Microfluidic Chip vs Density Gradient Centrifugation on the Euploidy Rate of Pre-implantation Genetic Testing
Infertility, Genetic Disease, Chromosomal Rearrangement
About this trial
This is an interventional treatment trial for Infertility focused on measuring microfluidic chip, density gradient centrifugation, euploidy rate, pre-implantation genetic testing
Eligibility Criteria
Inclusion Criteria: Women aged <43 years at the time of ovarian stimulation for IVF Women undergoing PGT for monogenic diseases, structural rearrangement of chromosomes or aneuploidy Sperm concentration of the raw semen with at least 0.15 million motile sperm per ml or 100 motile sperm per 50 low power field (200x) of observation Exclusion Criteria: Use of frozen semen for insemination Use of donor oocytes and spermatozoa Submucosal fibroid or hydrosalpinx shown on pelvic scanning and not surgically treated; Women who had been recruited into this study before and Women joining other randomized trials
Sites / Locations
- Department of Obstetrics and Gynaecology
Arms of the Study
Arm 1
Arm 2
Experimental
Active Comparator
The microfluidic chip group
The density gradient centrifugation group
The Sperm Separation Device - ZyMōt Multi 850µL or 3 mL device (ZyMōt Fertility, Inc) will be used according to the volume of the raw semen samples. The microfluidics chamber will be used based on the manufacturer's instructions. 850 μL (850 μL device) or 3 mL (3mL device) of the semen sample will be added to the inlet port of the device and 750 μL (850 μL device) or 2.4 mL (3 mL device) of fertilization media will be added to the outlet port. The device will then be incubated in 6% CO2 at 37°C. After 30 minutes, 500 μL (850 μL device) or 1 mL (3mL device) of the prepared sample at the outlet port will be removed and pipetted into a labelled test tube.
After liquefaction, sperm preparation will be completed by a discontinuous density gradient centrifugation method, using Pureception (CooperSurgical, Denmark) sperm density gradient media. The resulting sperm pellet after centrifugation will be washed once with the sperm washing medium (G-IVF Plus, Vitrolife, Sweden) The washed spermatozoa will be resuspended with the same medium, adjusting the final volume to 0.5 mL.