Outcome of Using Sperm Bound to the Zona Pellucida of Immature Oocytes for Intracytoplasmic Sperm Injection (ICSI)
Intracytoplasmic Sperm Injection, Infertility, Male
About this trial
This is an interventional treatment trial for Intracytoplasmic Sperm Injection focused on measuring Intracytoplasmic sperm injection (ICSI), zona pellucida (ZP), natural sperm selection, Sperm-oocyte interaction, ZP-bound sperm
Eligibility Criteria
Inclusion Criteria: Couples must be diagnosed with infertility and undergoing ICSI cycle. The age range for all female subjects must be between 21 and 38 years old, and their male partners must be older than 24 years old and above 50 years old. Patients must produce at least two mature oocytes (one to be used for control and one for treatment) and one immature oocyte (to be used for selecting sperm). Males must show at least 10% total sperm motility Exclusion Criteria: Cases where the female was younger than 21 years or older than 38 years old. Patients that had less than two mature oocytes Patients with no immature oocytes Patients with low oocyte quality. Cases with less than 10% motility or those with no motility such as PESA, TESA, and TESE cases.
Sites / Locations
- Al-Azhar University
Arms of the Study
Arm 1
Arm 2
No Intervention
Experimental
control
treatment
Oocytes of the control group were injected with conventionally selected spermatozoa based on sperm morphology and motility after being processed density gradient centrifugation
ZP-bound sperm were selected from the surface of the immature oocytes through the use of a microneedle (Sunlight Medical, Jacksonville, FL, USA) and transferred in a 10 % polyvinylpyrrolidone (PVP) solution (SAGE, USA), immobilized, and then used to inject sibling MII oocytes