Repertoire and Properties of Anti-drug Antibodies Involved in Immediate Hypersensitivity in the Operating Room (MEDIREP)
Hypersensitivity, Immediate, Peri-operative Injury, Antibiotic Allergy
About this trial
This is an interventional basic science trial for Hypersensitivity, Immediate focused on measuring Hypersensitivity, IgE, IgG, repertoire, NMBA, anaphylaxis
Eligibility Criteria
Inclusion Criteria:
Common to both groups:
- Age ≥ 18 years old and ≤ 70 years old
- Patient having presented an allergic reaction in the operating room in the last 10 years, regardless of the grade of severity, the causative agent or the type of anesthesia
- Patient having benefited from an allergy-anaesthesia consultation in the investigator center
For IgE group:
- presence of at least one positive skin test to one of the suspected agent (curare and/or antibiotic) during the allergist consultation
For the IgG group:
- presence of circulating IgG-type antibodies directed against one of the suspected agents (curare, antibiotic, antiseptic), identified in the routine biological tests carried out at the time of the shock and / or the consultation of allergology.
Exclusion Criteria:
Common to both groups:
- Absence of written informed consent
- Protected person: under guardianship or curatorship
- Patient without social security
- Pregnancy of breast feeding
- Ongoing immunosuppressive or chemotherapy
- Acute heart failure
- Patient included in another interventional research with an exclusion period
For IgE group:
- Taking oral or injectable anticoagulants
- Taking a double antiplatelet aggregation
- Previous sternotomy
- Previous thoracic radiation therapy
- Known allergy to local anesthesic or ioda
- Previous major sternal cutaneous lesions
Prerequisites for carrying out the sternal sample in order to carry out a myelogram (IgE group):
- Leukocytes > 4 giga/L in the 2 months preceding inclusion
- Platelets > 100,000 / mm3 in the 2 months preceding inclusion
For the IgG group
- Orthostatic hypotension
- Realization of a blood donation in the 6 to 8 weeks preceding inclusion
Prerequisites for carrying out the sternal sample in order to carry out a myelogram (IgE group):
- Leukocytes > 4 giga/L in the 2 months preceding inclusion
- Platelets > 100,000 / mm3 in the 2 months preceding inclusion
Prerequisites for performing the blood sample (IgG group):
- Hemoglobin > 12.5 g/dL for women and > 13 g/dL for men in the 2 months preceding inclusion
Sites / Locations
- Hôpital Bichat claude BernardRecruiting
Arms of the Study
Arm 1
Arm 2
Arm 3
Other
Other
Other
IgG group
IgE group
IgG group + IgE group
Patient in the IgG group will benefit from a blood sampling of 5 ml (for realization of a basophil activation test) and a blood sampling of 250 ml
Patient in the IgE will benefit from a blood sampling of 5 ml (for realization of a basophil activation test) and a bone marrow sampling (approx 2 ml)
Patient in the IgG + IgE will benefit from a blood sampling of 5 ml (for realization of a basophil activation test), a blood sampling of 250 ml and a bone marrow sampling (approx 2 ml)