Cement flooRs AnD chiLd hEalth (CRADLE)

Prevalence of Ascaris lumbricoides, Necator americanus, or Trichuris trichiura infection in birth cohort detected with qPCR at 6, 12, 18, or 24 months follow-up

Prevalence of Ascaris lumbricoides infection in birth cohort detected with qPCR at 6, 12, 18, or 24 months follow-up

Prevalence of Necator americanus infection in birth cohort detected with qPCR at 6, 12, 18, or 24 months follow-up

Prevalence of Trichuris trichiura infection in birth cohort detected with qPCR at 6, 12, 18, or 24 months follow-up

Prevalence of diarrhea is defined as 3 or more loose or watery stools in 24 hours or 1 or more stools with blood in 24 hours at any follow-up measurement. Diarrhea will be based on caregiver-reported symptoms in the birth cohort with 2-day and 7-day recall at any follow-up measurement through month 24

Prevalence of other enteric pathogens in stool samples detected using a TaqMan array card in a sub-sample of the birth cohort

Mean Z-score using the Ages and Stages Questionnaire Inventory (ASQi) overall and within 5 domains: communication skills, gross motor skills, fine motor skills, problem solving skills, and personal social skills. Z-scores will be calculated for each domain that are standardized by age in days to the control group. Z-scores > 0 indicate child development attainment above the median for children of the same age in the control arm. Z-scores < 0 indicate child development attainment below the median for children of the same age in the control arm.

Length or height will be measured at birth, and 3, 6, 12, 18, and 24 months follow-up. Length-for-age z-scores (LAZ) will be calculated using the World Health Organization's 2006 Child Growth Standards. LAZ < -2 standard distributions below the median is defined as moderately stunted, and LAZ < -3 standard distributions below the median is defined as severely stunted.

Weight and length or height will be measured at birth, and 3, 6, 12, 18, and 24 months follow-up. Weight-for-length z-scores (WLZ) will be calculated using the World Health Organization's 2006 Child Growth Standards. WLZ < -2 standard distributions below the median is defined as moderately wasted, and WLZ < -3 standard distributions below the median is defined as severely wasted.

Weight will be measured at birth, and 3, 6, 12, 18, and 24 months follow-up. Weight-for-age z-scores (WAZ) will be calculated using the World Health Organization's 2006 Child Growth Standards. WAZ < -2 standard distributions below the median is defined as moderately underweight, and WAZ < -3 standard distributions below the median is defined as severely underweight.

Percentage of mothers satisfied or very satisfied with their quality of life based on a five-point scale (very satisfied, satisfied, neither satisfied nor unsatisfied, somewhat unsatisfied, unsatisfied)

Mean Perceived Stress Scale score for mothers. The scale ranges from 0-40, and higher scores indicate higher perceived stress. Scores from 0-13 will be classified as mild stress, 14-26 as moderate stress, and 27-40 as severe stress.

Prevalence of maternal depression based on a score of 11 or higher and mean total score using the Edinburgh Postnatal Depression Scale measured at the 6 month follow-up visit. The scale ranges from 0-30, and higher scores indicate presence of more depression symptoms.

Mean maternal Raven's Progressive Matrices score (range: 0-60) and percentile. Higher scores indicate higher non-verbal reasoning.

Mean highest level completed of Corsi Block Span task (range: 1-10). Higher scores indicate higher visual-spatial working memory.

Maternal hours of discretionary time per week measured using hybrid time diaries and activity lists

Prevalence of Ascaris lumbricoides in household floor swabs detected with qPCR in a sub-sample of households at any follow-up measurement (6, 12, 18, 24 months follow-up)

Prevalence of Necator americanus detected in household floor swabs with qPCR in a sub-sample of households at any follow-up measurement (6, 12, 18, 24 months follow-up)

Prevalence of Trichuris trichiura detected in household floor swabs with qPCR in a sub-sample of households at any follow-up measurement (6, 12, 18, 24 months follow-up)

Prevalence of Ascaris lumbricoides, Necator americanus, or Trichuris trichiura detected in household floor swabs with qPCR in a sub-sample of households at any follow-up measurement (6, 12, 18, 24 months follow-up)

Abundance (most probable number per square meter) of E. coli in household floor swabs detected with IDEXX in a sub-sample of households at any follow-up measurement (6, 12, 18, 24 months follow-up)

Abundance (most probable number per two hands) of E. coli on child hands enumerated with IDEXX in a sub-sample of households at any follow-up measurement (6, 12, 18, 24 months follow-up)

Prevalence of other enteric pathogens in household floor samples detected using a TaqMan array card in a sub-sample of households

Mean number of child soil contact events per hour measured using video observations in a sub-sample of the birth cohort at any follow-up measurement (6, 12, 18, 24 months follow-up)

Mean number of child soil ingestion events per hour measured using video observations in a sub-sample of the birth cohort at any follow-up measurement (6, 12, 18, 24 months follow-up)