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Effects of Resistance Training Preconditioning on Skeletal Muscle Recovery From a Period of Disuse in Young Adults

Primary Purpose

Atrophy, Disuse, Atrophy, Muscular

Status
Not yet recruiting
Phase
Not Applicable
Locations
Study Type
Interventional
Intervention
Resistance Training Preconditioning
Control
Sponsored by
Auburn University
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional treatment trial for Atrophy, Disuse

Eligibility Criteria

18 Years - 30 Years (Adult)All SexesAccepts Healthy Volunteers

Inclusion Criteria: Between the ages of 18-30 Free from medical implants in lower extremity that would interfere with low-dose radiation Are not allergic to ultrasound gel or Lidocaine Have no or minimal experience with resistance training (less than 1 training session/week for a 1 year period) Exclusion Criteria: Have been exposed to medically necessary radiation in the past 12 months other than dental x-rays Have known overt cardiovascular or metabolic disease Have a medical condition that would contraindicate participating in an exercise program or donating a skeletal muscle biopsy (e.g. blood clotting disorder, taking blood thinners, etc.) Are undergoing medical treatment for a disease that would interfere physiologically or logistically with study outcomes and/or protocols (e.g. undergoing chemotherapy and/or radiation for cancer treatment) Have consumed supplemental protein, creatine, and/or agents that affect hormone status (e.g. testosterone, growth hormone boosters, etc.) within the previous 2 months.

Sites / Locations

    Arms of the Study

    Arm 1

    Arm 2

    Arm Type

    Experimental

    Active Comparator

    Arm Label

    Resistance Training Preconditioning (PRECON)

    Control (CTL)

    Arm Description

    This group will perform: 6 weeks of lower body focused resistance training 2 weeks of locking brace-induced immobilization of a randomized leg 6 weeks of lower body focused resistance training

    This group will perform: 6 weeks of activities of daily living (no training) 2 weeks of locking brace-induced immobilization of a randomized leg 6 weeks of lower body focused resistance training

    Outcomes

    Primary Outcome Measures

    Change in vastus lateralis fiber cross-sectional area
    Upon the procurement and analysis of muscle from the vastus lateralis of participants, histological techniques will be used to assess fiber cross-sectional area of muscle fibers
    Change in quadriceps strength via 3 repetition maximum testing
    Strength of the quadriceps will be assessed via 3-repetition maximum testing of select exercises (leg press, leg extension, hex-bar deadlift)
    Change in quadriceps strength via isokinetic dynamometry
    Strength of the quadriceps will be assessed via isokinetic dynamometry

    Secondary Outcome Measures

    Change in protein expression of anabolic signaling proteins
    Western blotting will be performed and quantified in relative fluorescence units for proteins involved in the mechanistic target of rapamycin signaling pathway.
    Change in protein expression of catabolic signaling proteins
    Western blotting will be performed and quantified in relative fluorescence units for proteins involved in the catabolic signaling pathways (e.g. ubiquitin-proteasome system, calpain system, autophagy).
    Change in activity of catabolic systems
    Activity assays will be performed and quantified in relative fluorescence units normalized to muscle soluble protein abundance in order to quantify the activity of the calpain system and the 20S proteasome core.
    Gene expression at the mRNA level of atrophy-associated genes (atrogenes)
    Real time quantitative polymerase chain reactions (qPCR) will be used to quantify the expression of select genes associated with atrophy. These data will be reported as fold-change from baseline and will be normalized to one or more housekeeping genes whose value does not change throughout the duration of the study.

    Full Information

    First Posted
    January 23, 2023
    Last Updated
    February 26, 2023
    Sponsor
    Auburn University
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    1. Study Identification

    Unique Protocol Identification Number
    NCT05760066
    Brief Title
    Effects of Resistance Training Preconditioning on Skeletal Muscle Recovery From a Period of Disuse in Young Adults
    Official Title
    Effects of Resistance Training Preconditioning on Skeletal Muscle Recovery From a Period of Disuse in Young Adults
    Study Type
    Interventional

    2. Study Status

    Record Verification Date
    February 2023
    Overall Recruitment Status
    Not yet recruiting
    Study Start Date
    August 1, 2023 (Anticipated)
    Primary Completion Date
    December 31, 2023 (Anticipated)
    Study Completion Date
    August 1, 2024 (Anticipated)

    3. Sponsor/Collaborators

    Responsible Party, by Official Title
    Principal Investigator
    Name of the Sponsor
    Auburn University

    4. Oversight

    Studies a U.S. FDA-regulated Drug Product
    No
    Studies a U.S. FDA-regulated Device Product
    No
    Product Manufactured in and Exported from the U.S.
    No
    Data Monitoring Committee
    No

    5. Study Description

    Brief Summary
    The goal of this clinical trial is to compare the effects of resistance training (RT) preconditioning vs no training on disuse-induced atrophy and post-disuse resistance training in young healthy individuals. The main questions it aims to answer are: To determine if performing RT prior to a period of disuse enhances the regain of strength, skeletal muscle size, and skeletal muscle quality while performing RT after a period of disuse. To determine if performing RT prior to a period of disuse dampens the maladaptive effects of disuse on muscle size, muscle quality, and strength. To determine the anabolic and proteolytic mechanisms underpinning the observed outcomes. Participants will: Perform either 6 weeks of resistance training or maintain an untrained lifestyle Perform 2 weeks of limb immobilization induced disuse of a randomized leg Perform 6 weeks of resistance training Researchers will compare the resistance training preconditioning condition vs the non-trained condition to see if resistance training prior to a period of disuse is beneficial during the disuse period and in the return to training period on skeletal muscle size, strength, and underpinning molecular markers.

    6. Conditions and Keywords

    Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
    Atrophy, Disuse, Atrophy, Muscular

    7. Study Design

    Primary Purpose
    Treatment
    Study Phase
    Not Applicable
    Interventional Study Model
    Parallel Assignment
    Masking
    None (Open Label)
    Allocation
    Randomized
    Enrollment
    24 (Anticipated)

    8. Arms, Groups, and Interventions

    Arm Title
    Resistance Training Preconditioning (PRECON)
    Arm Type
    Experimental
    Arm Description
    This group will perform: 6 weeks of lower body focused resistance training 2 weeks of locking brace-induced immobilization of a randomized leg 6 weeks of lower body focused resistance training
    Arm Title
    Control (CTL)
    Arm Type
    Active Comparator
    Arm Description
    This group will perform: 6 weeks of activities of daily living (no training) 2 weeks of locking brace-induced immobilization of a randomized leg 6 weeks of lower body focused resistance training
    Intervention Type
    Other
    Intervention Name(s)
    Resistance Training Preconditioning
    Intervention Description
    Resistance training prior to disuse-induced atrophy followed by another period of resistance training
    Intervention Type
    Other
    Intervention Name(s)
    Control
    Intervention Description
    Activities of daily living prior to disuse-induced atrophy followed by a period of resistance training
    Primary Outcome Measure Information:
    Title
    Change in vastus lateralis fiber cross-sectional area
    Description
    Upon the procurement and analysis of muscle from the vastus lateralis of participants, histological techniques will be used to assess fiber cross-sectional area of muscle fibers
    Time Frame
    Through completion of study, 16 weeks
    Title
    Change in quadriceps strength via 3 repetition maximum testing
    Description
    Strength of the quadriceps will be assessed via 3-repetition maximum testing of select exercises (leg press, leg extension, hex-bar deadlift)
    Time Frame
    Through completion of study, 16 weeks
    Title
    Change in quadriceps strength via isokinetic dynamometry
    Description
    Strength of the quadriceps will be assessed via isokinetic dynamometry
    Time Frame
    Through completion of study, 16 weeks
    Secondary Outcome Measure Information:
    Title
    Change in protein expression of anabolic signaling proteins
    Description
    Western blotting will be performed and quantified in relative fluorescence units for proteins involved in the mechanistic target of rapamycin signaling pathway.
    Time Frame
    Through completion of study, 16 weeks
    Title
    Change in protein expression of catabolic signaling proteins
    Description
    Western blotting will be performed and quantified in relative fluorescence units for proteins involved in the catabolic signaling pathways (e.g. ubiquitin-proteasome system, calpain system, autophagy).
    Time Frame
    Through completion of study, 16 weeks
    Title
    Change in activity of catabolic systems
    Description
    Activity assays will be performed and quantified in relative fluorescence units normalized to muscle soluble protein abundance in order to quantify the activity of the calpain system and the 20S proteasome core.
    Time Frame
    Through completion of study, 16 weeks
    Title
    Gene expression at the mRNA level of atrophy-associated genes (atrogenes)
    Description
    Real time quantitative polymerase chain reactions (qPCR) will be used to quantify the expression of select genes associated with atrophy. These data will be reported as fold-change from baseline and will be normalized to one or more housekeeping genes whose value does not change throughout the duration of the study.
    Time Frame
    Through completion of study, 16 weeks

    10. Eligibility

    Sex
    All
    Minimum Age & Unit of Time
    18 Years
    Maximum Age & Unit of Time
    30 Years
    Accepts Healthy Volunteers
    Accepts Healthy Volunteers
    Eligibility Criteria
    Inclusion Criteria: Between the ages of 18-30 Free from medical implants in lower extremity that would interfere with low-dose radiation Are not allergic to ultrasound gel or Lidocaine Have no or minimal experience with resistance training (less than 1 training session/week for a 1 year period) Exclusion Criteria: Have been exposed to medically necessary radiation in the past 12 months other than dental x-rays Have known overt cardiovascular or metabolic disease Have a medical condition that would contraindicate participating in an exercise program or donating a skeletal muscle biopsy (e.g. blood clotting disorder, taking blood thinners, etc.) Are undergoing medical treatment for a disease that would interfere physiologically or logistically with study outcomes and/or protocols (e.g. undergoing chemotherapy and/or radiation for cancer treatment) Have consumed supplemental protein, creatine, and/or agents that affect hormone status (e.g. testosterone, growth hormone boosters, etc.) within the previous 2 months.
    Central Contact Person:
    First Name & Middle Initial & Last Name or Official Title & Degree
    Michael D Roberts, PhD
    Phone
    334-844-1925
    Email
    mdr0024@auburn.edu
    First Name & Middle Initial & Last Name or Official Title & Degree
    John M Michel, MS
    Phone
    2059077830
    Email
    michel@auburn.edu

    12. IPD Sharing Statement

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    Effects of Resistance Training Preconditioning on Skeletal Muscle Recovery From a Period of Disuse in Young Adults

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