Comparison of Biofilm Characteristics Between an in Situ Biofilm Model of Supragingival Plaque and Natural Teeth
Biofilm, Dental Plaque
About this trial
This is an interventional basic science trial for Biofilm focused on measuring pyrosequencing, 16S DNA gene, Confocal Laser Scanning Microscope, bacterial viability, bacterial diversity
Eligibility Criteria
Inclusion Criteria:
- systemically healthy
- good oral health status
Exclusion Criteria:
- smoker or former smoker,
- presence of dental prostheses or orthodontic devices,
- antibiotic treatment or routine use of oral antiseptics in the previous 3 months,
- presence of any systemic disease that could alter the production or composition of saliva
Sites / Locations
- Univeristy of Santiago de Compostela, Faculty of DentistryRecruiting
Arms of the Study
Arm 1
Arm 2
Arm 3
No Intervention
No Intervention
Active Comparator
No toothbrushing during experiment and Pyrosequencing
No toothbrushing during experiment
Toothbrushing during experiment
Volunteers will not brush their teeth during 48 hours. Dental plaque samples will be analyse by CLSM (confocal Laser scanning microscope) and 16S DNA pyrosequencing.
Volunteers will not brush their teeth during 48 hours. Dental plaque samples will be analyse by CLSM (confocal Laser scanning microscope).
48hours-Dental plaque samples will be analyse by CLSM (confocal Laser scanning microscope).