Carcinoembryonic Antigen-loaded Dendritic Cells in Advanced Colorectal Cancer Patients

For arm A and B Inclusion Criteria: Histological documented evidence of colorectal cancer. Primary tumor surgically removed, recurrence(s) in the liver. Planned surgical excision of liver metastases. HLA-A2.1 phenotype according to lymphocyte HLA typing. Expression of CEA on primary tumor. ECOG performance status 0-1, life expectancy > 3 months. Age 18-75 years. WBC > 3.0 x 109/l, lymphocytes > 0.8 x 109/l, platelets > 100 x 109/l, serum creatinine < 150 μmol/l, serum bilirubin < 25 μmol/l. Expected adequacy of follow-up. Written informed consent. Exclusion Criteria: Clinical signs of extra hepatic metastases, in patients with a clinical suspicion of other metastases diagnostic tests should be performed to exclude this. Prior chemotherapy, immunotherapy, or radiotherapy within three months before planned surgical excision is allowed. A history of myocardial infarction, angina pectoris, cardiac arrhythmias, cerebrovascular accidents, transient ischemic attacks or severe hypertension (exclusion criteria for autologous blood donation) Concomitant use of corticosteroids or other immunosuppressive agents. A history of any second malignancy in the past five years excluding adequately treated basal carcinoma of skin or carcinoma in situ of cervix. Serious concomitant disease, active infections. Specifically, patients with autoimmune disease or organ allografts and patients with a history of HBsAg or HIV are excluded. A known allergy to shell fish. Pregnant or lactating women. For arm C (side-study) inclusion criteria: histological proof of colorectal cancer HLA-A0201 positive stage III (T1-4N1-2M0) cancer or high risk stage II (T4 and/or poor differentiation in histology and/or perforation and/or obstruction and/or venous invasion and/or histological analysis of ≤10 lymph nodes) ≤ 8 weeks since surgical resection of primary colorectal tumor Age 18-75 years WHO performance 0-1 (Karnofsky 100-70%) WBC ≥ 3.0x109/l Platelets ≥ 100x109/l Hb ≥ 6 mmol/l Total bilirubin ≤ 2x UNL ASAT and ALAT ≤ 3x UNL Serum creatinine ≤ 1.5 x UNL Expected adequacy of follow-up Signed written informed consent exclusion criteria A history of second malignancy within the last 5 years. Adequately treated basal carcino¬ma of skin or carcinoma in situ of cervix is acceptable within this period Serious concomitant disease. Autoimmune disease or organ grafts. Other serious concomitant diseases preventing the safe administration of study drugs or likely to interfere with the study assessments. A known allergy to shell fish (contains KLH) Pregnant or lactating women