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A Phase Ib Study of the Safety, Reactogenicity, and Immunogenicity of Sm-TSP-2/Alhydrogel)(R) With or Without AP 10-701 for Intestinal Schistosomiasis in Healthy Exposed Adults

Primary Purpose

Schistosomiasis

Status
Completed
Phase
Phase 1
Locations
Brazil
Study Type
Interventional
Intervention
GLA-AF
Hepatitis B Virus Vaccine (Recombinant)
Sm-TSP-2/Alhydrogel
Sponsored by
National Institute of Allergy and Infectious Diseases (NIAID)
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional prevention trial for Schistosomiasis focused on measuring AP 10-701, healthy adults, Immunogenicity, Phase Ib, Reactogenicity, Safety, Schistosomiasis, Sm-TSP-2/Alhydrogel®

Eligibility Criteria

18 Years - 50 Years (Adult)All SexesAccepts Healthy Volunteers

Inclusion Criteria:

  1. Provide written informed consent prior to any study procedures.
  2. Able to understand and comply with planned study procedures and be available for all study visits.
  3. Male or non-pregnant female aged 18 to 50, inclusive at the time of enrollment.
  4. Are in good health, as determined by vital signs (oral temperature, pulse, and blood pressure), medical history, and brief physical examination at screening.

    -Existing medical diagnoses or conditions (except those in the Subject Exclusion Criteria) must be deemed as stable chronic medical conditions. A stable chronic medical condition is defined as no change in prescription medication, dose, or frequency of medication in the last 3 months (90 days) and health outcomes of the specific disease are considered to be within acceptable limits in the last 6 months (180 days). Any change due to change of health care provider, or that is done for financial reasons, as long as in the same class of medication, will not be considered a violation of this inclusion criterion. Any change in prescription medication due to improvement of a disease outcome, as determined by the site principal investigator or appropriate sub-investigator, will not be considered a violation of this inclusion criterion. Subjects may be on chronic or as needed medications if, in the opinion of the site principal investigator or appropriate sub-investigator, they pose no additional risk to subject safety or assessment of reactogenicity and immunogenicity. Topical, nasal, and inhaled medications (with the exception of corticosteroids as outlined in the Subjects Exclusion Criteria), vitamins, and contraceptives are permitted.

  5. Vital signs (oral temperature, pulse, and blood pressure) are all within normal protocol-defined ranges.

    -The normal protocol-defined ranges for vital signs include (a) oral temperature less than 38.0 degrees celsius, (b) pulse 50 to 100 bpm, inclusive, (c) systolic blood pressure 85 to 150 mmHg, inclusive, and (d) diastolic blood pressure 55 to 90 mmHg, inclusive. Pulse rate <50 is acceptable for 2nd and 3rd vaccinations if the subject is otherwise healthy with documented sinus bradycardia at baseline.

  6. Laboratory tests (alanine aminotransferase, creatinine, white blood cell count, hemoglobin, and platelets) are all within protocol-defined reference ranges.

    -The protocol-defined ranges for laboratory tests include (a) alanine aminotransferase (ALT) of less than 1.25-times the upper reference limit, (b) creatinine less than 1.25 times the upper reference limit (c) white blood cells (WBC) between 3.3 x10^3/uL and 10.4 x10^3/uL, inclusive, (d) hemoglobin 11.4 g/dL or greater for females or 12.1 g/dL or greater for males, (e) platelets greater than 130 x10^3/uL. Laboratory test results for 2nd and 3rd vaccinations may be at Grade 1 if considered unrelated to study product.

  7. Urinalysis with no greater than trace protein and negative for glucose.
  8. Female subjects of childbearing potential must agree to practice highly effective contraception for a minimum of 30 days prior to study product exposure and for 30 days after last vaccination.

    • Female subjects who are surgically sterile via tubal sterilization, bilateral oophorectomy or hysterectomy or who have been postmenopausal for greater than 1 year are not considered to be of childbearing potential.
    • Highly effective methods of contraception are defined as having low failure rates (i.e. less than 1% per year) when used consistently and correctly and may include, but are not limited to, abstinence from intercourse with a male partner, monogamous relationship with a vasectomized partner, male condoms or diaphragm with spermicide, intrauterine devices, and licensed hormonal methods.
  9. Female subjects of childbearing potential must have a negative urine or serum pregnancy test within 24 hours prior to study vaccination.
  10. Able to correctly answer all questions on the informed consent comprehension questionnaire.

Exclusion Criteria:

  1. Has the intention to become pregnant within 5 months after enrollment in this study.
  2. Female subjects who are breastfeeding or plan to breastfeed at any given time from the first study vaccination until 30 days after their last study vaccination.
  3. Has an acute illness, including a documented oral temperature of 38.0 degrees celsius or greater, within 72 hours prior to vaccination.
  4. Evidence of clinically significant neurologic, cardiac, pulmonary, hepatic, rheumatologic, autoimmune, diabetes, or renal disease by history, physical examination, and/or laboratory studies.
  5. Is immunosuppressed as a result of an underlying illness or treatment.

    -Causes for immunosuppression may include, but are not limited to, poorly-controlled diabetes mellitus, cirrhosis, renal insufficiency, active neoplastic disease or a history of any hematologic malignancy, connective tissue disease, organ transplant.

  6. Using or intends to continue using oral or parenteral steroids, high-dose inhaled steroids (>800 µg/day of beclomethasone dipropionate or equivalent) or other immunosuppressive or cytotoxic drugs.
  7. Positive hepatitis B surface antigen (HBsAg)
  8. Positive confirmatory test for HIV infection
  9. Positive confirmatory test for hepatitis C virus (HCV) infection
  10. Volunteer has had a history of alcohol or illicit drug abuse during the past 24 months.
  11. Received immunoglobulin or other blood products (with exception of Rho D immunoglobulin) within 90 days prior to study vaccination.
  12. History of a severe allergic reaction or anaphylaxis to known components of the study vaccines.
  13. Has an acute or chronic medical condition that, in the opinion of the investigator, would render participation in this study unsafe or would interfere with the evaluation of responses.

    -This includes, but is not limited to: known liver disease, renal disease, neurological disorders, visual field defects, cardiac disorders, pulmonary disorders, diabetes mellitus, and transplant recipients.

  14. History of splenectomy
  15. Is participating or plans to participate in another clinical trial with an interventional agent during the duration of the study.

    -This may include other licensed or unlicensed vaccines, drugs, biologics, devices, blood products, or medications.

  16. Received any licensed live vaccine within 30 days or any licensed inactivated vaccine within 14 days prior to the first study vaccination.
  17. Planned receipt of any vaccine from the first study vaccination through 28 days after the last study vaccination.
  18. Has any diagnosis, current or past, of schizophrenia, bipolar disease, or other psychiatric diagnosis that may interfere with subject compliance or safety evaluations.
  19. Has any condition that would, in the opinion of the site investigator, place the subject at an unacceptable risk of injury or render the subject unable to meet the requirements of the protocol.
  20. Anti-Sm-TSP-2 IgE antibody level above ELISA reactivity threshold.

Sites / Locations

  • Americaninhas Vaccine Center

Arms of the Study

Arm 1

Arm 2

Arm 3

Arm 4

Arm 5

Arm 6

Arm 7

Arm Type

Experimental

Experimental

Experimental

Experimental

Experimental

Experimental

Active Comparator

Arm Label

Group A

Group B

Group D

Group E

Group G

Group H

Pooled Active Comparator Group

Arm Description

10mcg Sm-TSP-2/Alhydrogel® (n=8)

10mcg Sm-TSP-2/Alhydrogel®/+ AP 10-701 (n=8)

30mcg Sm-TSP-2/Alhydrogel® (n=8)

30mcg Sm-TSP-2/Alhydrogel®/+ AP 10-701 (n=8)

100mcg Sm-TSP-2/Alhydrogel® (n=8)

100mcg Sm-TSP-2/Alhydrogel®/+ AP 10-701 (n=8)

Euvax B Hepatitis B vaccine (n=12)

Outcomes

Primary Outcome Measures

The occurrence of new-onset chronic medical conditions (including AESI)
The occurrence of solicited injection site reactogenicity
The occurrence of solicited injection site reactogenicity
The occurrence of solicited injection site reactogenicity
The occurrence of solicited systemic reactogenicity
The occurrence of solicited systemic reactogenicity
The occurrence of solicited systemic reactogenicity
The occurrence of study vaccine-related SAEs
The occurrence of vaccine-related clinical safety laboratory adverse events
The occurrence of vaccine-related clinical safety laboratory adverse events
The occurrence of vaccine-related clinical safety laboratory adverse events
The occurrence of vaccine-related clinical safety laboratory adverse events
The occurrence of vaccine-related clinical safety laboratory adverse events

Secondary Outcome Measures

The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
The anti-Sm-TSP-2 IgG level using an indirect ELISA

Full Information

First Posted
March 21, 2017
Last Updated
January 28, 2021
Sponsor
National Institute of Allergy and Infectious Diseases (NIAID)
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1. Study Identification

Unique Protocol Identification Number
NCT03110757
Brief Title
A Phase Ib Study of the Safety, Reactogenicity, and Immunogenicity of Sm-TSP-2/Alhydrogel)(R) With or Without AP 10-701 for Intestinal Schistosomiasis in Healthy Exposed Adults
Official Title
A Phase Ib Study of the Safety, Reactogenicity, and Immunogenicity of Sm-TSP-2/Alhydrogel(R) With or Without AP 10-701 for Intestinal Schistosomiasis in Healthy Exposed Adults
Study Type
Interventional

2. Study Status

Record Verification Date
January 26, 2021
Overall Recruitment Status
Completed
Study Start Date
May 20, 2018 (Actual)
Primary Completion Date
November 14, 2019 (Actual)
Study Completion Date
November 14, 2019 (Actual)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Sponsor
Name of the Sponsor
National Institute of Allergy and Infectious Diseases (NIAID)

4. Oversight

Studies a U.S. FDA-regulated Drug Product
Yes
Studies a U.S. FDA-regulated Device Product
No
Product Manufactured in and Exported from the U.S.
No

5. Study Description

Brief Summary
The study will be conducted as a randomized, controlled, double blind Phase 1b dose-escalating clinical trial in up to 60 healthy adult males and non-pregnant females living in the S. mansoni-endemic area of Americaninhas, Brazil. The primary objective of this trial is to assess the safety and reactogenicity of ascending doses of Sm-TSP-2/Alhydrogel(R) (10mcg, 30mcg, or 100mcg) vaccine with or without AP 10-701 given as three doses administered on Days 1, 57, and 113.
Detailed Description
The study will be conducted as a randomized, controlled, double blind Phase 1b dose-escalating clinical trial in up to 60 healthy adult males and non-pregnant females living in the S. mansoni-endemic area of Americaninhas, Brazil. The study will recruit up to 60 healthy adult males and non-pregnant females to test two formulations of Sm-TSP-2 vaccine (adjuvanted with Alhydrogel(R) only, or with Alhydrogel(R) plus AP 10-701), each at 3 different doses of antigen: 10mcg, 30mcg, and 100mcg. The study will use a dose-escalation cohort design, in which escalation to the next dose cohort will be determined based on evaluation of pre-defined escalation criteria requiring 7 day safety data to be examined after all subjects in the current cohort have received their first dose of vaccine. Cohorts will be enrolled sequentially. For each Cohort (1-3), an initial 5 subjects (2 Sm-TSP-2/Alhydrogel(R), 2 Sm-TSP-2/Alhydrogel(R)/AP 10-701, and 1 Euvax B Hepatitis B vaccine) will be enrolled, randomized, vaccinated, and have completed Visit 02 (Day 2), before enrolling the rest of the cohort. As with dose-escalation decisions, evidence of significant reactogenicity will require further review prior to proceeding. The primary objective of this trial is to assess the safety and reactogenicity of ascending doses of Sm-TSP-2/Alhydrogel(R) (10mcg, 30mcg, or 100mcg) vaccine with or without AP 10-701 given as three doses administered on Days 1, 57, and 113. The secondary objectives used to evaluate the immunogenicity are: (1) to assess the IgG antibody response to Sm-TSP-2 using an indirect enzyme-linked immunosorbent assay (ELISA) at Day 127, (2) to assess the IgG antibody response to Sm-TSP-2 using an indirect ELISA at 14 days after dose one and two and Days 203, 293, and 478 (3, 6, and 12 months after the third dose) of Sm-TSP-2/Alhydrogel(R) (10mcg, 30mcg, or 100mcg) with or without AP 10-701, and (3) to assess the duration of the IgG antibody response to Sm-TSP-2 using an indirect ELISA following receipt of three doses of Sm-TSP-2/Alhydrogel(R) (10mcg, 30mcg, or 100mcg) with or without AP 10-701.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Schistosomiasis
Keywords
AP 10-701, healthy adults, Immunogenicity, Phase Ib, Reactogenicity, Safety, Schistosomiasis, Sm-TSP-2/Alhydrogel®

7. Study Design

Primary Purpose
Prevention
Study Phase
Phase 1
Interventional Study Model
Sequential Assignment
Masking
ParticipantInvestigator
Allocation
Randomized
Enrollment
60 (Actual)

8. Arms, Groups, and Interventions

Arm Title
Group A
Arm Type
Experimental
Arm Description
10mcg Sm-TSP-2/Alhydrogel® (n=8)
Arm Title
Group B
Arm Type
Experimental
Arm Description
10mcg Sm-TSP-2/Alhydrogel®/+ AP 10-701 (n=8)
Arm Title
Group D
Arm Type
Experimental
Arm Description
30mcg Sm-TSP-2/Alhydrogel® (n=8)
Arm Title
Group E
Arm Type
Experimental
Arm Description
30mcg Sm-TSP-2/Alhydrogel®/+ AP 10-701 (n=8)
Arm Title
Group G
Arm Type
Experimental
Arm Description
100mcg Sm-TSP-2/Alhydrogel® (n=8)
Arm Title
Group H
Arm Type
Experimental
Arm Description
100mcg Sm-TSP-2/Alhydrogel®/+ AP 10-701 (n=8)
Arm Title
Pooled Active Comparator Group
Arm Type
Active Comparator
Arm Description
Euvax B Hepatitis B vaccine (n=12)
Intervention Type
Biological
Intervention Name(s)
GLA-AF
Intervention Description
Previously referred to as Gluco-pyranosylphospho-lipid A aqueous formulation (GLA-AF). It is a toll-like receptor-4 agonist
Intervention Type
Biological
Intervention Name(s)
Hepatitis B Virus Vaccine (Recombinant)
Intervention Description
A non-infectious subunit viral vaccine derived from hepatitis B surface antigen (HBsAg) produced in yeast cells.
Intervention Type
Biological
Intervention Name(s)
Sm-TSP-2/Alhydrogel
Intervention Description
Sm-TSP-2/Alhydrogel
Primary Outcome Measure Information:
Title
The occurrence of new-onset chronic medical conditions (including AESI)
Time Frame
From Day 1 to Day 478
Title
The occurrence of solicited injection site reactogenicity
Time Frame
From Day 1 to Day 7
Title
The occurrence of solicited injection site reactogenicity
Time Frame
From Day 113 to Day 120
Title
The occurrence of solicited injection site reactogenicity
Time Frame
From Day 57 to Day 64
Title
The occurrence of solicited systemic reactogenicity
Time Frame
From Day 1 to Day 7
Title
The occurrence of solicited systemic reactogenicity
Time Frame
From Day 113 to Day 120
Title
The occurrence of solicited systemic reactogenicity
Time Frame
From Day 57 to Day 64
Title
The occurrence of study vaccine-related SAEs
Time Frame
From Day 1 to Day 478
Title
The occurrence of vaccine-related clinical safety laboratory adverse events
Time Frame
Day 113
Title
The occurrence of vaccine-related clinical safety laboratory adverse events
Time Frame
Day 120
Title
The occurrence of vaccine-related clinical safety laboratory adverse events
Time Frame
Day 57
Title
The occurrence of vaccine-related clinical safety laboratory adverse events
Time Frame
Day 64
Title
The occurrence of vaccine-related clinical safety laboratory adverse events
Time Frame
Day 8
Secondary Outcome Measure Information:
Title
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
Time Frame
Day 15
Title
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
Time Frame
Day 203
Title
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
Time Frame
Day 293
Title
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
Time Frame
Day 478
Title
The anti-Sm-TSP-2 IgG antibody response using an indirect ELISA
Time Frame
Day 71
Title
The anti-Sm-TSP-2 IgG level using an indirect ELISA
Time Frame
Day 127

10. Eligibility

Sex
All
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
50 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria: Provide written informed consent prior to any study procedures. Able to understand and comply with planned study procedures and be available for all study visits. Male or non-pregnant female aged 18 to 50, inclusive at the time of enrollment. Are in good health, as determined by vital signs (oral temperature, pulse, and blood pressure), medical history, and brief physical examination at screening. -Existing medical diagnoses or conditions (except those in the Subject Exclusion Criteria) must be deemed as stable chronic medical conditions. A stable chronic medical condition is defined as no change in prescription medication, dose, or frequency of medication in the last 3 months (90 days) and health outcomes of the specific disease are considered to be within acceptable limits in the last 6 months (180 days). Any change due to change of health care provider, or that is done for financial reasons, as long as in the same class of medication, will not be considered a violation of this inclusion criterion. Any change in prescription medication due to improvement of a disease outcome, as determined by the site principal investigator or appropriate sub-investigator, will not be considered a violation of this inclusion criterion. Subjects may be on chronic or as needed medications if, in the opinion of the site principal investigator or appropriate sub-investigator, they pose no additional risk to subject safety or assessment of reactogenicity and immunogenicity. Topical, nasal, and inhaled medications (with the exception of corticosteroids as outlined in the Subjects Exclusion Criteria), vitamins, and contraceptives are permitted. Vital signs (oral temperature, pulse, and blood pressure) are all within normal protocol-defined ranges. -The normal protocol-defined ranges for vital signs include (a) oral temperature less than 38.0 degrees celsius, (b) pulse 50 to 100 bpm, inclusive, (c) systolic blood pressure 85 to 150 mmHg, inclusive, and (d) diastolic blood pressure 55 to 90 mmHg, inclusive. Pulse rate <50 is acceptable for 2nd and 3rd vaccinations if the subject is otherwise healthy with documented sinus bradycardia at baseline. Laboratory tests (alanine aminotransferase, creatinine, white blood cell count, hemoglobin, and platelets) are all within protocol-defined reference ranges. -The protocol-defined ranges for laboratory tests include (a) alanine aminotransferase (ALT) of less than 1.25-times the upper reference limit, (b) creatinine less than 1.25 times the upper reference limit (c) white blood cells (WBC) between 3.3 x10^3/uL and 10.4 x10^3/uL, inclusive, (d) hemoglobin 11.4 g/dL or greater for females or 12.1 g/dL or greater for males, (e) platelets greater than 130 x10^3/uL. Laboratory test results for 2nd and 3rd vaccinations may be at Grade 1 if considered unrelated to study product. Urinalysis with no greater than trace protein and negative for glucose. Female subjects of childbearing potential must agree to practice highly effective contraception for a minimum of 30 days prior to study product exposure and for 30 days after last vaccination. Female subjects who are surgically sterile via tubal sterilization, bilateral oophorectomy or hysterectomy or who have been postmenopausal for greater than 1 year are not considered to be of childbearing potential. Highly effective methods of contraception are defined as having low failure rates (i.e. less than 1% per year) when used consistently and correctly and may include, but are not limited to, abstinence from intercourse with a male partner, monogamous relationship with a vasectomized partner, male condoms or diaphragm with spermicide, intrauterine devices, and licensed hormonal methods. Female subjects of childbearing potential must have a negative urine or serum pregnancy test within 24 hours prior to study vaccination. Able to correctly answer all questions on the informed consent comprehension questionnaire. Exclusion Criteria: Has the intention to become pregnant within 5 months after enrollment in this study. Female subjects who are breastfeeding or plan to breastfeed at any given time from the first study vaccination until 30 days after their last study vaccination. Has an acute illness, including a documented oral temperature of 38.0 degrees celsius or greater, within 72 hours prior to vaccination. Evidence of clinically significant neurologic, cardiac, pulmonary, hepatic, rheumatologic, autoimmune, diabetes, or renal disease by history, physical examination, and/or laboratory studies. Is immunosuppressed as a result of an underlying illness or treatment. -Causes for immunosuppression may include, but are not limited to, poorly-controlled diabetes mellitus, cirrhosis, renal insufficiency, active neoplastic disease or a history of any hematologic malignancy, connective tissue disease, organ transplant. Using or intends to continue using oral or parenteral steroids, high-dose inhaled steroids (>800 µg/day of beclomethasone dipropionate or equivalent) or other immunosuppressive or cytotoxic drugs. Positive hepatitis B surface antigen (HBsAg) Positive confirmatory test for HIV infection Positive confirmatory test for hepatitis C virus (HCV) infection Volunteer has had a history of alcohol or illicit drug abuse during the past 24 months. Received immunoglobulin or other blood products (with exception of Rho D immunoglobulin) within 90 days prior to study vaccination. History of a severe allergic reaction or anaphylaxis to known components of the study vaccines. Has an acute or chronic medical condition that, in the opinion of the investigator, would render participation in this study unsafe or would interfere with the evaluation of responses. -This includes, but is not limited to: known liver disease, renal disease, neurological disorders, visual field defects, cardiac disorders, pulmonary disorders, diabetes mellitus, and transplant recipients. History of splenectomy Is participating or plans to participate in another clinical trial with an interventional agent during the duration of the study. -This may include other licensed or unlicensed vaccines, drugs, biologics, devices, blood products, or medications. Received any licensed live vaccine within 30 days or any licensed inactivated vaccine within 14 days prior to the first study vaccination. Planned receipt of any vaccine from the first study vaccination through 28 days after the last study vaccination. Has any diagnosis, current or past, of schizophrenia, bipolar disease, or other psychiatric diagnosis that may interfere with subject compliance or safety evaluations. Has any condition that would, in the opinion of the site investigator, place the subject at an unacceptable risk of injury or render the subject unable to meet the requirements of the protocol. Anti-Sm-TSP-2 IgE antibody level above ELISA reactivity threshold.
Facility Information:
Facility Name
Americaninhas Vaccine Center
City
Novo Oriente
State/Province
Minas Gerais
Country
Brazil

12. IPD Sharing Statement

Learn more about this trial

A Phase Ib Study of the Safety, Reactogenicity, and Immunogenicity of Sm-TSP-2/Alhydrogel)(R) With or Without AP 10-701 for Intestinal Schistosomiasis in Healthy Exposed Adults

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