Clinical and Metagenomic Investigation of Antimicrobial Peptide Gel in Periodontal Treatment.
Periodontitis
About this trial
This is an interventional treatment trial for Periodontitis
Eligibility Criteria
Inclusion Criteria:
• Subject diagnosed with generalised periodontitis Stage III of any grade (Caton et al., 2018).
- Age ≥21 years old.
- Subject is in good general health without any remarkable past history of disease except for well controlled diabetes (HbA1c ≤6.5%) (Clinical practice guidelines on management of type 2 diabetes mellitus., 2020).
- Presence of at least 20 teeth.
- Presence of at least 2 non-adjacent sites located in contralateral quadrant with interproximal probing pocket depth (PPD) ≥6mm.
Exclusion Criteria:
• Received subgingival scaling in the previous 6 months.
- Use of antibiotic in the past 6 months.
- Taking steroidal and non-steroidal anti-inflammatory agents on a daily basis.
- Medical condition requiring antibiotic prophylaxis.
- Pregnancy or lactating.
- Smoking ≥10 cigarettes per day.
Sites / Locations
- faculty of dentistry, University of Malaya
Arms of the Study
Arm 1
Arm 2
Experimental
Sham Comparator
test group (antimicrobial peptide)
control group (normal saline)
Half of the dentition will receive scaling and root surface debridement within a week followed by the application of antimicrobial peptide gel Ace Helper™ into the site with pocket depth >5mm until overflow noticed. Antimicrobial peptide (AMP) will be reapplied for 2 more times at an interval of 3 days. Antimicrobial peptide gel Ace Helper™ contained 0.85% synthetic AMP (TAPS-18) designed based on the basic structure of cathelicidin, hydroxyethyl cellulose, and purified water.
Half of the dentition will receive scaling and root surface debridement within a week followed by the irrigation of 0.9% normal saline into the site with pocket depth >5mm. Normal saline irrigation will be repeated for 2 more times at an interval of 3 days.