FOS Immunohistochemical Staining of Colorectal Cancer and Its Adjacent Tissues
Colorectal Cancer, Monkey Pox
About this trial
This is an interventional diagnostic trial for Colorectal Cancer focused on measuring Differential expression of gene, Immunohistochemical staining, Tumor microenvironment
Eligibility Criteria
Inclusion Criteria: (a) patients with histologically confirmed CRC (b) patients who underwent resection of the primary tumor (c) patients at first visit who had not received treatment before blood testing. Exclusion Criteria: (a) familial adenomatous polyposis or hereditary colon cancer (b) no signs of clinical infection, such as fever, on the day of blood collection; And (c) patients with multiple primary tumors during hospitalization.
Sites / Locations
- Guangxi Medical University Cancer HospitalRecruiting
Arms of the Study
Arm 1
Experimental
Test group-stained by FOS antibody
Sixty colorectal cancer tissue sections were included. The tissue was cut to 5 μm thick and placed on a glass slide for staining. Endogenous peroxidase activity was inhibited and blocked by deparaffin, rehydration, and treatment with 5% bovine serum albumin for 30 min at 37 ° C. The treated sections were incubated with anti-FOS (promab 30360) overnight at 4 ° C and washed three times with PBS. After that, it was required to incubate with secondary anti-peroxidation sunflower for 30 min at 37 ° C. After washing three times with PBS, the images were developed in diamine benzidine and micrographs were obtained under a light microscope.