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Administration of Donor T Cells With the Caspase-9 Suicide Gene (DOTTI)

Primary Purpose

Acute Lymphoblastic Leukemia, Myelodysplastic Syndrome, Acute Myeloid Leukemia

Status
Active
Phase
Phase 1
Locations
United States
Study Type
Interventional
Intervention
iCaspase9-transduced T cells
AP1903
Sponsored by
Baylor College of Medicine
About
Eligibility
Locations
Arms
Outcomes
Full info

About this trial

This is an interventional treatment trial for Acute Lymphoblastic Leukemia focused on measuring Acute lymphoblastic leukemia, Myelodysplastic syndrome, Acute myeloid leukemia, Chronic myelogenous leukemia, Non Hodgkin lymphoma, Hemophagocytic lymphohistiocytosis, Familial hemophagocytic lymphohistiocytosis, Hemophagocytic syndrome, Epstein Barr virus infection, X-linked lymphoproliferative disease

Eligibility Criteria

undefined - undefined (Child, Adult, Older Adult)All SexesDoes not accept healthy volunteers

Inclusion Criteria:

Inclusion Criteria at Time of Procurement:

  1. Lack of a suitable conventional donor (i.e. 5/6 or 6/6 related, or 5/6 or 6/6 unrelated donor), or presence of a rapidly progressive disease not permitting time to identify an unrelated donor.
  2. High risk disease in one of the following:

    • Myelodysplastic syndrome (MDS) in one of the following categories: RCMD with an IPSS-R of intermediate, poor, or very poor, RAEB-1, or RAEB-2
    • Acute myeloid leukemia (AML) after first relapse or primary refractory disease
    • Chronic myelogenous leukemia (CML) in Chronic Phase 2 or greater, Accelerated Phase or Blast Crisis
    • Acute lymphoblastic leukemia (ALL) after first relapse or primary refractory disease, or High-Grade Non Hodgkin lymphoma (NHL) Stage III or IV after first relapse or primary refractory disease
    • Hemophagocytic lymphohistiocytosis (HLH)
    • Familial hemophagocytic lymphohistiocytosis (FLH)
    • Viral-associated hemophagocytic syndrome (VAHS)
    • T or NK cell lymphoproliferative syndrome
    • X-linked lymphoproliferative disease (XLP)

Inclusion Criteria at Time of T Cell Infusion:

  1. Engrafted with an absolute neutrophil count (ANC) > 500 cells/µL
  2. Greater than or equal to 50% donor chimerism in either peripheral blood or bone marrow, or relapse of their original disease
  3. Life expectancy > 30 days
  4. Lansky/Karnofsky score greater than or equal to 60
  5. Absence of severe renal disease (creatinine > 2X upper limit of normal for age)
  6. Absence of severe hepatic disease (direct bilirubin > 3X upper limit of normal or SGOT > 3X upper limit of normal)
  7. Oxygen saturation > 94% on room air
  8. Patient/Guardian able to give informed consent
  9. AP1903 available in sufficient quantities to allow for treatment of the patient

Exclusion Criteria:

Exclusion Criteria at Time of T Cell Infusion:

  1. GvHD
  2. Severe intercurrent infection
  3. Pregnancy*
  4. Other investigational drugs in the prior 30 days

    • Pregnancy test only required for at-risk individuals, defined as female patients of childbearing potential who have received a reduced-intensity conditioning regimen.

Sites / Locations

  • Texas Children's Hospital
  • The Methodist Hospital

Arms of the Study

Arm 1

Arm Type

Experimental

Arm Label

iCaspase9-transduced T cells

Arm Description

The 5 dose levels are: 1 x 10^4 T cells/kg 1 x 10^5 T cells/kg 5 x 10^5 T cells/kg 1 x 10^6 T cells/kg 5 x 10^6 T cells/kg AP1903 will be administered if there is development of Grade 1 or greater GvHD.

Outcomes

Primary Outcome Measures

Clinical and immunological effects of AP1903 administration.
To evaluate the clinical and immunological effects of AP1903 administration, a dimerizer drug used to activate an iCaspase9 suicide gene mechanism, to subjects who have received escalating doses of T lymphocytes expressing the iCaspase9 gene and developed acute graft-versus-host-disease (GvHD).

Secondary Outcome Measures

T cell dose that produces a greater than 25% risk of Grade II or greater GvHD.
To discover the number of T cells/kg (up to 5 x 10^6/kg) that produce a greater than 25% risk of inducing Grade II or greater acute GvHD in these subjects.
Immune reconstitution and relative contribution of iCaspase9-modified T cells post-infusion.
To measure the subsequent immune reconstitution of recipients of iCaspase9 modified T cells (and dimerizer drug), and assess the relative contribution of endogenous T cell recovery and infused gene-modified T cells.
Overall and disease-free survival.
To measure the overall and disease-free survival of recipients of iCaspase9 T cells at 100 days and at 1 year post-transplant.

Full Information

First Posted
December 15, 2011
Last Updated
July 6, 2023
Sponsor
Baylor College of Medicine
Collaborators
The Methodist Hospital Research Institute, Center for Cell and Gene Therapy, Baylor College of Medicine
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1. Study Identification

Unique Protocol Identification Number
NCT01494103
Brief Title
Administration of Donor T Cells With the Caspase-9 Suicide Gene
Acronym
DOTTI
Official Title
Administration of Haploidentical Donor T Cells Transduced With the Inducible Caspase-9 Suicide Gene
Study Type
Interventional

2. Study Status

Record Verification Date
July 2023
Overall Recruitment Status
Active, not recruiting
Study Start Date
November 2011 (undefined)
Primary Completion Date
May 2029 (Anticipated)
Study Completion Date
May 2029 (Anticipated)

3. Sponsor/Collaborators

Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
Baylor College of Medicine
Collaborators
The Methodist Hospital Research Institute, Center for Cell and Gene Therapy, Baylor College of Medicine

4. Oversight

Studies a U.S. FDA-regulated Drug Product
Yes
Studies a U.S. FDA-regulated Device Product
No
Data Monitoring Committee
Yes

5. Study Description

Brief Summary
Patients will be receiving a stem cell transplant as treatment for their disease. As part of the stem cell transplant, patients will be given very strong doses of chemotherapy, which will kill all their existing stem cells. A close relative of the patient will be identified, whose stem cells are not a perfect match for the patient's, but can be used. This type of transplant is called "allogeneic", meaning that the cells are from a donor. With this type of donor who is not a perfect match, there is typically an increased risk of developing GvHD, and a longer delay in the recovery of the immune system. GvHD is a serious and sometimes fatal side-effect of stem cell transplant. GvHD occurs when the new donor cells (graft) recognize that the body tissues of the patient (host) are different from those of the donor. In this study, investigators are trying to see whether they can make special T cells in the laboratory that can be given to the patient to help their immune system recover faster. As a safety measure, we want to "program" the T cells so that if, after they have been given to the patient, they start to cause GvHD, we can destroy them ("suicide gene"). Investigators will obtain T cells from a donor, culture them in the laboratory, and then introduce the "suicide gene" which makes the cells sensitive to a specific drug called AP1903. If the specially modified T cells begin to cause GvHD, the investigators can kill the cells by administering AP1903 to the patient. We have had encouraging results in a previous study regarding the effective elimination of T cells causing GvHD, while sparing a sufficient number of T cells to fight infection and potentially cancer. More specifically, T cells made to carry a gene called iCasp9 can be killed when they encounter the drug AP1903. To get the iCasp9 gene into T cells, we insert it using a virus called a retrovirus that has been made for this study. The AP1903 that will be used to "activate" the iCasp9 is an experimental drug that has been tested in a study in normal donors with no bad side-effects. We hope we can use this drug to kill the T cells. The major purpose of this study is to find a safe and effective dose of "iCasp9" T cells that can be given to patients who receive an allogeneic stem cell transplant. Another important purpose of this study is to find out whether these special T cells can help the patient's immune system recover faster after the transplant than they would have otherwise.
Detailed Description
If the patient is doing well after the stem cell transplant, and does not have severe GvHD, s/he will be eligible to receive the special "iCasp9" T cells from Day 30 to 90 after transplant. The specially selected and treated T cells will be given by vein (IV) once. This is a dose escalation study. This means that at the beginning, patients will be started on the lowest dose (1 of 5 different levels) of T cells. Once that dose schedule proves safe, the next group of patients will be started at a higher dose. This process will continue until all 5 dose levels are studied. If the side-effects are too severe, the dose will be lowered or the T cell injections will be stopped. If the patient develops GvHD after being given the specially treated T cells, we will prescribe AP1903, which has been shown to kill cells carrying the iCasp9 gene. This drug will be given as a 2-hour IV infusion. We will continue to follow the patient weekly in the bone marrow transplant clinic for the first month after the infusion, to check for side-effects of the treatment and for GvHD. The patient will have the standard tests performed that all patients have after transplant, even when not receiving special T cells.

6. Conditions and Keywords

Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Acute Lymphoblastic Leukemia, Myelodysplastic Syndrome, Acute Myeloid Leukemia, Chronic Myelogenous Leukemia, Non Hodgkin Lymphoma, Hemophagocytic Lymphohistiocytosis, Familial Hemophagocytic Lymphohistiocytosis, Hemophagocytic Syndrome, Epstein Barr Virus Infection, X-linked Lymphoproliferative Disease
Keywords
Acute lymphoblastic leukemia, Myelodysplastic syndrome, Acute myeloid leukemia, Chronic myelogenous leukemia, Non Hodgkin lymphoma, Hemophagocytic lymphohistiocytosis, Familial hemophagocytic lymphohistiocytosis, Hemophagocytic syndrome, Epstein Barr virus infection, X-linked lymphoproliferative disease

7. Study Design

Primary Purpose
Treatment
Study Phase
Phase 1
Interventional Study Model
Single Group Assignment
Masking
None (Open Label)
Allocation
N/A
Enrollment
15 (Actual)

8. Arms, Groups, and Interventions

Arm Title
iCaspase9-transduced T cells
Arm Type
Experimental
Arm Description
The 5 dose levels are: 1 x 10^4 T cells/kg 1 x 10^5 T cells/kg 5 x 10^5 T cells/kg 1 x 10^6 T cells/kg 5 x 10^6 T cells/kg AP1903 will be administered if there is development of Grade 1 or greater GvHD.
Intervention Type
Biological
Intervention Name(s)
iCaspase9-transduced T cells
Intervention Description
Patients will receive the T cells between 30 and 90 days following transplantation. The T cells will be infused through a catheter line.
Intervention Type
Drug
Intervention Name(s)
AP1903
Other Intervention Name(s)
AP1903 Dimerizer Drug
Intervention Description
AP1903 will be administered if there is development of Grade 1 or greater GvHD. Dose: 0.4 mg/kg by IV over 2 hours. Up to 3 additional doses may be administered if the GvHD does not respond or gets worse.
Primary Outcome Measure Information:
Title
Clinical and immunological effects of AP1903 administration.
Description
To evaluate the clinical and immunological effects of AP1903 administration, a dimerizer drug used to activate an iCaspase9 suicide gene mechanism, to subjects who have received escalating doses of T lymphocytes expressing the iCaspase9 gene and developed acute graft-versus-host-disease (GvHD).
Time Frame
14 days
Secondary Outcome Measure Information:
Title
T cell dose that produces a greater than 25% risk of Grade II or greater GvHD.
Description
To discover the number of T cells/kg (up to 5 x 10^6/kg) that produce a greater than 25% risk of inducing Grade II or greater acute GvHD in these subjects.
Time Frame
42 days
Title
Immune reconstitution and relative contribution of iCaspase9-modified T cells post-infusion.
Description
To measure the subsequent immune reconstitution of recipients of iCaspase9 modified T cells (and dimerizer drug), and assess the relative contribution of endogenous T cell recovery and infused gene-modified T cells.
Time Frame
Up to 15 years
Title
Overall and disease-free survival.
Description
To measure the overall and disease-free survival of recipients of iCaspase9 T cells at 100 days and at 1 year post-transplant.
Time Frame
100 days and 1 year

10. Eligibility

Sex
All
Accepts Healthy Volunteers
No
Eligibility Criteria
Inclusion Criteria: Inclusion Criteria at Time of Procurement: Lack of a suitable conventional donor (i.e. 5/6 or 6/6 related, or 5/6 or 6/6 unrelated donor), or presence of a rapidly progressive disease not permitting time to identify an unrelated donor. High risk disease in one of the following: Myelodysplastic syndrome (MDS) in one of the following categories: RCMD with an IPSS-R of intermediate, poor, or very poor, RAEB-1, or RAEB-2 Acute myeloid leukemia (AML) after first relapse or primary refractory disease Chronic myelogenous leukemia (CML) in Chronic Phase 2 or greater, Accelerated Phase or Blast Crisis Acute lymphoblastic leukemia (ALL) after first relapse or primary refractory disease, or High-Grade Non Hodgkin lymphoma (NHL) Stage III or IV after first relapse or primary refractory disease Hemophagocytic lymphohistiocytosis (HLH) Familial hemophagocytic lymphohistiocytosis (FLH) Viral-associated hemophagocytic syndrome (VAHS) T or NK cell lymphoproliferative syndrome X-linked lymphoproliferative disease (XLP) Inclusion Criteria at Time of T Cell Infusion: Engrafted with an absolute neutrophil count (ANC) > 500 cells/µL Greater than or equal to 50% donor chimerism in either peripheral blood or bone marrow, or relapse of their original disease Life expectancy > 30 days Lansky/Karnofsky score greater than or equal to 60 Absence of severe renal disease (creatinine > 2X upper limit of normal for age) Absence of severe hepatic disease (direct bilirubin > 3X upper limit of normal or SGOT > 3X upper limit of normal) Oxygen saturation > 94% on room air Patient/Guardian able to give informed consent AP1903 available in sufficient quantities to allow for treatment of the patient Exclusion Criteria: Exclusion Criteria at Time of T Cell Infusion: GvHD Severe intercurrent infection Pregnancy* Other investigational drugs in the prior 30 days Pregnancy test only required for at-risk individuals, defined as female patients of childbearing potential who have received a reduced-intensity conditioning regimen.
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Malcolm K Brenner, MB, PhD
Organizational Affiliation
Baylor College of Medicine
Official's Role
Principal Investigator
Facility Information:
Facility Name
Texas Children's Hospital
City
Houston
State/Province
Texas
ZIP/Postal Code
77030
Country
United States
Facility Name
The Methodist Hospital
City
Houston
State/Province
Texas
ZIP/Postal Code
77030
Country
United States

12. IPD Sharing Statement

Citations:
PubMed Identifier
25977584
Citation
Zhou X, Dotti G, Krance RA, Martinez CA, Naik S, Kamble RT, Durett AG, Dakhova O, Savoldo B, Di Stasi A, Spencer DM, Lin YF, Liu H, Grilley BJ, Gee AP, Rooney CM, Heslop HE, Brenner MK. Inducible caspase-9 suicide gene controls adverse effects from alloreplete T cells after haploidentical stem cell transplantation. Blood. 2015 Jun 25;125(26):4103-13. doi: 10.1182/blood-2015-02-628354. Epub 2015 May 14.
Results Reference
derived

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Administration of Donor T Cells With the Caspase-9 Suicide Gene

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