. EKG shows no significant arrhythmias
  9. Karnofsky or Lansky score of > 60%.
  10. Available allogeneic T cells with ≥15% expression of CD30CAR determined by flow-cytometry.
  11. Recovered from all acute non-hematologic toxic effects of all prior chemotherapy.
  12. Sexually active patients must be willing to utilize one of the more effective birth control methods during the study and for 6 months after the study is concluded. The male partner should use a condom.
  13. Informed consent explained to, understood by and signed by patient or guardian. Patient or guardian given a copy of the informed consent form.

Exclusion Criteria:

  1. Currently receiving any investigational agents or received any tumor vaccines within the previous six weeks.
  2. Received CD30 antibody-based therapy within the previous 4 weeks.
  3. History of hypersensitivity reactions to murine protein-containing products.
  4. Pregnant or lactating.
  5. Tumor in a location where enlargement could cause airway obstruction.
  6. Current use of systemic corticosteroids at a dose equivalent to higher than 10 mg/day of prednisone.
  7. Active significant, uncontrolled bacterial, viral or fungal infection.
  8. Symptomatic cardiac disease (NYHA Class III or IV disease).

Sites / Locations

  • Houston Methodist Hospital
  • Texas Children's Hospital

Arms of the Study

Arm 1

Arm Type

Experimental

Arm Label

Treatment Phase

Arm Description

Three dose levels will be evaluated based on safety data from our current study of CD30 CAR T cells. Cohorts of three to six patients will be enrolled at each dose level The dose is based on the number of CD.30 CAR-EBVT-expressing cells administered. The total number of dose levels evaluated will depend upon toxicities experienced. Dose level cohorts will be numbered sequentially. Dose Level 1: 1 × 10^8 CD30.CAR-EBVST cells Dose Level 2: 4 × 10^8 CD30.CAR-EBVST cells Dose Level 3: 1 × 10^9 CD30.CAR-EBVST cells

Outcomes

Primary Outcome Measures

Dose limiting toxicity rate (DLT) by CTCAE 5.0
Any Grade 5 event, / Non-hematologic dose-limiting toxicity is any Grade 3 or Grade 4 non-hematologic toxicity that fails to return to Grade 2 within 72 hours, / Grade 2-4 allergic reaction to T-Cells, / Grade 3-4 GVHD, / Grade 3-4 CRS. Toxicity will be evaluated according to the CTCAE Version 5.0. GVHD will be graded by the method of Przepiorka et al.

Secondary Outcome Measures

Rate of Anti-Tumor effect Objective Response (OR)
Objective response rate is defined as complete response and partial response
Duration of Response
Response duration will be measured from the time of initial response until documented tumor progression.
Stable disease (SD) rate
SD will be defined as the proportion of patients that have stable disease
Duration of SD
Stable disease is measured from the start of the treatment until the criteria for progression are met.
Progression free survival (PFS)
PFS is defined as the time from treatment until objective tumor progression or death, whichever occurs first.

Full Information

First Posted
June 28, 2021
Last Updated
September 2, 2023
Sponsor
Baylor College of Medicine
Collaborators
The Methodist Hospital Research Institute
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1. Study Identification

Unique Protocol Identification Number
NCT04952584
Brief Title
Allogeneic CD30 Chimeric Antigen Receptor Epstein-Barr Virus-Specific T Lymphocytes in Relapsed or Refractory CD30-Positive Lymphomas
Official Title
A Phase 1 Study Evaluating the Safety and Activity of Allogeneic CD30 Chimeric Antigen Receptor Epstein-Barr Virus-Specific T Lymphocytes (CD30.CAR-EBVSTs) in Patients With Relapsed or Refractory CD30-Positive Lymphomas
Study Type
Interventional

2. Study Status

Record Verification Date
September 2023
Overall Recruitment Status
Withdrawn
Why Stopped
Study was not activated
Study Start Date
March 2024 (Anticipated)
Primary Completion Date
June 2025 (Anticipated)
Study Completion Date
June 2040 (Anticipated)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
Baylor College of Medicine
Collaborators
The Methodist Hospital Research Institute

4. Oversight

Studies a U.S. FDA-regulated Drug Product
Yes
Studies a U.S. FDA-regulated Device Product
No
Data Monitoring Committee
Yes

5. Study Description

Brief Summary
This study involved patients that have a cancer called diffuse large B cell lymphoma (DLBCL), NK and T cell lymphomas (NK/TL) or classical Hodgkin lymphoma (cHL) (hereafter these 3 diseases will be referred to as lymphoma). Patients lymphoma has come back or not gone away after treatment. Because there is no standard treatment for the patients cancer at this time or because the currently used treatments do not work fully in all cases, the patients are being asked to volunteer in this research study. In this study the investigators want to test a type of T cell made from a normal donor. The T cells the investigators will use are called Epstein Barr virus (EBV) specific T cells (EBVSTs) and are cells that the investigators have trained in the laboratory to recognize a EBV which is the virus that causes mono or kissing disease. Some patients with lymphoma have EBV in their cancer cells. Researchers have given T cell lines from normal donor EBVSTs to lymphoma patients who have EBV in their lymphoma cells and have seen responses in about half the patients. The cells have have been generated and are frozen in a bank. The cells are called "allogeneic" (meaning the donor is not related to the patient). CD30.CAR in EBV-specific T cells (called allogeneic CD30.CAR-EBVST) from the blood of healthy donors. The investigators are giving the cells to patients with lymphoma cells that express CD30. If the lymphoma cells also express EBV there may be some benefit from targeting both proteins. The purpose of this study is to find out the highest safe dose of allogeneic CD30.CAR-EBVST cells given following chemotherapy and used to treat lymphoma. The investigators will learn the side effects of CD30.CAR-EBVST cells in patients and see whether this therapy may help lymphoma patients.
Detailed Description
Earlier, healthy donors gave blood for us to make CD30.CAR-EBVST cells in the laboratory. These cells were grown and frozen and the investigators will select the donor which the investigators think is the best match for the patient. This is a dose escalation study. This means that at the beginning, patients will be started on the lowest dose (1 of 3 different levels) of CD30.CAR-EBVST cells. Once the lower dose schedule proves safe, the next group of patients will be started at a higher dose. This process will continue until all 3 dose levels are studied. If the side effects are too severe, the dose will be lowered or the T cell infusion will be stopped. Both the risks and benefits of this study may be dose related. The investigators don't know the best dose that will provide benefit while minimizing the risks. To enroll on this study, patients will need to have recovered from toxic effects of previous chemotherapy and not be receiving any other investigational agents. Patients cannot have received any tumor vaccines within the previous six weeks. If patients agree to take part in this study, the investigators will ask the patients to adhere to the following study visits and procedure. After patients have signed the consent form, patients are required to come to the hospital for a series of standard medical screening tests, lymphodepletion chemotherapy with cyclophosphamide and fludarabine, infusion with CD30.CAR-EBVST cell treatment and follow-up visits (See details below). Screening tests Screening tests include: Blood tests [Human Leukocyte Antigen (HLA) testing] to help us identify the best match for the patient from the banked CD30.CAR-EBVST cells. Blood tests for viruses such as human immunodeficiency virus [HIV], human T cell lymphotropic virus [HTLV], hepatitis B virus and hepatitis C virus. Tumor biopsy test to check the status of CD30. Once the investigators find that patients are eligible for this study, patients will be called for additional screening tests before treatment day. The screening tests include: Physical examination Vital signs tests to measure temperature, pulse, respiratory rate and blood pressure Blood tests to measure blood cells, kidney and liver functions Urine test Pregnancy test for women of child-bearing potential Measurements of tumor by routine imaging studies Lymphodepletion chemotherapy Several studies suggest that the infused T cells need room to be able to multiply and grow to accomplish their functions and that this may not happen if there are too many other T cells in the blood stream. Because of that, if patients have NOT had a bone marrow or stem cell transplant recently, patients will receive treatment with cyclophosphamide and fludarabine (chemotherapy drugs) before patients receive the CD30.CAR-EBVST cells if patients doctor thinks this is appropriate. This is called "lymphodepletion". These drugs will decrease the numbers of patients own T cells before the investigators inject the CD30.CAR-EBVST cells. Although the investigators do not expect any effect on the patients tumor with the dose that the patients will receive, these drugs are part of many regimens that are used to treat lymphoma. Treatment with CD30.CAR-EBVST cells Patients will be given one injection of CD30.CAR-EBVST cells. The CD30.CAR-EBVST cells will be injected into the vein through an IV line at the assigned dose. Before patients receive the injection, patients may be given a dose of acetaminophen or anti-histamine (Benadryl for example) to minimize any possible allergic reaction. The injection of CD30.CAR-EBVST cells will take within 10 minutes. The investigators will follow patients in the clinic after each injection for up to 3 hours. The patient will need to stay less than 2 hours away from the Medical Center for 4 weeks after the CD30.CAR-EBVST cell infusion so the investigator can monitor them for side effects. At the discretion of the study doctor, if patients have stable disease (the lymphoma did not grow) or there is a reduction in the size of the patients lymphoma on imaging studies at week 6 after T-cell infusion or on subsequent evaluations, then patients may receive up to two additional doses of the CD30.CAR-EBVST cells at approximately 6 weeks intervals if patients wish to. Lymphodepletion chemotherapy may also be administered before additional doses of CD30.CAR-EBVST infusion. After each T-cell infusion, patients will be monitored to ensure that participants don't have a reaction to the infusion. Follow-up visits On follow-up visits after treatment, patients will also receive a series of standard medical tests: Physical examination Vital sign tests to measure temperature, pulse, respiratory rate and blood pressure Blood tests to measure blood cells, kidney and liver functions Urine tests (if clinically necessary) Pregnancy test for women of child-bearing potential (if clinically necessary) Measurements of tumor by routine imaging studies After infusion of CD30.CAR-EBVST cells,the patients blood will be collected on follow-up visits at week 1, week 2, week 3, week 4, week 8, every 3 months for 1 year, every 6 months for 4 more years. Blood samples and tumor biopsies will also be periodically collected, based on the patients doctor's discretion for exploratory tests in the laboratory. After 5 years, in the event there is suspected or new cancer is detected, additional blood sample will also be collected for additional tests. To learn more about the way the CD30.CAR-EBVST cells are working and how long they last in the body, the investigators will draw blood. The total amount of blood collected on any day is about 4-18 teaspoons (18-87 ml). This volume is considered safe but may be decreased if the patients are anemic. Patients blood will be drawn from a central line if participants have one. The total blood drawn during the patients participation in this study will not exceed 110 teaspoons (546 ml). The investigators will also look at any scans or biopsies patients have had as standard of care.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Extranodal Natural Killer/T-Cell Lymphoma, Nasal Type, Classical Hodgkin Lymphoma
Keywords
CD30-Positive Lymphoma, Hodgkin lymphoma, Non-Hodgkin lymphoma, CD30 CAR

7. Study Design

Primary Purpose
Treatment
Study Phase
Phase 1
Interventional Study Model
Single Group Assignment
Masking
None (Open Label)
Allocation
N/A
Enrollment
0 (Actual)

8. Arms, Groups, and Interventions

Arm Title
Treatment Phase
Arm Type
Experimental
Arm Description
Three dose levels will be evaluated based on safety data from our current study of CD30 CAR T cells. Cohorts of three to six patients will be enrolled at each dose level The dose is based on the number of CD.30 CAR-EBVT-expressing cells administered. The total number of dose levels evaluated will depend upon toxicities experienced. Dose level cohorts will be numbered sequentially. Dose Level 1: 1 × 10^8 CD30.CAR-EBVST cells Dose Level 2: 4 × 10^8 CD30.CAR-EBVST cells Dose Level 3: 1 × 10^9 CD30.CAR-EBVST cells
Intervention Type
Biological
Intervention Name(s)
CD30.CAR-EBVST cells
Other Intervention Name(s)
Allogeneic CD30 Chimeric Antigen Receptor Epstein-Barr Virus-Specific T Lymphocytes
Intervention Description
The dose is based on the number of CD30.CAR-expressing cells. In our previous study the highest dose was 2 × 10^8 cells/m2 and we did not reach a MTD. There will be a gap of 4 weeks between the first and second patient on each dose level. On Day 0 of study, patients will receive their planned single dose of investigational T cell product by IV infusion over approximately 1 to 10 minutes in an expected volume of 1 to 50 mL.
Primary Outcome Measure Information:
Title
Dose limiting toxicity rate (DLT) by CTCAE 5.0
Description
Any Grade 5 event, / Non-hematologic dose-limiting toxicity is any Grade 3 or Grade 4 non-hematologic toxicity that fails to return to Grade 2 within 72 hours, / Grade 2-4 allergic reaction to T-Cells, / Grade 3-4 GVHD, / Grade 3-4 CRS. Toxicity will be evaluated according to the CTCAE Version 5.0. GVHD will be graded by the method of Przepiorka et al.
Time Frame
28 Days
Secondary Outcome Measure Information:
Title
Rate of Anti-Tumor effect Objective Response (OR)
Description
Objective response rate is defined as complete response and partial response
Time Frame
4 to 12 weeks post CTL infusion
Title
Duration of Response
Description
Response duration will be measured from the time of initial response until documented tumor progression.
Time Frame
Up to 5 years
Title
Stable disease (SD) rate
Description
SD will be defined as the proportion of patients that have stable disease
Time Frame
4 to 12 weeks post CTL infusion
Title
Duration of SD
Description
Stable disease is measured from the start of the treatment until the criteria for progression are met.
Time Frame
Up to 5 years
Title
Progression free survival (PFS)
Description
PFS is defined as the time from treatment until objective tumor progression or death, whichever occurs first.
Time Frame
Up to 5 years

10. Eligibility

Sex
All
Minimum Age & Unit of Time
12 Years
Maximum Age & Unit of Time
75 Years
Accepts Healthy Volunteers
No
Eligibility Criteria
Inclusion Criteria: Diagnosis and clinical course falling into one of the following categories: Hodgkin lymphoma Aggressive non-Hodgkin lymphoma ALK-negative anaplastic T cell lymphoma or other peripheral T-cell lymphoma ALK-positive anaplastic T cell lymphoma CD30-positive tumor as assayed in a CLIA certified Pathology Laboratory. Age 12 to 75. Bilirubin 2 times (or 3 times if the patient has Gilbert syndrome) or less than the upper limit of normal. AST 3 times or less than the upper limit of normal. Estimated GFR > 70 mL/min. Pulse oximetry of > 90% on room air EKG shows no significant arrhythmias Karnofsky or Lansky score of > 60%. Available allogeneic T cells with ≥15% expression of CD30CAR determined by flow-cytometry. Recovered from all acute non-hematologic toxic effects of all prior chemotherapy. Sexually active patients must be willing to utilize one of the more effective birth control methods during the study and for 6 months after the study is concluded. The male partner should use a condom. Informed consent explained to, understood by and signed by patient or guardian. Patient or guardian given a copy of the informed consent form. Exclusion Criteria: Currently receiving any investigational agents or received any tumor vaccines within the previous six weeks. Received CD30 antibody-based therapy within the previous 4 weeks. History of hypersensitivity reactions to murine protein-containing products. Pregnant or lactating. Tumor in a location where enlargement could cause airway obstruction. Current use of systemic corticosteroids at a dose equivalent to higher than 10 mg/day of prednisone. Active significant, uncontrolled bacterial, viral or fungal infection. Symptomatic cardiac disease (NYHA Class III or IV disease).
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Premal Lulla, MD
Organizational Affiliation
Baylor College of Medicine
Official's Role
Principal Investigator
Facility Information:
Facility Name
Houston Methodist Hospital
City
Houston
State/Province
Texas
ZIP/Postal Code
77030
Country
United States
Facility Name
Texas Children's Hospital
City
Houston
State/Province
Texas
ZIP/Postal Code
77030
Country
United States

12. IPD Sharing Statement

Learn more about this trial

Allogeneic CD30 Chimeric Antigen Receptor Epstein-Barr Virus-Specific T Lymphocytes in Relapsed or Refractory CD30-Positive Lymphomas

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