Analysis of Bacterial Microbiome of Endodontically Infected Primary and Permanent Teeth
Endodontic Disease, Endodontic Inflammation
About this trial
This is an interventional diagnostic trial for Endodontic Disease focused on measuring Endodontic disease, Microbiome, Permanent teeth, Primary teeth
Eligibility Criteria
Inclusion Criteria:
- have intact roots or <1/3 of physiological root resorption
- have clinical crowns that permit effective rubber dam isolation
- no mobility, fistula, pus discharge, gingival swelling, periapical abscess or internal resorption.
Exclusion Criteria:
- have marginal periodontitis, a history of pharmacological treatment, antibiotics or fluoride intake within the last 2 months
- a history of cancer, diabetes or immunodeficiency disorders
Sites / Locations
- Nuh Naci Yazgan Üniversitesi
Arms of the Study
Arm 1
Arm 2
Experimental
Experimental
Permanent teeth group
Primary teeth group
For the disinfection of teeth, 30% hydrogen peroxide and 2.5% sodium hypochlorite solution were used for 30 seconds each. Then, 5% sodium thiosulfate solution was used to inactivate the disinfectant agents. Cavity preparation and root canal access were accomplished using sterile high-speed diamond burs under water cooling. Microbial samples were taken immediately by the same researcher from the largest root canal under strict aseptic conditions by using paper point method. Sterilized minimum four paper points were placed to the same level in root canal and the root canal content was absorbed. Each paper point was kept into the canal for at least 30 seconds. Then, paper points were placed into the Eppendorf tubes and refrigerated at -80 °C within 10 min.
For the disinfection of teeth, 30% hydrogen peroxide and 2.5% sodium hypochlorite solution were used for 30 seconds each. Then, 5% sodium thiosulfate solution was used to inactivate the disinfectant agents. Cavity preparation and root canal access were accomplished using sterile high-speed diamond burs under water cooling. Microbial samples were taken immediately by the same researcher from the largest root canal under strict aseptic conditions by using paper point method. Sterilized minimum four paper points were placed to the same level in root canal and the root canal content was absorbed. Each paper point was kept into the canal for at least 30 seconds. Then, paper points were placed into the Eppendorf tubes and refrigerated at -80 °C within 10 min.