Microfluidic Chip Method Versus Density Gradient Centrifugation Method on Semen Parameters
Infertility
About this trial
This is an interventional treatment trial for Infertility focused on measuring Semen parameters, Microfluidic chip, Density Gradient Centrifugation
Eligibility Criteria
Inclusion Criteria: Sperm concentration of the raw semen with at least 5 million motile sperm per ml with a total volume of not less than 1.5ml. Exclusion Criteria: Sperm concentration of the raw semen of less than 5 million motile sperm per ml Men unable to provide an ejaculated semen sample
Sites / Locations
- Department of Obstetrics and GynaecologyRecruiting
Arms of the Study
Arm 1
Arm 2
Experimental
Active Comparator
Microfluidic Chip Method
Density Gradient Centrifugation Method
The Sperm Separation Device - ZyMōt Multi 850µL device (ZyMōt Fertility, Inc) will be used. The microfluidic chamber will be used based on the manufacturer's instructions. 850 μL of the semen sample will be added to the inlet port of the device and 750 μL of fertilization media will be added to the outlet port. The device will then be incubated in 6% CO2 at 37°C. After 30 minutes, 500 μL of the prepared sample at the outlet port will be removed and pipetted into a labelled test tube. The final volume will be adjusted to 1mL for sperm counting.
After liquefaction, sperm preparation will be completed by a discontinuous density gradient centrifugation method, using Pureception (CooperSurgical, Denmark) sperm density gradient media. The resulting sperm pellet after centrifugation will be washed once with the sperm washing medium (G-IVF Plus, Vitrolife, Sweden) The washed spermatozoa will be resuspended with the same medium, adjusting the final volume to 500 μL. The final volume will be adjusted to 1mL for sperm counting.