Normothermic Machine Perfusion (NMP) Versus Hypothermic Machine Perfusion (HMP) in Human Kidney Transplantation
Kidney Transplantation
About this trial
This is an interventional treatment trial for Kidney Transplantation focused on measuring Kidney Transplantation, Normothermic Machine Perfusion, Hypothermic Machine Perfusion
Eligibility Criteria
Inclusion Criteria: Age 18-60 years old, gender unlimited Volunteer to participate in this clinical trial and sign the informed consent form Suffering from end-stage renal disease Planned kidney transplantation The expanded standard donors (ECD) were obtained The expanded standard kidney donor is defined as: aged cadaver donors over 60 years old or two or three of the following conditions between 50 and 60 years old: death due to cerebrovascular disease; Hypertension; The blood creatinine level before donation was greater than 133umol/L. Exclusion Criteria: Active infection Living donor kidney recipient Acute rejection Second kidney transplantation Combined transplantation (combined liver and kidney, combined pancreas and kidney) Patients who take other clinical trial drugs or participate in other clinical studies Unable to continue the research Patients who are judged by the researcher to be unsuitable for this clinical trial
Sites / Locations
- China-Japan Friendship Hospital
Arms of the Study
Arm 1
Arm 2
Active Comparator
Experimental
Hypothermic machine perfusion
Normothermic machine perfusion
After organ acquisition, the donor kidney was trimmed, and then the donor kidney was connected to the cryoperfusion machine (lifeport, which has been used in routine clinical practice in our hospital) for continuous low temperature mechanical perfusion (<8 ℃). The perfusion solution was extracted for proteomic study at 10 min after perfusion and at the end of perfusion.
After conventional UW perfusion is obtained, the marginal donor kidney is immediately perfused without ischemia at normal temperature. The perfusion time is at least 4 hours (so as to repair the marginal donor kidney). The perfusion solution is as above, and the perfusion solution is loaded into the device authorized in Europe (XVIVO - KidneyAssist ®), Connect the transplanted renal artery with the device. Take 5ml perfusion solution in advance before perfusion, 5ml perfusion solution every 30min after perfusion, take perfusion solution at the end of perfusion (blood gas analysis for each perfusion solution, and finally perfusion solution for culture), and measure urine volume every hour. After perfusion, the transplanted kidney was disconnected from the perfusion device, and the donor kidney was perfused again with 2L HTK solution, followed by routine transplantation.