Culture Media Microdroplet Geometry With Different Oil Overlay and Its Effect on Embryonic Development.
Embryonic Development, Infertility
About this trial
This is an interventional other trial for Embryonic Development focused on measuring dry incubator, Osmolality, Oil overlay, Microdrops
Eligibility Criteria
Inclusion Criteria:
- Female age is ≤ 37 years old with ≥ 8 MII oocytes
- Males with normal semen parameters (WHO 2010).
- We conducted three experiments all with Siblings oocytes splits and all embryos
Exclusion Criteria:
- Oocyte or sperm donation.
- Sever male factor.
- Any contradictions to undergoing in vitro fertilization or goandotropin stimulation.
Sites / Locations
- Ganin Fertility Center
Arms of the Study
Arm 1
Arm 2
Arm 3
Arm 4
Other
Other
Other
Other
GPS / LM versus SPL/LM
GPS/PO versus SPL/PO
GPS/PO versus GPS/LM
SPL/PO versus SPL/LM
to determine the effect of micro droplet geometry in 3D dishes (GPS) or 2D dishes (SPL) in combination with light mineral oil (LM).
to determine the effect of micro droplet geometry in 3D dishes (GPS) or 2D dishes (SPL) in combination with paraffin oil (PO).
to determine the effect of oil overlay light mineral(LM) or paraffin oil (PO) in combination with paraffin oil 3D dishes (GPS).
to determine the effect of oil overlay light mineral(LM) or paraffin oil (PO) in combination with paraffin oil 2D dishes (SPL).