Back to resultsBaricitinib for Reduction of HIV - CNS
Primary Purpose
Human Immunodeficiency Virus
Status
Suspended
Phase
Phase 2
Locations
United States
Study Type
Interventional
Intervention
Baricitinib 2 MG Oral Tablet
Placebo
Sponsored by
About this trial
This is an interventional treatment trial for Human Immunodeficiency Virus focused on measuring Central Nervous System, Cerebrospinal Fluid, Baricitinib
Eligibility Criteria
Inclusion Criteria:
- HIV infected on continuous ART with plasma HIV RNA <200 copies/ml for at least 12 months (on at least two previous clinic visits and confirmed at screening).
- Current CD4+ > 350 cells/microliter for at least twelve months (on at least two previous clinic visits and confirmed at screening).
- Women of reproductive age will have a negative pregnancy test at study entry and agree to contraception while on study drug. Women who are at least 50 years of age and who have been amenorrheic for at least 12 months will not be required to agree to contraception to participate.
Exclusion Criteria:
- < 18 years of age or > 65 years of age
- Pregnancy or breastfeeding
- Significant hematological abnormalities at screening (ANC < 1500, Hgb<10, platelet< 100,000)
- History of progressive multifocal leukoencephalopathy
- Untreated latent tuberculosis infection (which will be screened for prior to entry)
- Immunosuppressive medications (including corticosteroids) and anticoagulants (aspirin acceptable)
- History of deep venous thrombosis
Cardiovascular disease:
- Coronary artery disease or history of myocardial infarction
- Congestive heart failure with left ventricular ejection fraction ≤40% per American Heart Association guidelines75
- Stroke history
- Hematologic malignancies including lymphoma and leukemia
- Major surgery within 8 weeks prior to screening or will require major surgery during the study
- Current or recent (<4 weeks prior to randomization) clinically serious viral (including COVID-19), bacterial, fungal, or parasitic infection or any other active or recent infection
- Symptomatic herpes simplex at the time of randomization
- Symptomatic herpes zoster infection within 12 weeks prior to randomization.
- History of disseminated/complicated herpes zoster (for example, ophthalmic zoster or CNS involvement).
Positive test for hepatitis B virus (HBV) defined as:
- positive for hepatitis B surface antigen (HBsAg), or
- positive for hepatitis B core antibody (HBcAb) and positive for hepatitis B virus deoxyribonucleic acid (HBV DNA)
- Hepatitis C virus (HCV) infection (hepatitis C antibody-positive and HCV ribonucleic acid [RNA]-positive).
- Cirrhosis of the liver from any cause
Any of the following specific abnormalities on screening laboratory tests:
- ALT or AST >2 x upper limits of normal (ULN)
- alkaline phosphatase (ALP) ≥2 x ULN
- total bilirubin ≥1.5 x ULN (with the exception of patients on atazanavir, who must have total bilirubin <2 x ULN)
- Chronic kidney disease with eGFR <40 mL/min/1.73 m2 (note that dose of baricitinib will be reduced to 1 mg daily in participants with GFR between 40 and 60).
- Current dependence on illicit drugs except for marijuana
Population: The study team will not include any of the following groups:
- Adults unable to consent
- Individuals who are not yet adults (infants, children, teenagers)
- Pregnant women
- Prisoners
- Cognitively impaired or Individuals with Impaired Decision-Making Capacity
- Individuals who are not able to clearly understand English
- Community Participation (if applicable)
Sites / Locations
- Grady Memorial Hospital
- Emory University Hospital
Arms of the Study
Arm 1
Arm 2
Arm Type
Experimental
Placebo Comparator
Arm Label
Baricitinib
Placebo
Arm Description
Potential participants will be pre-screened through review of the electronic medical record from Emory or Grady. Or if a potential participant receives care elsewhere, a release of medical information form will be signed and sent to the medical center that the individual goes to. The study team will enroll individuals who have well controlled HIV. Participants will then be randomized to either baricitinib or placebo. Patients randomized to Baricitinib group will receive Baricitinib at dose of 2 mg oral for ten weeks. Follow up visits will happen for both groups at weeks 1, 2, 4 and 10.
Potential participants will be pre-screened through review of the electronic medical record from Emory or Grady. Or if a potential participant receives care elsewhere, a release of medical information form will be signed and sent to the medical center that the individual goes to. The study team will enroll individuals who have well controlled HIV. Participants will then be randomized to either baricitinib or placebo. Patients randomized to the placebo group will receive 2 mg oral daily placebo for ten weeks. Follow up visits will happen for both groups at weeks 1, 2, 4 and 10.
Outcomes
Primary Outcome Measures
Changes in CSF HIV cell associated RNA by Double-R assay
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Briefly, after isolation of Peripheral blood mononuclear cells (PBMC) and cells from CSF, samples will be resuspended in phosphate buffered saline and stored for shipment in O-ring tubes. RNA will then be extracted using the Maxwell rapid sample concentrator (RSC) automated extraction platform. White Blood Cells (WBC) will be counted after red blood cell lysis using TC20 Automated Cell Counter, and these counts will be used to normalize the HIV-1 RNA results as copy numbers/106 cells. HIV-1 copy number per patient sample, in duplicate, is determined from a standard curve generated with HIV-1 plasmid controls, with a range of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Changes in CSF HIV cell associated CSF HIV DNA by Double-R assay
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Briefly, after isolation of Peripheral blood mononuclear cells (PBMC) and cells from CSF, samples will be resuspended in phosphate buffered saline and stored for shipment in O-ring tubes. DNA will then be extracted using the Maxwell rapid sample concentrator (RSC) automated extraction platform. WBCs will be counted after red blood cell lysis using TC20 Automated Cell Counter, and these counts will be used to normalize the HIV-1 DNA results as copy numbers/106 cells. HIV-1 copy number per patient sample, in duplicate, is determined from a standard curve generated with HIV-1 plasmid controls, with a range of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Changes in CSF cell associated DNA
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Cell associated DNA will be performed according to standard existing protocols using real-time PCR with a limit of detection of five copies.
Changes in CSF integrated proviral DNA
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
CSF integrated proviral DNA (IPDA and Alu-PCR) will be assessed: Integrated proviral DNA will be performed using existing methods in the clinical research group, which employ the Alu-PCR real-time platform, with a limit of 3 copies. Integrated proviral DNA assay (IPDA) to measure the replication competent reservoir will be used with digital droplet PCR methodology.
Changes in CSF HIV RNA by single copy assay
This method is based on a proprietary protocol which is used in conjunction with a commercial HIV-1 RNA easyQ reagent kit (bioMerieux Inc, Lyon, France). Briefly, a specimen of up to 2 ml of human plasma or CSF is added to lysis buffer containing guanidine thiocyanate. HIV-1 RNA is extracted in combination with the easyMAG platform (bioMerieux, Inc). Eluates containing HIV-1 RNA are aliquoted into 0.5 mL reaction tubes and amplified using 3 enzymes: T7 RNA polymerase, avian myeloblastosis virus reverse transcriptase, and RNase H. Molecular beacons targeting the pol/gag region of HIV-1 RNA are used for amplification and detection by isothermal reactions at 41oC. HIV-1 RNA level is quantified in conjunction with the NucliSENS easyQ HIV-1 v2.0 Director software and a proprietary algorithm developed by bioMONTR Labs. The dynamic range of this HIV-1 assay is 1-5,000,000 copies/mL of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Changes in HIV tat protein in CSF
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
HIV Tat in the CSF will be quantified according to published methods for ELISA to measure HIV Tat in human CSF.
Changes in HIV specific T cells in CSF
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Fresh PBMC and CSF cells will be cultured in the presence or absence of overlapping peptides which induces broad, non-specific T cell activation. The study team will measure cell surface markers by flow cytometry
Changes in CSF HIV antibodies by LIPS assay
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
CSF HIV antibodies by Luciferase Immunoprecipitation System(LIPS) assay (gp120, integrase, protease, and p24) will be assayed. The LIPS assay uses recombinant chimeric fusion proteins with luciferase producing light and specific antigens for quantitative detection of antibodies.
Changes in CSF CXCL10
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
The study team will measure CXCL10 in the setting of HIV using single molecule array technology by Quanterix.
Changes in CSF Neopterin
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
The study team will measure neopterin in the setting of HIV using the enzyme linked immunosorbent assays produced by Uman diagnostics, R&D Systems (ultra-sensitive), and Themo Fisher.
Changes in CSF IL-7
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
This will be measured using mesoscale discovery platform.
Changes in CSF IL-15
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
This will be measured using Mesoscale discovery platform
Changes in CSF Microtubule association protein-2
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF. This will be measured with ELISA.
Secondary Outcome Measures
Changes in Blood HIV cell associated RNA by Double-R assay
Changes in the blood between baseline and week ten after study medication. Briefly, after isolation of Peripheral blood mononuclear cells (PBMC) and cells from patient's blood sample, samples will be resuspended in phosphate buffered saline and stored for shipment in O-ring tubes. RNA will then be extracted using the Maxwell rapid sample concentrator (RSC) automated extraction platform. WBCs will be counted after red blood cell lysis using TC20 Automated Cell Counter, and these counts will be used to normalize the HIV-1 RNA results as copy numbers/106 cells. HIV-1 copy number per patient sample, in duplicate, is determined from a standard curve generated with HIV-1 plasmid controls, with a range of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Changes in Blood HIV cell associated DNA by Double-R assay
Changes in the blood between baseline and week ten after study medication. Briefly, after isolation of Peripheral blood mononuclear cells (PBMC) and cells from the patient's blood sample, samples will be resuspended in phosphate buffered saline and stored for shipment in O-ring tubes. DNA will then be extracted using the Maxwell rapid sample concentrator (RSC) automated extraction platform. WBCs will be counted after red blood cell lysis using TC20 Automated Cell Counter, and these counts will be used to normalize the HIV-1 DNA results as copy numbers/106 cells. HIV-1 copy number per patient sample, in duplicate, is determined from a standard curve generated with HIV-1 plasmid controls, with a range of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Changes in Blood cell associated DNA
Change in the blood between baseline and week ten after study medication. Cell associated DNA will be performed according to standard existing protocols using real-time PCR with a limit of detection of five copies.
Change in HIV DNA levels by Integrated proviral DNA assay
Change in the blood between baseline and week ten after study medication. The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Blood integrated proviral DNA (IPDA and Alu-PCR) will be assessed: Integrated proviral DNA will be performed using existing methods in our group, which employ the Alu-PCR real-time platform, with a limit of 3 copies. Integrated proviral DNA assay (IPDA) to measure the replication competent reservoir will be used with digital droplet PCR methodology
Changes in Blood HIV RNA by single copy assay
This method is based on a proprietary protocol which is used in conjunction with a commercial HIV-1 RNA easyQ reagent kit (bioMerieux Inc, Lyon, France). Briefly, a specimen of up to 2 ml of human plasma or CSF is added to lysis buffer containing guanidine thiocyanate. HIV-1 RNA is extracted in combination with the easyMAG platform (bioMerieux, Inc). Eluates containing HIV-1 RNA are aliquoted into 0.5 mL reaction tubes and amplified using 3 enzymes: T7 RNA polymerase, avian myeloblastosis virus reverse transcriptase, and RNase H. Molecular beacons targeting the pol/gag region of HIV-1 RNA are used for amplification and detection by isothermal reactions at 41oC. HIV-1 RNA level is quantified in conjunction with the NucliSENS easyQ HIV-1 v2.0 Director software and a proprietary algorithm developed by bioMONTR Labs. The dynamic range of this HIV-1 assay is 1-5,000,000 copies/mLof 0.73
Changes in Blood indirect viral markers: anti-HIV antibody levels
Change in HIV and inflammation levels in the blood between baseline and week ten after study medication.
Blood HIV antibodies by LIPS assay (gp120, integrase, protease, and p24) will be assayed. The LIPS assay uses recombinant chimeric fusion proteins with luciferase producing light and specific antigens for quantitative detection of antibodies.
Changes in Blood indirect viral markers: HIV specific T cells
Change in HIV and inflammation levels in the blood between baseline and week ten after study medication Fresh Human peripheral blood mononuclear cells (PBMC) will be cultured in the presence or absence of overlapping peptides which induces broad, non-specific T cell activation. In addition to cytokine production (focusing on TNFα, IFNγ, and IL-6 measured with quanterix technology) we will be measure cell surface markers by flow cytometry.
Changes in Blood CXCL10
Change in levels in the blood between baseline and week ten after study medication.
The study team will measure CXCL10 from participants blood samples, in the setting of HIV using single molecule array technology by Quanterix.
Changes in Blood Neopterin
Change in levels in the blood between baseline and week ten after study medication.
The study team will measure neopterin in the setting of HIV using the enzyme linked immunosorbent assays produced by Uman diagnostics, R&D Systems (ultra-sensitive), and Themo Fisher.
Changes in Blood IL-7
Change in levels in the blood between baseline and week ten after study medication.
This will be measured using mesoscale discovery platform.
Changes in Blood IL-15
Change in levels in the blood between baseline and week ten after study medication.
This will be measured using mesoscale discovery platform.
Changes in Stroop Test (Color Naming and Color-Word)
In psychology, the Stroop effect is the delay in reaction time between congruent and incongruent stimuli.
The effect has been used to create a psychological test (the Stroop test) that is widely used in clinical practice and investigation.
A basic task that demonstrates this effect occurs when there is a mismatch between the name of a color (e.g., "blue", "green", or "red") and the color it is printed on (i.e., the word "red" printed in blue ink instead of red ink). When asked to name the color of the word it takes longer and is more prone to errors when the color of the ink does not match the name of the color.
Word, color, and color-word T-Scores of 40 or less are considered "low." Word, color, and color-word T-Scores above 40 or are considered "normal." In order for one score to be considered "higher" or "lower" than another, a 10 point or greater T score difference is required.
Changes in Hopkins Verbal Learning Test II (Learning and Recall)
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
The Hopkins Verbal Learning Test-Revised (HVLT-R) is used to assess acquisition and delayed recall. The HVLT-R is a list learning test, which consists of 12 nouns within three semantic groups. The acquisition variable consists of three acquisition trials in which the administrator reads the words aloud and then asks the child to repeat as many as he/she can remember in any order. A delayed recall trial is introduced after 20-25 min, in which the child is asked to simply retrieve as many of the words listed in the acquisition trial as he/she can remember. The children are not informed about the delayed recall trial beforehand. Lower raw scores indicate difficulties with the task.
Changes in Brief Visuospatial Test (Learning and Recall)
The Brief Visuospatial Memory Test (BVMT) is a commonly used assessment tool to measure visuospatial learning and memory abilities.
In three Learning Trials, the respondent views the stimulus page for 10 seconds and is asked to draw as many of the figures as possible in their correct location on a page in the response booklet. A Delayed Recall Trial is administered after a 25-minute delay. Last, a Recognition Trial, in which the respondent is asked to identify which of 12 figures were included among the original geometric figures, is administered.
An optional Copy Trial may be administered to screen for severe visuoconstructive deficits and to help in scoring recall responses.
12 scores may be derived from BVMT-R performance. Recall performance is recorded for each of the immediate recall trials (Trial 1, Trail 2, and Trial 3) and for the delayed recall trial (Delayed Recall).
The recall scores are combined to form three additional summary measures of learning and memory.
Changes in Trailmaking
The Trail Making Test is a neuropsychological test of visual attention and task switching. It requires a subject to connect a sequence of 25 consecutive targets on a sheet of paper or computer screen. There are 2 parts to the test: in the first, the targets are all numbers from 1 to 25 and the test taker needs to connect them in sequential order; in the second part, the dots go from 1 to 13 and include letters from A to L. As in the first part, the patient must connect the dots in order while alternating letters and numbers, as in 1-A-2-B-3-C..., in the shortest time possible without lifting the pen from the paper. If the subject makes an error, the administrator corrects them before moving on to the next dot.
The subject's goal is to finish both parts as quickly as possible, with the time taken to complete the test being used as the primary performance metric. Scoring is based on time taken to complete the test (e.g. 35 seconds yielding a score of 35) with lower scores being better
Changes in Digit Symbol Test
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
The Digit Symbol Substitution Test (DSST) is a paper-and-pencil cognitive test presented on a single sheet of paper that requires a subject to match symbols to numbers according to a key located on the top of the page. The subject copies the symbol into spaces below a row of numbers. The number of correct symbols within the allowed time, usually 90 to 120 seconds, constitutes the score.
Changes in Letter Number Sequencing Test
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
Letter-number sequencing is a subtest of the WAIS (Wechsler Adult Intelligence Scale) and the WISC (Wechsler Intelligence Scale for Children). It is a test that measures an individual's short-term memory skills in being able to process and re-sequence information. The task involves hearing a series of letters and digits, and then reporting back the stimuli with the letters in alphabetical order, and digits in ascending numerical order. The task is traditionally administered orally, but recent studies have administered versions of the tasks visually by displaying stimuli on a computer screen. Results suggest that performance differences on the Letter-Number Sequencing task may arise as a function of language background and task administration modality
Changes in Wisconsin Card Sorting Test
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
Changes in Grooved Pegboard Test
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
The Grooved Pegboard Test (GPT) is used primarily as a measure of motor functioning, but some research indicates that performance on this test my also reflect cognitive factors, particularly attention and executive functioning.
This manipulative dexterity test contains twenty-five holes with randomly positioned slots and pegs which have a key along one side. Pegs must be rotated to match the hole before they can be inserted.
This procedure measures performance speed in a fine motor task by examining both sides of the body, inferences may be drawn regarding possible lateral brain damage.
The test requires more complete visual motor coordination than most of our pegboards and has been used in several neuropsychological test batteries, in student labs, and as a screening technique in industrial environment.
Changes in Letter and category fluency test
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
Changes in Beck Depression Inventory II (BDI-II)
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided. The BDI-II is a widely used 21-item self-report inventory measuring the severity of depression in adolescents and adults.
The questionnaire consists of 21 questions with answers ranging from 0 to 3. Scores are later analyzed by adding all scores and comparing to ranges :
0-10:These ups and downs are considered normal 11-16:Mild mood disturbance 17-20:Borderline clinical depression 21-30:Moderate depression 31-40: Severe depression over 4: Extreme depression
Changes in MRI neuroimaging findings
This will be performed with established techniques to evaluate brain structure and function.
Briefly, magnetic resonance (MR) images will be acquired on a 3T Siemens Magnetom Prisma-Fit scanner with a 32-channel receiver array head coil. Blood oxygenation level dependent (BOLD) contrast rsfMRI scans will be acquired using a simultaneous multi-slice (SMS) gradient echo (GE) echo planar imaging (EPI) sequence.
Changes in magnetic resonance spectroscopy (MRS) neuroimaging findings
This will be performed with established techniques to evaluate brain structure and function.
Spectra will be collected in frontal gray and white matter and in the basal ganglia for metabolites. Additionally, whole brain MRS will be acquired using whole brain echo planar spectroscopic imaging (EPSI).
Full Information
NCT ID
NCT05452564
First Posted
July 6, 2022
Last Updated
September 19, 2023
Sponsor
William Tyor
Collaborators
National Institute of Mental Health (NIMH)
1. Study Identification
Unique Protocol Identification Number
NCT05452564
Brief Title
Baricitinib for Reduction of HIV - CNS
Official Title
Phase II Study to Evaluate the Efficacy and Safety of Baricitinib for Reduction of HIV in the Central Nervous System
Study Type
Interventional
2. Study Status
Record Verification Date
September 2023
Overall Recruitment Status
Suspended
Why Stopped
Study is temporarily suspended pending a new IND application being requested by the FDA.
Study Start Date
May 18, 2023 (Actual)
Primary Completion Date
January 2028 (Anticipated)
Study Completion Date
January 2028 (Anticipated)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Sponsor-Investigator
Name of the Sponsor
William Tyor
Collaborators
National Institute of Mental Health (NIMH)
4. Oversight
Studies a U.S. FDA-regulated Drug Product
Yes
Studies a U.S. FDA-regulated Device Product
No
Data Monitoring Committee
Yes
5. Study Description
Brief Summary
There is still no cure for the human immunodeficiency virus (HIV). While combination antiretroviral therapy (cART) is effective in decreasing deaths from HIV, infected individuals face a lifetime of treatment and many potential complications including end organ diseases such as HIV-associated neurocognitive disorders. HIV infection is controllable with antiretroviral therapy (ART), but ART cannot eliminate HIV reservoirs. Thus, there is no available cure for HIV. There is a large and growing body of evidence that the central nervous system (CNS) is an HIV reservoir site and a barrier to HIV eradication. Our group has done extensive pre-clinical work with janus-kinase (JAK 1/2) inhibitors. This includes baricitinib, which is an orally available, FDA-approved drug for rheumatoid arthritis. Evidence suggests that this drug has activity against HIV in the central nervous system (CNS). In our recently completed pilot study, we showed that baricitinib crosses the blood brain barrier (BBB) and decreases HIV CNS persistence in the brain.
Using bloodwork, neurocognitive testing, MRIs and lumbar punctures, we plan to evaluate the change in central nervous system HIV after treatment with baricitinib versus placebo. We will also evaluate changes in neuroimaging, inflammation in blood and cerebrospinal fluid (CSF), and neuropsychological performance after treatment with baricitinib versus placebo.
Evidence shows that the central nervous system is one of the reservoir sites that enables the HIV virus to persist in the body even after years of treatment. In order to attack this reservoir and eventually find a cure, it is vital to learn if certain medications can suppress HIV in the CNS.
Detailed Description
HIV infection is controllable with antiretroviral therapy (ART), but ART cannot eliminate HIV reservoirs. Thus, there is no available cure for HIV. There is a large and growing body of evidence that the central nervous system (CNS) is an HIV reservoir site and a barrier to HIV eradication. HIV DNA is commonly found in brain from people with HIV (PWH) on suppressive ART. HIV genetic compartmentalization occurs in both the brain and CSF, showing that CNS HIV is distinct from peripheral sources such as blood. Multiple studies have demonstrated that CSF HIV RNA can be detected at very low levels during suppressive ART. A study from the AIDS Clinical Trials Group (ACTG) found HIV DNA in CSF cells from approximately 50% of PWH on suppressive ART, a finding that was associated with neurocognitive impairment. The study team's group recently demonstrated a significant advance in quantitating HIV from the CSF. Specifically, CSF cell associated HIV RNA was quantifiable in 90% and CSF HIV DNA was quantifiable in 80% of PWH on suppressive ART. Based on these multiple lines of evidence that the CNS is an HIV reservoir, more research is needed on therapies that have the potential to target the CNS reservoir.
The study team has performed extensive pre-clinical and clinical work on the Janus Kinase (Jak 1/2) inhibitor drug class for viral infections. This includes work on ruxolitinib and baricitinib, two FDA approved orally bioavailable agents for myelofibrosis and polycythemia vera (ruxolitinib) as well as rheumatoid arthritis (baricitinib). The investigators have evaluated their potential to target HIV in the CNS in vitro, ex vivo, and in vivo Specifically, the study team has demonstrated that these agents block HIV replication and infection in key CNS cells, reduce the lifespan of HIV CNS reservoirs, and block reservoir reseeding. In vivo, the study team has shown in the murine model that baricitinib decreases CNS HIV and reverses behavioral abnormalities associated with HIV, which correlates with reversal of phenotypic markers of brain inflammation. This has also demonstrated that baricitinib reaches therapeutic CNS concentrations in the rhesus macaque model (including in brain parenchyma) as well as in humans. In a recently published multi-site Phase 2a ACTG-sponsored study with ruxolitinib (A5336, n = 60),the research group demonstrated that ruxolitinib is safe, well-tolerated, and reduces key markers of immune activation. New data that ruxolitinib decreased the peripheral HIV reservoir in a subset of A5336 participants provides even more evidence for this drug class to be included in eradication strategies. However, A5336 did not have any CNS assessments. The investigators now propose to study baricitinib, one of the most promising Jak 1/2 inhibitors with once daily dosing, renal clearance, and a more favorable safety/ pharmacokinetic profile, as a therapy to decrease the HIV CNS reservoir in PWH with durable virologic suppression on ART.
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Human Immunodeficiency Virus
Keywords
Central Nervous System, Cerebrospinal Fluid, Baricitinib
7. Study Design
Primary Purpose
Treatment
Study Phase
Phase 2
Interventional Study Model
Parallel Assignment
Masking
ParticipantCare ProviderInvestigator
Allocation
Randomized
Enrollment
95 (Anticipated)
8. Arms, Groups, and Interventions
Arm Title
Baricitinib
Arm Type
Experimental
Arm Description
Potential participants will be pre-screened through review of the electronic medical record from Emory or Grady. Or if a potential participant receives care elsewhere, a release of medical information form will be signed and sent to the medical center that the individual goes to. The study team will enroll individuals who have well controlled HIV. Participants will then be randomized to either baricitinib or placebo.
Patients randomized to Baricitinib group will receive Baricitinib at dose of 2 mg oral for ten weeks. Follow up visits will happen for both groups at weeks 1, 2, 4 and 10.
Arm Title
Placebo
Arm Type
Placebo Comparator
Arm Description
Potential participants will be pre-screened through review of the electronic medical record from Emory or Grady. Or if a potential participant receives care elsewhere, a release of medical information form will be signed and sent to the medical center that the individual goes to. The study team will enroll individuals who have well controlled HIV. Participants will then be randomized to either baricitinib or placebo.
Patients randomized to the placebo group will receive 2 mg oral daily placebo for ten weeks. Follow up visits will happen for both groups at weeks 1, 2, 4 and 10.
Intervention Type
Drug
Intervention Name(s)
Baricitinib 2 MG Oral Tablet
Other Intervention Name(s)
Baricitinib
Intervention Description
Baricitinib, a Janus Kinase inhibitor drug for viral infections, will be administered orally to subjects randomized to this intervention. The dose will be 2 mg orally for ten weeks. This will be compared with placebo intervention. Follow up visits will take place at week 1,2,4 and 10.
Intervention Type
Drug
Intervention Name(s)
Placebo
Intervention Description
Patients randomized to the placebo group will receive 2 mg oral daily placebo for ten weeks. Follow up visits will happen for both groups at weeks 1, 2, 4 and 10.
Primary Outcome Measure Information:
Title
Changes in CSF HIV cell associated RNA by Double-R assay
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Briefly, after isolation of Peripheral blood mononuclear cells (PBMC) and cells from CSF, samples will be resuspended in phosphate buffered saline and stored for shipment in O-ring tubes. RNA will then be extracted using the Maxwell rapid sample concentrator (RSC) automated extraction platform. White Blood Cells (WBC) will be counted after red blood cell lysis using TC20 Automated Cell Counter, and these counts will be used to normalize the HIV-1 RNA results as copy numbers/106 cells. HIV-1 copy number per patient sample, in duplicate, is determined from a standard curve generated with HIV-1 plasmid controls, with a range of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Time Frame
Baseline and study week 10
Title
Changes in CSF HIV cell associated CSF HIV DNA by Double-R assay
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Briefly, after isolation of Peripheral blood mononuclear cells (PBMC) and cells from CSF, samples will be resuspended in phosphate buffered saline and stored for shipment in O-ring tubes. DNA will then be extracted using the Maxwell rapid sample concentrator (RSC) automated extraction platform. WBCs will be counted after red blood cell lysis using TC20 Automated Cell Counter, and these counts will be used to normalize the HIV-1 DNA results as copy numbers/106 cells. HIV-1 copy number per patient sample, in duplicate, is determined from a standard curve generated with HIV-1 plasmid controls, with a range of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Time Frame
Baseline and study week 10
Title
Changes in CSF cell associated DNA
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Cell associated DNA will be performed according to standard existing protocols using real-time PCR with a limit of detection of five copies.
Time Frame
Baseline and study week 10
Title
Changes in CSF integrated proviral DNA
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
CSF integrated proviral DNA (IPDA and Alu-PCR) will be assessed: Integrated proviral DNA will be performed using existing methods in the clinical research group, which employ the Alu-PCR real-time platform, with a limit of 3 copies. Integrated proviral DNA assay (IPDA) to measure the replication competent reservoir will be used with digital droplet PCR methodology.
Time Frame
Baseline and study week 10
Title
Changes in CSF HIV RNA by single copy assay
Description
This method is based on a proprietary protocol which is used in conjunction with a commercial HIV-1 RNA easyQ reagent kit (bioMerieux Inc, Lyon, France). Briefly, a specimen of up to 2 ml of human plasma or CSF is added to lysis buffer containing guanidine thiocyanate. HIV-1 RNA is extracted in combination with the easyMAG platform (bioMerieux, Inc). Eluates containing HIV-1 RNA are aliquoted into 0.5 mL reaction tubes and amplified using 3 enzymes: T7 RNA polymerase, avian myeloblastosis virus reverse transcriptase, and RNase H. Molecular beacons targeting the pol/gag region of HIV-1 RNA are used for amplification and detection by isothermal reactions at 41oC. HIV-1 RNA level is quantified in conjunction with the NucliSENS easyQ HIV-1 v2.0 Director software and a proprietary algorithm developed by bioMONTR Labs. The dynamic range of this HIV-1 assay is 1-5,000,000 copies/mL of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Time Frame
Baseline and study week 10
Title
Changes in HIV tat protein in CSF
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
HIV Tat in the CSF will be quantified according to published methods for ELISA to measure HIV Tat in human CSF.
Time Frame
Baseline and study week 10
Title
Changes in HIV specific T cells in CSF
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Fresh PBMC and CSF cells will be cultured in the presence or absence of overlapping peptides which induces broad, non-specific T cell activation. The study team will measure cell surface markers by flow cytometry
Time Frame
Baseline and study week 10
Title
Changes in CSF HIV antibodies by LIPS assay
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
CSF HIV antibodies by Luciferase Immunoprecipitation System(LIPS) assay (gp120, integrase, protease, and p24) will be assayed. The LIPS assay uses recombinant chimeric fusion proteins with luciferase producing light and specific antigens for quantitative detection of antibodies.
Time Frame
Baseline and study week 10
Title
Changes in CSF CXCL10
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
The study team will measure CXCL10 in the setting of HIV using single molecule array technology by Quanterix.
Time Frame
Baseline and study week 10
Title
Changes in CSF Neopterin
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
The study team will measure neopterin in the setting of HIV using the enzyme linked immunosorbent assays produced by Uman diagnostics, R&D Systems (ultra-sensitive), and Themo Fisher.
Time Frame
Baseline and study week 10
Title
Changes in CSF IL-7
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
This will be measured using mesoscale discovery platform.
Time Frame
Baseline and study week 10
Title
Changes in CSF IL-15
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
This will be measured using Mesoscale discovery platform
Time Frame
Baseline and study week 10
Title
Changes in CSF Microtubule association protein-2
Description
The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF. This will be measured with ELISA.
Time Frame
Baseline and study week 10
Secondary Outcome Measure Information:
Title
Changes in Blood HIV cell associated RNA by Double-R assay
Description
Changes in the blood between baseline and week ten after study medication. Briefly, after isolation of Peripheral blood mononuclear cells (PBMC) and cells from patient's blood sample, samples will be resuspended in phosphate buffered saline and stored for shipment in O-ring tubes. RNA will then be extracted using the Maxwell rapid sample concentrator (RSC) automated extraction platform. WBCs will be counted after red blood cell lysis using TC20 Automated Cell Counter, and these counts will be used to normalize the HIV-1 RNA results as copy numbers/106 cells. HIV-1 copy number per patient sample, in duplicate, is determined from a standard curve generated with HIV-1 plasmid controls, with a range of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Time Frame
Baseline and study week 10
Title
Changes in Blood HIV cell associated DNA by Double-R assay
Description
Changes in the blood between baseline and week ten after study medication. Briefly, after isolation of Peripheral blood mononuclear cells (PBMC) and cells from the patient's blood sample, samples will be resuspended in phosphate buffered saline and stored for shipment in O-ring tubes. DNA will then be extracted using the Maxwell rapid sample concentrator (RSC) automated extraction platform. WBCs will be counted after red blood cell lysis using TC20 Automated Cell Counter, and these counts will be used to normalize the HIV-1 DNA results as copy numbers/106 cells. HIV-1 copy number per patient sample, in duplicate, is determined from a standard curve generated with HIV-1 plasmid controls, with a range of 0.73 - 1600 HIV-1 copies /μl. The HIV-1 copy number is normalized per one million of WBCs.
Time Frame
Baseline and study week 10
Title
Changes in Blood cell associated DNA
Description
Change in the blood between baseline and week ten after study medication. Cell associated DNA will be performed according to standard existing protocols using real-time PCR with a limit of detection of five copies.
Time Frame
Baseline and study week 10
Title
Change in HIV DNA levels by Integrated proviral DNA assay
Description
Change in the blood between baseline and week ten after study medication. The study team will obtain up to 40 milliliters (ml) of CSF from individuals who weigh at least 60 kilograms, otherwise we will draw up to 30 ml of CSF.
Blood integrated proviral DNA (IPDA and Alu-PCR) will be assessed: Integrated proviral DNA will be performed using existing methods in our group, which employ the Alu-PCR real-time platform, with a limit of 3 copies. Integrated proviral DNA assay (IPDA) to measure the replication competent reservoir will be used with digital droplet PCR methodology
Time Frame
Baseline and study week 10
Title
Changes in Blood HIV RNA by single copy assay
Description
This method is based on a proprietary protocol which is used in conjunction with a commercial HIV-1 RNA easyQ reagent kit (bioMerieux Inc, Lyon, France). Briefly, a specimen of up to 2 ml of human plasma or CSF is added to lysis buffer containing guanidine thiocyanate. HIV-1 RNA is extracted in combination with the easyMAG platform (bioMerieux, Inc). Eluates containing HIV-1 RNA are aliquoted into 0.5 mL reaction tubes and amplified using 3 enzymes: T7 RNA polymerase, avian myeloblastosis virus reverse transcriptase, and RNase H. Molecular beacons targeting the pol/gag region of HIV-1 RNA are used for amplification and detection by isothermal reactions at 41oC. HIV-1 RNA level is quantified in conjunction with the NucliSENS easyQ HIV-1 v2.0 Director software and a proprietary algorithm developed by bioMONTR Labs. The dynamic range of this HIV-1 assay is 1-5,000,000 copies/mLof 0.73
Time Frame
Baseline and study week 10
Title
Changes in Blood indirect viral markers: anti-HIV antibody levels
Description
Change in HIV and inflammation levels in the blood between baseline and week ten after study medication.
Blood HIV antibodies by LIPS assay (gp120, integrase, protease, and p24) will be assayed. The LIPS assay uses recombinant chimeric fusion proteins with luciferase producing light and specific antigens for quantitative detection of antibodies.
Time Frame
Baseline and study week 10
Title
Changes in Blood indirect viral markers: HIV specific T cells
Description
Change in HIV and inflammation levels in the blood between baseline and week ten after study medication Fresh Human peripheral blood mononuclear cells (PBMC) will be cultured in the presence or absence of overlapping peptides which induces broad, non-specific T cell activation. In addition to cytokine production (focusing on TNFα, IFNγ, and IL-6 measured with quanterix technology) we will be measure cell surface markers by flow cytometry.
Time Frame
Baseline and study week 10
Title
Changes in Blood CXCL10
Description
Change in levels in the blood between baseline and week ten after study medication.
The study team will measure CXCL10 from participants blood samples, in the setting of HIV using single molecule array technology by Quanterix.
Time Frame
Baseline and study week 10
Title
Changes in Blood Neopterin
Description
Change in levels in the blood between baseline and week ten after study medication.
The study team will measure neopterin in the setting of HIV using the enzyme linked immunosorbent assays produced by Uman diagnostics, R&D Systems (ultra-sensitive), and Themo Fisher.
Time Frame
Baseline and study week 10
Title
Changes in Blood IL-7
Description
Change in levels in the blood between baseline and week ten after study medication.
This will be measured using mesoscale discovery platform.
Time Frame
Baseline and study week 10
Title
Changes in Blood IL-15
Description
Change in levels in the blood between baseline and week ten after study medication.
This will be measured using mesoscale discovery platform.
Time Frame
Baseline and study week 10
Title
Changes in Stroop Test (Color Naming and Color-Word)
Description
In psychology, the Stroop effect is the delay in reaction time between congruent and incongruent stimuli.
The effect has been used to create a psychological test (the Stroop test) that is widely used in clinical practice and investigation.
A basic task that demonstrates this effect occurs when there is a mismatch between the name of a color (e.g., "blue", "green", or "red") and the color it is printed on (i.e., the word "red" printed in blue ink instead of red ink). When asked to name the color of the word it takes longer and is more prone to errors when the color of the ink does not match the name of the color.
Word, color, and color-word T-Scores of 40 or less are considered "low." Word, color, and color-word T-Scores above 40 or are considered "normal." In order for one score to be considered "higher" or "lower" than another, a 10 point or greater T score difference is required.
Time Frame
Baseline and study week 10
Title
Changes in Hopkins Verbal Learning Test II (Learning and Recall)
Description
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
The Hopkins Verbal Learning Test-Revised (HVLT-R) is used to assess acquisition and delayed recall. The HVLT-R is a list learning test, which consists of 12 nouns within three semantic groups. The acquisition variable consists of three acquisition trials in which the administrator reads the words aloud and then asks the child to repeat as many as he/she can remember in any order. A delayed recall trial is introduced after 20-25 min, in which the child is asked to simply retrieve as many of the words listed in the acquisition trial as he/she can remember. The children are not informed about the delayed recall trial beforehand. Lower raw scores indicate difficulties with the task.
Time Frame
Baseline and study week 10
Title
Changes in Brief Visuospatial Test (Learning and Recall)
Description
The Brief Visuospatial Memory Test (BVMT) is a commonly used assessment tool to measure visuospatial learning and memory abilities.
In three Learning Trials, the respondent views the stimulus page for 10 seconds and is asked to draw as many of the figures as possible in their correct location on a page in the response booklet. A Delayed Recall Trial is administered after a 25-minute delay. Last, a Recognition Trial, in which the respondent is asked to identify which of 12 figures were included among the original geometric figures, is administered.
An optional Copy Trial may be administered to screen for severe visuoconstructive deficits and to help in scoring recall responses.
12 scores may be derived from BVMT-R performance. Recall performance is recorded for each of the immediate recall trials (Trial 1, Trail 2, and Trial 3) and for the delayed recall trial (Delayed Recall).
The recall scores are combined to form three additional summary measures of learning and memory.
Time Frame
Baseline and study week 10
Title
Changes in Trailmaking
Description
The Trail Making Test is a neuropsychological test of visual attention and task switching. It requires a subject to connect a sequence of 25 consecutive targets on a sheet of paper or computer screen. There are 2 parts to the test: in the first, the targets are all numbers from 1 to 25 and the test taker needs to connect them in sequential order; in the second part, the dots go from 1 to 13 and include letters from A to L. As in the first part, the patient must connect the dots in order while alternating letters and numbers, as in 1-A-2-B-3-C..., in the shortest time possible without lifting the pen from the paper. If the subject makes an error, the administrator corrects them before moving on to the next dot.
The subject's goal is to finish both parts as quickly as possible, with the time taken to complete the test being used as the primary performance metric. Scoring is based on time taken to complete the test (e.g. 35 seconds yielding a score of 35) with lower scores being better
Time Frame
Baseline and study week 10
Title
Changes in Digit Symbol Test
Description
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
The Digit Symbol Substitution Test (DSST) is a paper-and-pencil cognitive test presented on a single sheet of paper that requires a subject to match symbols to numbers according to a key located on the top of the page. The subject copies the symbol into spaces below a row of numbers. The number of correct symbols within the allowed time, usually 90 to 120 seconds, constitutes the score.
Time Frame
Baseline and study week 10
Title
Changes in Letter Number Sequencing Test
Description
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
Letter-number sequencing is a subtest of the WAIS (Wechsler Adult Intelligence Scale) and the WISC (Wechsler Intelligence Scale for Children). It is a test that measures an individual's short-term memory skills in being able to process and re-sequence information. The task involves hearing a series of letters and digits, and then reporting back the stimuli with the letters in alphabetical order, and digits in ascending numerical order. The task is traditionally administered orally, but recent studies have administered versions of the tasks visually by displaying stimuli on a computer screen. Results suggest that performance differences on the Letter-Number Sequencing task may arise as a function of language background and task administration modality
Time Frame
Baseline and study week 10
Title
Changes in Wisconsin Card Sorting Test
Description
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
Time Frame
Baseline and study week 10
Title
Changes in Grooved Pegboard Test
Description
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
The Grooved Pegboard Test (GPT) is used primarily as a measure of motor functioning, but some research indicates that performance on this test my also reflect cognitive factors, particularly attention and executive functioning.
This manipulative dexterity test contains twenty-five holes with randomly positioned slots and pegs which have a key along one side. Pegs must be rotated to match the hole before they can be inserted.
This procedure measures performance speed in a fine motor task by examining both sides of the body, inferences may be drawn regarding possible lateral brain damage.
The test requires more complete visual motor coordination than most of our pegboards and has been used in several neuropsychological test batteries, in student labs, and as a screening technique in industrial environment.
Time Frame
Baseline and study week 10
Title
Changes in Letter and category fluency test
Description
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided.
Time Frame
Baseline and study week 10
Title
Changes in Beck Depression Inventory II (BDI-II)
Description
This will be performed in a quiet exam room. Participants will have the opportunity to take breaks as needed and snacks will be provided. The BDI-II is a widely used 21-item self-report inventory measuring the severity of depression in adolescents and adults.
The questionnaire consists of 21 questions with answers ranging from 0 to 3. Scores are later analyzed by adding all scores and comparing to ranges :
0-10:These ups and downs are considered normal 11-16:Mild mood disturbance 17-20:Borderline clinical depression 21-30:Moderate depression 31-40: Severe depression over 4: Extreme depression
Time Frame
Baseline and study week 10
Title
Changes in MRI neuroimaging findings
Description
This will be performed with established techniques to evaluate brain structure and function.
Briefly, magnetic resonance (MR) images will be acquired on a 3T Siemens Magnetom Prisma-Fit scanner with a 32-channel receiver array head coil. Blood oxygenation level dependent (BOLD) contrast rsfMRI scans will be acquired using a simultaneous multi-slice (SMS) gradient echo (GE) echo planar imaging (EPI) sequence.
Time Frame
Baseline and study week 10
Title
Changes in magnetic resonance spectroscopy (MRS) neuroimaging findings
Description
This will be performed with established techniques to evaluate brain structure and function.
Spectra will be collected in frontal gray and white matter and in the basal ganglia for metabolites. Additionally, whole brain MRS will be acquired using whole brain echo planar spectroscopic imaging (EPSI).
Time Frame
Baseline and study week 10
10. Eligibility
Sex
All
Minimum Age & Unit of Time
18 Years
Maximum Age & Unit of Time
65 Years
Accepts Healthy Volunteers
No
Eligibility Criteria
Inclusion Criteria:
HIV infected on continuous ART with plasma HIV RNA <200 copies/ml for at least 12 months (on at least two previous clinic visits and confirmed at screening).
Current CD4+ > 350 cells/microliter for at least twelve months (on at least two previous clinic visits and confirmed at screening).
Women of reproductive age will have a negative pregnancy test at study entry and agree to contraception while on study drug. Women who are at least 50 years of age and who have been amenorrheic for at least 12 months will not be required to agree to contraception to participate.
Exclusion Criteria:
< 18 years of age or > 65 years of age
Pregnancy or breastfeeding
Significant hematological abnormalities at screening (ANC < 1500, Hgb<10, platelet< 100,000)
History of progressive multifocal leukoencephalopathy
Untreated latent tuberculosis infection (which will be screened for prior to entry)
Immunosuppressive medications (including corticosteroids) and anticoagulants (aspirin acceptable)
History of deep venous thrombosis
Cardiovascular disease:
Coronary artery disease or history of myocardial infarction
Congestive heart failure with left ventricular ejection fraction ≤40% per American Heart Association guidelines75
Stroke history
Hematologic malignancies including lymphoma and leukemia
Major surgery within 8 weeks prior to screening or will require major surgery during the study
Current or recent (<4 weeks prior to randomization) clinically serious viral (including COVID-19), bacterial, fungal, or parasitic infection or any other active or recent infection
Symptomatic herpes simplex at the time of randomization
Symptomatic herpes zoster infection within 12 weeks prior to randomization.
History of disseminated/complicated herpes zoster (for example, ophthalmic zoster or CNS involvement).
Positive test for hepatitis B virus (HBV) defined as:
positive for hepatitis B surface antigen (HBsAg), or
positive for hepatitis B core antibody (HBcAb) and positive for hepatitis B virus deoxyribonucleic acid (HBV DNA)
Hepatitis C virus (HCV) infection (hepatitis C antibody-positive and HCV ribonucleic acid [RNA]-positive).
Cirrhosis of the liver from any cause
Any of the following specific abnormalities on screening laboratory tests:
ALT or AST >2 x upper limits of normal (ULN)
alkaline phosphatase (ALP) ≥2 x ULN
total bilirubin ≥1.5 x ULN (with the exception of patients on atazanavir, who must have total bilirubin <2 x ULN)
Chronic kidney disease with eGFR <40 mL/min/1.73 m2 (note that dose of baricitinib will be reduced to 1 mg daily in participants with GFR between 40 and 60).
Current dependence on illicit drugs except for marijuana
Population: The study team will not include any of the following groups:
Adults unable to consent
Individuals who are not yet adults (infants, children, teenagers)
Pregnant women
Prisoners
Cognitively impaired or Individuals with Impaired Decision-Making Capacity
Individuals who are not able to clearly understand English
Community Participation (if applicable)
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
William Tyor, MD
Organizational Affiliation
Professor
Official's Role
Principal Investigator
Facility Information:
Facility Name
Grady Memorial Hospital
City
Atlanta
State/Province
Georgia
ZIP/Postal Code
30303
Country
United States
Facility Name
Emory University Hospital
City
Atlanta
State/Province
Georgia
ZIP/Postal Code
30322
Country
United States
12. IPD Sharing Statement
Plan to Share IPD
Yes
IPD Sharing Plan Description
Medical and social history, laboratory results, imaging results, neuropsychological test results
IPD Sharing Time Frame
At the end of the study until 2027
IPD Sharing Access Criteria
Qualified investigators for secondary analyses not related to the primary aims of the study following Emory University guidelines
Learn more about this trial
Baricitinib for Reduction of HIV - CNS
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