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Analysis of Circulating DNA in Blood Samples of Glioma-affected Patients

Primary Purpose

Glioma, Circulating Tumor Cell

Status
Recruiting
Phase
Not Applicable
Locations
Spain
Study Type
Interventional
Intervention
Liquid Biopsy
Sponsored by
Corporacion Parc Tauli
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional diagnostic trial for Glioma focused on measuring Liquid Biopsy, Glioma, Circulating free DNA, ddPCR

Eligibility Criteria

18 Years - undefined (Adult, Older Adult)All SexesDoes not accept healthy volunteers

Inclusion Criteria: Age ≥ 18 years. De novo primary brain lesion, compatible with a glioma according to contrast-enhanced magnetic resonance images. The injury must be treated by the usual surgical procedure. Signature of informed consent. Exclusion Criteria: Pregnant or breastfeeding women. Reasonable doubts in the diagnosis of glioma by magnetic resonance, or patients whose tissue biopsy diagnoses a brain lesion other than a glioma. Patients who cannot undergo surgical resection, or when the tissue samples taken are not significant enough to be analyzed. Inability or disagreement with signing the informed consent.

Sites / Locations

  • Hospital Universitari Parc TaulíRecruiting

Arms of the Study

Arm 1

Arm Type

Experimental

Arm Label

Circulating free DNA analysis

Arm Description

Correlate the results of the tissue samples with those of the blood samples, the concentration of ctDNA found, and the magnetic resonance images prior to the biopsies. Compare other variables such as time, money, and the impact on the patient (recovery time after biopsies, pain, sequelae,...) between the liquid biopsy procedure and the conventional tissue biopsy procedure.

Outcomes

Primary Outcome Measures

Circulating free DNA detection
Circulating free DNA detection in blood samples of patients by means of a ddPCR

Secondary Outcome Measures

Full Information

First Posted
April 4, 2023
Last Updated
August 29, 2023
Sponsor
Corporacion Parc Tauli
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1. Study Identification

Unique Protocol Identification Number
NCT05964153
Brief Title
Analysis of Circulating DNA in Blood Samples of Glioma-affected Patients
Official Title
Pilot Study of Liquid Biopsy as a Diagnostic Tool for Gliomas by Analyzing Circulating Tumor DNA in Blood Samples, and Its Validation With the Corresponding Tissue Biopsies.
Study Type
Interventional

2. Study Status

Record Verification Date
August 2023
Overall Recruitment Status
Recruiting
Study Start Date
January 31, 2023 (Actual)
Primary Completion Date
July 31, 2023 (Actual)
Study Completion Date
January 31, 2024 (Anticipated)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
Corporacion Parc Tauli

4. Oversight

Studies a U.S. FDA-regulated Drug Product
No
Studies a U.S. FDA-regulated Device Product
No
Data Monitoring Committee
Yes

5. Study Description

Brief Summary
The present pilot study aims to investigate a new strategy in the liquid biopsy protocol for the diagnosis of gliomas based on the detection of circulating tumor DNA in the blood of patients with brain lesions compatible with this type of tumor. In order to increase the sensitivity of the technique, the investigators will work with raw blood samples through minimally invasive procedures. The subsequent analysis will be done with digital PCR, due to its low detection limit. The mutational results of each patient's samples will be compared with those obtained from the corresponding tissue biopsies. This step will allow the team to determine the robustness and reliability of the liquid biopsy. The grading of the tumor, as well as the confirmation of the diagnosis, will be obtained from the histological data. With the inclusion of more patients in the future, and with the optimization of the mutations investigated, the investigators want to standardize the protocol for the diagnosis of gliomas with liquid biopsy. This technique is less invasive than current surgical procedures used for diagnosis. In addition, using fewer hospital resources should allow a more accurate and rapid diagnosis of the pathology, and therefore, start the more personalized therapeutic stage earlier.
Detailed Description
Currently, brain tumors are detected by means of an imaging technique (usually magnetic resonance imaging) with a contrast agent. The determination of the type of tumor, as well as its grading, is usually done subsequently with a biopsy of the tissue, where different solid samples are extracted as representative as possible to analyze them in the Pathological Anatomy service. Nevertheless, there are some factors that limit surgical access, such as: the advanced age of the patient, the location of the tumor in eloquent or risky areas, as well as the presence of large tumors with very diffuse borders. In these cases, the diagnosis of the brain tumor is usually made directly with the magnetic resonance image, which implies a risk of error due to the lack of clinical information from this test. Limitations like these make the minimally invasive procedure of liquid biopsy an extremely necessary diagnostic tool. For this reason, the investigators want to start a pilot study of this technique in those patients with brain lesions in the resonance images compatible with a glioma-like tumor. In particular, the main aim of the study is to analyze the blood samples obtained from these patients in order to detect and quantify the circulating DNA (ctDNA) of tumor cells on it. These DNA fragments are expelled into the bloodstream by mechanisms still unknown as a result of numerous processes of apoptosis and necrosis of tumor cells. By analyzing them through a ddPCR, the investigators will try to detect the specific mutations present in this tumoral ctDNA, allowing the team to confirm the presence of a glioma-type tumor, and providing real-time information of its classification as astrocytoma or oligodendroglioma.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Glioma, Circulating Tumor Cell
Keywords
Liquid Biopsy, Glioma, Circulating free DNA, ddPCR

7. Study Design

Primary Purpose
Diagnostic
Study Phase
Not Applicable
Interventional Study Model
Single Group Assignment
Masking
None (Open Label)
Allocation
N/A
Enrollment
10 (Anticipated)

8. Arms, Groups, and Interventions

Arm Title
Circulating free DNA analysis
Arm Type
Experimental
Arm Description
Correlate the results of the tissue samples with those of the blood samples, the concentration of ctDNA found, and the magnetic resonance images prior to the biopsies. Compare other variables such as time, money, and the impact on the patient (recovery time after biopsies, pain, sequelae,...) between the liquid biopsy procedure and the conventional tissue biopsy procedure.
Intervention Type
Diagnostic Test
Intervention Name(s)
Liquid Biopsy
Intervention Description
Analysis of the circulating free DNA in blood patients affected by gliomas
Primary Outcome Measure Information:
Title
Circulating free DNA detection
Description
Circulating free DNA detection in blood samples of patients by means of a ddPCR
Time Frame
1 year (2023-2024)

10. Eligibility

Sex
All
Minimum Age & Unit of Time
18 Years
Accepts Healthy Volunteers
No
Eligibility Criteria
Inclusion Criteria: Age ≥ 18 years. De novo primary brain lesion, compatible with a glioma according to contrast-enhanced magnetic resonance images. The injury must be treated by the usual surgical procedure. Signature of informed consent. Exclusion Criteria: Pregnant or breastfeeding women. Reasonable doubts in the diagnosis of glioma by magnetic resonance, or patients whose tissue biopsy diagnoses a brain lesion other than a glioma. Patients who cannot undergo surgical resection, or when the tissue samples taken are not significant enough to be analyzed. Inability or disagreement with signing the informed consent.
Central Contact Person:
First Name & Middle Initial & Last Name or Official Title & Degree
Coloma Moreno
Phone
+34937231010
Email
cmoreno@tauli.cat
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Silvia Gil
Organizational Affiliation
Hospital Universitari Parc Taulí
Official's Role
Principal Investigator
Facility Information:
Facility Name
Hospital Universitari Parc Taulí
City
Sabadell
State/Province
Barcelona
ZIP/Postal Code
08208
Country
Spain
Individual Site Status
Recruiting
Facility Contact:
First Name & Middle Initial & Last Name & Degree
Silvia Gil, PhD
Phone
+34937231010
Email
sgil@tauli.cat

12. IPD Sharing Statement

Plan to Share IPD
No

Learn more about this trial

Analysis of Circulating DNA in Blood Samples of Glioma-affected Patients

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