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Stimulation Sites and Fatigue Induced by Neuromuscular Electrical Stimulation in Healthy Individuals

Primary Purpose

Fatigue

Status
Recruiting
Phase
Not Applicable
Locations
Brazil
Study Type
Interventional
Intervention
Electric stimulation
Sponsored by
Marco Aurélio Vaz, PhD
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional basic science trial for Fatigue focused on measuring Electrical stimulation, Fatigue

Eligibility Criteria

18 Years - 40 Years (Adult)All SexesAccepts Healthy Volunteers

Inclusion Criteria: Healthy individuals: young people of both sexes age between 18 and 40 years body mass index between 20 and 25 kg/m2 and normal knee range of motion with no complaints of pain or presence of pathology in the dominant lower limb. - Exclusion Criteria: Individuals with: any contraindication to maximal exercise having been treated with NMES in the last 3 months in the lower limb not tolerate electrical stimulation

Sites / Locations

  • Exercise Research Laboratory, School of Physical Education, Federal University of Rio Grande do SulRecruiting

Arms of the Study

Arm 1

Arm 2

Arm 3

Arm 4

Arm 5

Arm 6

Arm Type

Experimental

Experimental

Experimental

Experimental

Experimental

Experimental

Arm Label

FN-NMES - 20 Hz

FN-NMES - 100 Hz

MP-NMES - 20 Hz

MP-NMES - 100 Hz

FNMP-NMES - 20 Hz

FNMP-NMES - 100 Hz

Arm Description

This group will receive a NMES-induced fatigue protocol, which will be applied to the femoral nerve (FN) using a stimulation frequency of 20 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee in 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contraction.

This group will receive a NMES-induced fatigue protocol, which will be applied to the femoral nerve (FN) using a stimulation frequency of 100 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee in 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.

This arm will receive a NMES-induced fatigue protocol, which will be applied to the motor point (MP) of the rectus femoris muscle using a stimulation frequency of 20 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee at 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.

This arm will receive a NMES-induced fatigue protocol, which will be applied to the motor point (MP) of the rectus femoris muscle using a stimulation frequency of 100 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee at 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.

This group will receive a NMES-induced fatigue protocol, which will be applied to the femoral nerve (FN) and to the motor point (MP) of the rectus femoris muscle using a stimulation frequency of 20 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee at 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.

This group will receive a NMES-induced fatigue protocol, which will be applied to the femoral nerve (FN) and to the motor point (MP) of the rectus femoris muscle using a stimulation frequency of 100 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee at 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.

Outcomes

Primary Outcome Measures

Level of physical activity
The level of physical activity (PA) of each subject will be assessed by the International Physical Activity Questionnaire (IPAQ).The scores will be assessed by calculating the metabolic equivalents (MET) for each activity level. Walking score will be achieved by the multiplication of 3.3 METs with the total walking duration in minutes in a week. Moderate physical activity scores will be achieved by the multiplication of 4.0 METs with the total moderate physical activity duration in minutes in a week. Vigorous physical activity scores will be achieved by the multiplication of 8.0 METs with the total vigorous physical activity duration in minutes in a week. Total physical activity MET-minutes/week will be obtained through sum of walking, moderate and vigorous MET minutes/week scores. Categorical Score will be classified into three levels of physical activity: low, moderate and high.
Thickness of the subcutaneous fat layer on the motor point and femoral nerve
Corresponds to the amount of subcutaneous adipose tissue and will be evaluated by ultrasonography
Maximum voluntary isometric contraction of the knee extensors
Knee extensor maximal voluntary isometric contraction (MVIC) is an expression of the muscular strength, and will be evaluated by dynamometry.
Current intensity required to evoke knee extensors' submaximal torque (20% MVIC)
The amount or amplitude of electrical current (in milliamperes - mA) required to achieve a specific force (20% MVIC), and will be evaluated from the electrical stimulation device.
Change from rest Evoked torque by supramaximal twitch at pre-intervention and immediately after the intervention
Simple biphasic rectangular electrical pulses will be applied at a frequency of 1 Hz at a current intensity sufficient to generate a visible contraction of the knee extensors. From the identification of this motor threshold, the current intensity will be gradually increased until no subsequent increase in the evoked twitch torque is observed. Then, the current intensity will be increased by 10% in order to guarantee that the torque evoked by the twitch is supramaximal. The torque (Nm) evoked during the twitch will be determined by the torque produced by three supramaximal stimuli.
Muscle Fatigue Index
Characterized by the maximal voluntary isometric contraction decrease after the fatigue protocol, it will be evaluated by dynamometry.
Fatigue Index from Evoked Torque
Characterized by the decrease of the evoked torque during the fatigue protocol, the fatigue index from the evoked torque will be evaluated by dynamometry, and obtained by the analysis of evoked torque curves
Reduction of the torque evoked until reaching 50% of the torque evoked by the first contraction
It will be obtained from the analysis of the evoked contractions' torque-time curves. The torque evoked by each contraction will be normalized by the torque evoked by the first contraction (which will correspond to 100%). The number of contractions until the relative evoked torque to undergo a 50% reduction in relation to the evoked torque of the initial contraction will be used for analysis.
Total work generated during the fatigue protocol
The torque-time integral of the evoked torque curves during the fatigue protocol will be evaluated and correspond to the mechanical work of each evoked contraction. The sum of the torque curve integral of all evoked contractions during the fatigue protocol will be calculated to determine the total work evoked by each current during the fatigue protocol.
Discomfort level generated by electrical stimulation during the fatigue protocol
Discomfort will be measured with a Visual Analogue Scale (0-100mm), where 0 and 100 mm correspond to no discomfort and worst perceived discomfort, respectively

Secondary Outcome Measures

Full Information

First Posted
October 6, 2022
Last Updated
March 2, 2023
Sponsor
Marco Aurélio Vaz, PhD
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1. Study Identification

Unique Protocol Identification Number
NCT05605210
Brief Title
Stimulation Sites and Fatigue Induced by Neuromuscular Electrical Stimulation in Healthy Individuals
Official Title
Stimulation Sites and Fatigue Induced by Neuromuscular Electrical Stimulation in Healthy Individuals
Study Type
Interventional

2. Study Status

Record Verification Date
March 2023
Overall Recruitment Status
Recruiting
Study Start Date
January 25, 2023 (Actual)
Primary Completion Date
December 1, 2023 (Anticipated)
Study Completion Date
August 1, 2024 (Anticipated)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Sponsor-Investigator
Name of the Sponsor
Marco Aurélio Vaz, PhD

4. Oversight

Studies a U.S. FDA-regulated Drug Product
No
Studies a U.S. FDA-regulated Device Product
No
Data Monitoring Committee
Yes

5. Study Description

Brief Summary
Neuromuscular electrical stimulation (NMES) is a tool used in training protocols and in clinical practice to prevent or attenuate atrophy and improve the ability to produce muscle strength in different populations. Although widely used, the effects of NMES can be limited by discomfort and early fatigue induced by electrical current. Previous studies have investigated alternatives to minimize muscle fatigue, reduce muscle discomfort and increase muscle performance. A measure adopted to reduce the NMES's deleterious effects is the choice of stimulation site. More specifically, muscle contractions can be evoked by applying electrical pulses to the trunk of peripheral nerves (nNMES) or terminal branches of the nerve at the muscle belly level (mNMES). There is evidence that the mNMES stimulates the more superficial motor units (MUs), while the deeper MUs of the muscle remain inactivated, or, to recruit them, an additional increase in current intensity and stimulation frequency may be required. On the other hand, in direct nerve stimulation (nNMES) both superficial and deep MUs are recruited regardless of NMES intensity. Based on these observations, a new application modality of NMES emerged, the intercalated nerve and muscle stimulation (iNMES). In this strategy, electrical pulses are intercalated or alternated between the mNMES and nNMES sites, intending to reduce the high frequencies at which the MUs are activated during NMES, recruiting both superficial and deep MUs, and reducing muscle fatigue during evoked contractions. Although iEENM is a promising strategy to potentiate the NMES effects, few studies have investigated the iNMES effects on neuromuscular fatigue, and the existing literature is solely focused on the analysis of the tibialis anterior muscle, limiting the findings' inferences for other muscles important for lower limb functionality (e.g., quadriceps femoris). Therefore, the objective of this study is to compare the effects of nNMES applied to the femoral nerve (FN-NMES), of mNMES applied to the rectus femoris' motor point (MP-NMES), and iNMES applied simultaneously to both sites (FNMP-NMES) on knee extensors' functional (muscle fatigue) and clinical (discomfort) parameters in healthy individuals, through a randomized clinical trial. Our study has three hypotheses. In our first hypothesis, muscle fatigue during an electrical stimulation protocol will be lower with the FNMP-NMES modality, followed by FN-NMES, and will be higher with MP-NMES. Thus, FNMP-NMES will present a smaller reduction in maximal voluntary isometric contractions (MVICs) immediately after the fatigue protocol, a smaller relative reduction between the final compared to the initial evoked torque, a greater number of contractions for the evoked torque to reduce 50% with respect to the initial torque during the NMES fatigue protocol, and a greater total work compared the FN-NMES and MP-NMES modalities. In our second hypothesis, low frequency (20 Hz) NMES will produce greater total work and less fatigability of the knee extensors (smaller reduction from pre to post MVIC, smaller percentage reduction at the final compared to the initial evoked torque, a greater number of contractions for the evoked torque to reduce 50% compared to the initial evoked torque, and greater total work) compared to a high stimulation frequency (100 Hz). Furthermore, the total work will be higher and the fatigability lower with FNMP-NMES, followed by FN-NMES, and finally MP-NMES, regardless of stimulation frequency. Finally, the third hypothesis is that discomfort will be less with FNMP-NMES, followed by FN-NMES, and finally MP-NMES, regardless of stimulation frequency.
Detailed Description
This project will be developed through one randomized clinical study, which will be designed to compare the effects of nNMES applied to the femoral nerve (NF-NMES), mNMES applied to the motor point (PM-NMES), and iEENM applied simultaneously at both sites (NFPM-NMES), using low (20Hz) and high (100 Hz) stimulation frequency, on knee extensors' fatigability and discomfort in healthy subjects. Therefore, this study is characterized by a quantitative approach with a randomized clinical cross-over design, blinded to evaluators and participants. The sample will be characterized by convenience, and healthy men and women (self-declaration that they do not have diseases), aged between 18 and 40 years, will be recruited. To define the sample size, the G-Power software (version 3.1.3; University of Trier, Trier, Germany) was used, and a significance level was adopted for α = 0.05 and power (1-β) = 0.80, for all calculated variables. As the fatigability variable demonstrated the need for a larger sample (24 participants) to avoid errors and reach a minimum statistical power of 80%, the investigators will use the result of this variable for the present study. However, a previous study (DANTAS et al., 2015) reported the exclusion of 25% of individuals who were recruited for evaluation, for reasons related to NMES. Therefore, considering possible exclusions or even losses throughout the protocol, the investigators will recruit 30 participants throughout the study, 15 men and 15 women. Assessments will be carried out on 6 different days for each participant. The duration of each evaluation will have an average time of 2 hours for the first evaluation day, and 1.5 hours for the other evaluation days. Additionally, women will be evaluated between the 4th and 22nd day of the menstrual cycle, a period in which there is a greater tolerance to NMES. On the first day, anamnesis will be carried out, explanations about the study will be provided and the participant will be familiarized with the assessment techniques and the assessment protocol. After this initial part, an interval of 10 minutes will be observed, and, then, the envelope will be opened containing which application protocol was randomized to be investigated, that is, tests using FN-NMES, MP-NMES, or FNMP-NMES, after which the following steps/tests will be applied: Preparing participants for the tests; Assessment of the supramaximal evoked twitch torque at rest; Assessment of the maximum torque generated during maximal voluntary isometric contraction (MVIC) pre-fatigue; Assessment of the supramaximal evoked twitch torque pre-fatigue; Evaluation of the current intensity to generate a torque at 20% of the MVIC; Application of the NMES-evoked fatigue protocol; Assessment of discomfort during the fatigue protocol; Evaluation of the maximum torque generated during the MVIC after fatigue; Assessment of the supramaximal evoked twitch torque after the fatigue protocol. This study will adopt an intention-to-treat approach. Therefore, the individual who does not attend the scheduled evaluation will be rescheduled for a new evaluation, allowing the individual to carry out all scheduled evaluations. If the subject chooses to withdraw from participating in the research project, the data collected until the end of his/her participation in the study will be used for analysis. Thus, a flowchart will be used to indicate the abandonment of subjects during all phases of the study, if it does occur. In addition, the flowchart will record how individuals will be included/excluded in the different phases of the study. The statistical procedures will be performed in the SPSS 21.0 program for Windows, and the data tabulation in the Excel 2016 program. The distribution of variables will be presented as mean and standard deviation. The Shapiro-Wilk test will be used to analyze the normality of the data distribution. To compare the fatigability between NMES modalities, ANOVA will be used for repeated measures of two intramodality factors (NMES frequencies and pre/post-NMES protocol time) and an intermodality factor (gender). To compare discomfort between NMES modalities, ANOVA will be used for repeated measures of two intramodality factors (NMES frequencies and NMES site) and an intermodality factor (gender). If there is an interaction between factors, a one-way ANOVA for repeated measures will be used to see if there are differences between the modalities, and, to locate the differences, the Bonferroni post hoc test will be used. In addition, the Cohen's "d" effect size will be calculated, which will be categorized as trivial (<0.20), small (0.20-0.49), moderate (0.50-0.79), large (0.80 to 1.29), and very large. (>1.30) (ROSENTHAL, 1996).

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Fatigue
Keywords
Electrical stimulation, Fatigue

7. Study Design

Primary Purpose
Basic Science
Study Phase
Not Applicable
Interventional Study Model
Crossover Assignment
Masking
ParticipantCare ProviderInvestigatorOutcomes Assessor
Allocation
Randomized
Enrollment
30 (Anticipated)

8. Arms, Groups, and Interventions

Arm Title
FN-NMES - 20 Hz
Arm Type
Experimental
Arm Description
This group will receive a NMES-induced fatigue protocol, which will be applied to the femoral nerve (FN) using a stimulation frequency of 20 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee in 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contraction.
Arm Title
FN-NMES - 100 Hz
Arm Type
Experimental
Arm Description
This group will receive a NMES-induced fatigue protocol, which will be applied to the femoral nerve (FN) using a stimulation frequency of 100 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee in 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.
Arm Title
MP-NMES - 20 Hz
Arm Type
Experimental
Arm Description
This arm will receive a NMES-induced fatigue protocol, which will be applied to the motor point (MP) of the rectus femoris muscle using a stimulation frequency of 20 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee at 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.
Arm Title
MP-NMES - 100 Hz
Arm Type
Experimental
Arm Description
This arm will receive a NMES-induced fatigue protocol, which will be applied to the motor point (MP) of the rectus femoris muscle using a stimulation frequency of 100 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee at 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.
Arm Title
FNMP-NMES - 20 Hz
Arm Type
Experimental
Arm Description
This group will receive a NMES-induced fatigue protocol, which will be applied to the femoral nerve (FN) and to the motor point (MP) of the rectus femoris muscle using a stimulation frequency of 20 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee at 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.
Arm Title
FNMP-NMES - 100 Hz
Arm Type
Experimental
Arm Description
This group will receive a NMES-induced fatigue protocol, which will be applied to the femoral nerve (FN) and to the motor point (MP) of the rectus femoris muscle using a stimulation frequency of 100 Hz. For this, the participant will be positioned on the isokinetic dynamometer (Biodex Medical System, Shirley - NY, USA) with the knee at 90° of flexion (0°=total extension). The participant will be instructed to remain relaxed and to not perform active muscle contractions.
Intervention Type
Device
Intervention Name(s)
Electric stimulation
Intervention Description
Neuromuscular electrical stimulation will be applied at two sites to induce fatigue with stimulation frequency specified at the Arm/Group Descriptions. The electrical stimulation parameters to generate the fatigue protocol will be: biphasic pulsed current, pulse duration of 2 ms, and on:off times of 5:10s. The fatigue protocol will be applied with a current intensity sufficient to generate an evoked torque at a level of 20% of the MVIC, which will be maintained throughout the protocol. NMES will be maintained until reaching an intervention time of 20 minutes, generating 80 evoked contractions.
Primary Outcome Measure Information:
Title
Level of physical activity
Description
The level of physical activity (PA) of each subject will be assessed by the International Physical Activity Questionnaire (IPAQ).The scores will be assessed by calculating the metabolic equivalents (MET) for each activity level. Walking score will be achieved by the multiplication of 3.3 METs with the total walking duration in minutes in a week. Moderate physical activity scores will be achieved by the multiplication of 4.0 METs with the total moderate physical activity duration in minutes in a week. Vigorous physical activity scores will be achieved by the multiplication of 8.0 METs with the total vigorous physical activity duration in minutes in a week. Total physical activity MET-minutes/week will be obtained through sum of walking, moderate and vigorous MET minutes/week scores. Categorical Score will be classified into three levels of physical activity: low, moderate and high.
Time Frame
The assessment will be performed before the introduction of the intervention.
Title
Thickness of the subcutaneous fat layer on the motor point and femoral nerve
Description
Corresponds to the amount of subcutaneous adipose tissue and will be evaluated by ultrasonography
Time Frame
The assessment will be performed pre-intervention, that is, before each NMES protocol
Title
Maximum voluntary isometric contraction of the knee extensors
Description
Knee extensor maximal voluntary isometric contraction (MVIC) is an expression of the muscular strength, and will be evaluated by dynamometry.
Time Frame
The assessment will be performed pre-intervention, that is, before each NMES protocol
Title
Current intensity required to evoke knee extensors' submaximal torque (20% MVIC)
Description
The amount or amplitude of electrical current (in milliamperes - mA) required to achieve a specific force (20% MVIC), and will be evaluated from the electrical stimulation device.
Time Frame
The assessment will be performed pre-intervention, that is, before each NMES protocol
Title
Change from rest Evoked torque by supramaximal twitch at pre-intervention and immediately after the intervention
Description
Simple biphasic rectangular electrical pulses will be applied at a frequency of 1 Hz at a current intensity sufficient to generate a visible contraction of the knee extensors. From the identification of this motor threshold, the current intensity will be gradually increased until no subsequent increase in the evoked twitch torque is observed. Then, the current intensity will be increased by 10% in order to guarantee that the torque evoked by the twitch is supramaximal. The torque (Nm) evoked during the twitch will be determined by the torque produced by three supramaximal stimuli.
Time Frame
This evaluation will be carried out at rest, pre-intervention and immediately after the intervention
Title
Muscle Fatigue Index
Description
Characterized by the maximal voluntary isometric contraction decrease after the fatigue protocol, it will be evaluated by dynamometry.
Time Frame
During 5 sec minutes before and after of electrical stimulation protocol
Title
Fatigue Index from Evoked Torque
Description
Characterized by the decrease of the evoked torque during the fatigue protocol, the fatigue index from the evoked torque will be evaluated by dynamometry, and obtained by the analysis of evoked torque curves
Time Frame
During 20 minutes of electrical stimulation
Title
Reduction of the torque evoked until reaching 50% of the torque evoked by the first contraction
Description
It will be obtained from the analysis of the evoked contractions' torque-time curves. The torque evoked by each contraction will be normalized by the torque evoked by the first contraction (which will correspond to 100%). The number of contractions until the relative evoked torque to undergo a 50% reduction in relation to the evoked torque of the initial contraction will be used for analysis.
Time Frame
During 20 minutes of electrical stimulation
Title
Total work generated during the fatigue protocol
Description
The torque-time integral of the evoked torque curves during the fatigue protocol will be evaluated and correspond to the mechanical work of each evoked contraction. The sum of the torque curve integral of all evoked contractions during the fatigue protocol will be calculated to determine the total work evoked by each current during the fatigue protocol.
Time Frame
During 20 minutes of electrical stimulation
Title
Discomfort level generated by electrical stimulation during the fatigue protocol
Description
Discomfort will be measured with a Visual Analogue Scale (0-100mm), where 0 and 100 mm correspond to no discomfort and worst perceived discomfort, respectively
Time Frame
During 20 minutes of electrical stimulation

10. Eligibility

Sex
All
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
40 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria: Healthy individuals: young people of both sexes age between 18 and 40 years body mass index between 20 and 25 kg/m2 and normal knee range of motion with no complaints of pain or presence of pathology in the dominant lower limb. - Exclusion Criteria: Individuals with: any contraindication to maximal exercise having been treated with NMES in the last 3 months in the lower limb not tolerate electrical stimulation
Central Contact Person:
First Name & Middle Initial & Last Name or Official Title & Degree
Marco A Vaz, PhD
Phone
+55 5133085853
Email
marco.vaz@ufrgs.br
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Marco A Vaz, PhD
Organizational Affiliation
Federal University of Rio Grande do Sul
Official's Role
Principal Investigator
First Name & Middle Initial & Last Name & Degree
Marco A Vaz, PhD
Organizational Affiliation
Federal University of Rio Grande do Sul
Official's Role
Study Chair
First Name & Middle Initial & Last Name & Degree
Marco A Vaz, PhD
Organizational Affiliation
Federal University of Rio Grande do Sul
Official's Role
Study Director
Facility Information:
Facility Name
Exercise Research Laboratory, School of Physical Education, Federal University of Rio Grande do Sul
City
Porto Alegre
State/Province
RS
ZIP/Postal Code
90690-200
Country
Brazil
Individual Site Status
Recruiting
Facility Contact:
First Name & Middle Initial & Last Name & Degree
Marco A Vaz, PhD
Phone
+555133085853
Email
marco.vaz@ufrgs.br

12. IPD Sharing Statement

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Stimulation Sites and Fatigue Induced by Neuromuscular Electrical Stimulation in Healthy Individuals

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