Tracing Changed Production of Red Blood Cells

In competitive sport, it is illegal to manipulate erythropoiesis. Manipulated erythropoiesis can indirectly be identified by atypical fluctuations in key haematological variables. However, this method also has limitations and as it is known that some athletes still manipulate erythropoiesis it is necessary to develop new and more sensitive detection methods. The primary purpose of the study is to examine the importance of altered erythropoiesis for surface and intracellular erythrocyte proteins, the number of immature reticulocytes, and for the haematological characteristics of the erythrocyte, such as volume, haemoglobin concentration and concentration of glycosylated haemoglobin, to assess whether these can be used to identify changed erythropoiesis. Furthermore, the aim is to examine whether these parameters are affected by freezer storage of erythrocytes.

Inclusion Criteria: Healthy men 18-40 years physical fitness rating more than 50ml/kg/min non-smokers blood pressure <130/90 mmHg hemoglobin concentration 7-10.5 mM. Exclusion Criteria: Blood donor who has donated blood within the last three months Participation in other concurrent clinical trials To participate in competitive sport during or three months after the study.