Physical performance
Participants will perform a time trial to assess physical performance. Participants will be able to start a 3 min fixed intensity warm-up. The trial itself lasts 15 min and participants are instructed to cover as much distance as possible. To keep the influence of motivation to a minimum, no encouragements or performance feedback will be given during the trial. The participants will only see the amount of time they have left on a digital chronometer. After the trial, a 2 min self-paced cooldown period will start. The evaluted outcome will be total workload performed by the participants (in kJ).
Cognitive performance: Go NoGo task: attention
Cognitive performance will be assessed using a task based on the go/no-go paradigm. Participants are to react to different stimuli displayed on a computer screen positioned about 1 meter from the subject, preceded by a screen with general instructions. Two different stimuli were present at the same time: a Go or NoGo stimuli, and a left or right stimuli (meaning that four combinations are possible: GoRight, GoLeft, NoGoRight and NoGoLeft). If a Go stimuli is presented, participants are instructed to react to the left or right stimuli with the corresponding arrows. However, if a NoGo stimulus is presented, participants are instructed to refrain from reacting to the either the left or right stimuli. This proposed paradigm measures attention, response inhibition and working memory. Stimuli will be presented for 500 ms, with a varying interstimulus time between 1100 and 1700 ms. Outcome for attention will be reaction time on the Go trials.
Cognitive performance: Go NoGo task: response inhibition
Cognitive performance will be assessed using a task based on the go/no-go paradigm. Participants are to react to different stimuli displayed on a computer screen positioned about 1 meter from the subject, preceded by a screen with general instructions. Two different stimuli were present at the same time: a Go or NoGo stimuli, and a left or right stimuli (meaning that four combinations are possible: GoRight, GoLeft, NoGoRight and NoGoLeft). If a Go stimuli is presented, participants are instructed to react to the left or right stimuli with the corresponding arrows. However, if a NoGo stimulus is presented, participants are instructed to refrain from reacting to the either the left or right stimuli. This proposed paradigm measures attention, response inhibition and working memory. Stimuli will be presented for 500 ms, with a varying interstimulus time between 1100 and 1700 ms. Outcome for response inhibition will be accuracy on the NoGo trials.
Subjective Manipulation check: M-VAS
The M-VAS-scale (0-10cm) will be taken at multiple different time points (i.e. at the start of the trial, after the EEG baseline, after the pre cognitive performance task, after the behavioural manipulation check, during the intervention/control task, after the post behavioural manipulation check, after the post cognitive performance outcome and after the physical task). It will assess how mentally fatigued the subject is feeling (100 point VAS scale), and ranges from 'not at all' to 'completely exhausted'. The validity and reliability of a visual analogue scale to assess general fatigue was demonstrated by Lee et al., and this type of scale has been shown to be one of the most sensitive assessments of MF.
Behavioural Manipulation check: Stroop reaction time
Reaction time on the stroop will be assessed to determine behavioural induction of mental fatigue.
Behavioural Manipulation check: Stroop accuracy
Accuracy on the stroop will be assessed to determine behavioural induction of mental fatigue.
Physiological manipulation check - EEG (theta and alpha power and ERPs)
Electroencephalography (EEG) will be used to continuously measure brain activity by using 32 active Ag/AgCl electrodes attached on the participants' head (Acticap, Brain Products Munich, Germany), according to the "10:20 International System". The sampling rate will be set at 500 Hz (Brain Vision Recorder, Brain Products, Munich, Germany). Electrode impedance will be kept <10 kΩ throughout the recording. Baseline measurements will be taken 2 min with eyes open, 2 min with eyes closed and subjects will be seated in a sound isolated dimly lit room. Afterwards, Event-related potentials (ERP), spectral power and brain connectivity analysis will be executed. The aim of the EEG device is to measure the presence of MF during the Stroop task, and to identify possible mechanisms behind the suspected decreases in human performance.
Age
Age will be assessed to determine influences on mental fatigue effects
Length
Length will be assessed to determine influences on mental fatigue effects (using SECA 206, SECA Benelux, The Netherlands)
Weight
Weight will be assessed to determine influences on mental fatigue effects (using Tanita TBF 300, Tanita Europe BV, The Netherlands)
Sex
Sex will be assessed to determine influences on mental fatigue effects
Fat percentage
Fat percentage will be assessed to determine influences on mental fatigue effects (Tanita TBF 300, Tanita Europe BV, The Netherlands)
Burn out
General health will be assessed using the Burn out assessment tool (BAT). The questionnaire assesses 4 core domains in burn out: exhaustion, emotional impairment, cognitive impairment and mental distance Moreover, 2 additional domains that assess specific symptoms of burn out are also assessed: psychosomatic complaints and psychological distress.
Recovery need
Additionally, a questionnaire that determines recovery need will be added to the burn out assessment. For this part, we will use the recovery need section of the "Short Inventory to Monitor Psychosocial Hazards" questionnaire.
Physical training level (objective)
Objectively, a graded maximal cycle ergometer test will be performed in 18-20°C and a RH of 40%, to attain the maximal exercise ability. This ability will then be translated to a specific performance level as defined by De Pauw et al.
Physical training level (subjective)
Subjectively, the international physical activity questionnaire short form (IPAQ-SF) will assess the overall physical activity habits of the participant. The IPAQ-SF consists of 7 questions, assessing amount and level of intensity of physical activity in the last 7 days, and represents good reliability and validity.
Sport participation
a specific questionnaire will be designed with questions examining sport participation. These answers on these questions will then be employed to divide participants into a tier based system as proposed by McKay et al.
Working memory
Working memory will be assessed using a short n-back task. Participants will be instructed to focus on a white computer screen with a randomly generated number between 0-9. They have to press a button if the number in front of them matches the number they encountered 2 numbers earlier (2 back paradigm). Stimuli are presented for 500 ms, with an interstimulus time of 2500 ms, and a target/non target ratio of 33/67 (58). Outcomes include ACC (percentage of correct responses).
Response inhibition
To examine response inhibition, participants will be required to perform a sustained attention to response task (SART). Participants were presented with single digits in the centre of the screen, ranging from 0-9, and were instructed to react as fast as possible if a number was shown on screen. However, if the number that was displayed equalled 3, participants were asked to refrain from responding. To specifically measure response inhibition, the outcome of this task consisted of the percentage of correct responses to the number 3 stimulus.
Attention
Attention will be assessed using a psychomotor vigilance task (PVT). The task features a red triangle in the centre of a computer screen, where participants are required to react as fast as possible when a yellow count is started in the middle of this red triangle. The interstimulus time ranges between 2 and 10 sec. Mean RT is collected as outcome, and false alarms (>200 ms reaction) and misses (>900 ms) are also measured.
Genetics
Genes will be investigated using blood samples which will be captured in EDTA tubes and stored at -80 °C at the lab. The blood samples will be taken in between the physical assessment and the maximal incremental exercise test. One sample will then be sent to BRIGHTcore where the DNA will be extracted and analysed using exome sequencing.
Sleep quality
Sleep quality will be assessed using the Pittsburgh Sleep Quality Index (PSQI). The PSQI's 19 self-reported items belong to one of seven subcategories: subjective sleep quality, sleep latency, sleep duration, habitual sleep efficiency, sleep disturbances, use of sleeping medication, and daytime dysfunction. The initial evaluation of the index found a reliability of 0.83 (good), and a test-retest reliability of 0.85 (good). Overall, the PSQI shows excellent reliability and validity for both clinical as well as non-clinical samples.
Chronotype
Sleep profile will be assessed using the single item chronotype scale (SIC). The SIC features six images relating to different chronotypes, and participants are required to select the picture that most closely relates to their perceived chronotype ("Which image best corresponds to your activation level during the day (i.e. in the morning, during daytime/afternoon, in the evening)?").
Trait Self control
Trait self-control will be assessed using brief self-control scale developed by Tangney et al.. This 13 item scale is designed to assess trait self-control and has been shown to be correlated with the total self-control scale. It supports questions such as "I am good at resisting temptation" where participants are instructed to indicate if this statement is "not at all" (1) to "very much" (5) applicable to themselves.
Trait Anxiety
The state-trait anxiety inventory involves two 20-item subscales describing trait and state anxiety, respectively. Only the first subscale will be used in this study. A score between 1 and 4 (from "Not at all "to "Very much so ") has to be provided on every item. The higher the score, the more severe the trait anxiety.
Mental Toughness
The eight item mental toughness questionnaire designed by Gucciardi et al. will be used asses MT. Participants will be asked to indicate how true each of the statements are (e.g., "I strive for continued success"), using a seven-point Likert scale (1-7) where 1 is designated as "false, 100% of the time" and 7 is designated as "true, 100% of the time".
Caffeine use
Will be determined using the revised caffeine consumption questionnaire (CCQ-R). This questionnaire is designed to assess the approximate self-reported consumption of caffeine during the week, with products ranging from coffee and tea to food containing caffeine and drugs, and takes about 5 min to complete. The CCQ-R is a valid way to assess caffein consumption.