Back to resultsEffects of Single Plasma Exchange and Double Filtration Plasmapheresis (DFPP) on Peripheral Lymphocyte Phenotypes in Patients With Chronic Inflammatory Demyelinating Polyradiculoneuropathy (LYMPHOARESIS)
Primary Purpose
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating
Status
Completed
Phase
Early Phase 1
Locations
France
Study Type
Interventional
Intervention
Blood sampling
Sponsored by
About this trial
This is an interventional treatment trial for Polyradiculoneuropathy, Chronic Inflammatory Demyelinating focused on measuring Plasma exchange, Double filtration plasmapheresis
Eligibility Criteria
Inclusion Criteria:
- Patients with confirmed Chronic Inflammatory Demyelinating Polyneuropathy (CIDP) without pathogenic antibodies and stable under therapeutic plasmapheresis with one session every 2-3 weeks for at least two months.
- Patients who have given free written informed consent.
- Patients who have signed the consent form.
- Patients affiliated to or beneficiaries of a health insurance scheme.
- Adult patients (≥18 ans).
Exclusion Criteria:
- Patients Under the age of 18.
- Patients under immunosuppressive treatment for less than 3 months.
- Patients participating in Category 1 research involving human subjects.
- Patients in an exclusion period determined by another study.
- Patients under legal protection, guardianship or curatorship.
- Patients unable to express consent.
- Patients for whom it is impossible to give informed information.
- Pregnant, parturient or breastfeeding patients.
Sites / Locations
- Olivier MORANNE
Arms of the Study
Arm 1
Arm 2
Arm Type
Experimental
Experimental
Arm Label
Group A
Group B
Arm Description
Patients alternating single plasma exchange followed by double filtration plasmapheresis etc.
Patients alternating double filtration plasmapheresis followed by single plasma exchange etc.
Outcomes
Primary Outcome Measures
Cycle 1, Group A : TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH17 cells BEFORE first single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH17 cells 7 - 10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : T-reg cells BEFORE first single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 1, Group A : T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 1, Group A : T-reg cells 7 - 10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 1, Group B : TH1 cells BEFORE first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group B : TH1 cells AFTER the first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group B : TH1 cells 7-10 days AFTER the first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group B : TH17 cells BEFORE the first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 1, Group B : TH17 cells AFTER the first double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 1, Group B : T17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 1, Group B : T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 1, Group B : T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 1, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group A: TH1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Cycle 2, Group A: TH1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Cycle 2, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Cycle 2, Group A: TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 2, Group A: TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 2, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 2, Group A: T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group A: T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group B: TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 cells will be measured in %.
Cycle 2, Group B: TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Cycle 2, Group B: TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Cycle 2, Group B: T17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T17 cells will be measured in %.
Cycle 2, Group B: TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T17 cells will be measured in %.
Cycle 2, Group B: TH17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 2, Group B: T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 2, Group B: T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 2, Group B: T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group A: TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group A: TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group A: T1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group A: TH17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 3, Group A: TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 3, Group A: TH17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 3, Group A: T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group A: T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group A: T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group B: TH1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group B: TH1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group B: TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group B: TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 3, Group B: TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 3, Group B: TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 3, Group B: T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 3, Group B: T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 3, Group B: T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 4, Group A: T1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group A: T1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group A: TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 4, Group A: TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 4, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group A: T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 4, Group A: T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 4, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : TH17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 4, Group B : TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 4, Group B : T17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 4, Group B : T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 4, Group B : T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group A : TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group A : TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group A : TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Cycle 5, Group A : TH17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 5, Group A : TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 5, Group A : TH17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 5, Group A : T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group A : T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group A : T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group B : TH1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group B : TH1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group B : TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group B : TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 5, Group B : TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 5, Group B : TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 5, Group B : T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 5, Group B : T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 5, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 6, Group A : TH1 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group A : TH1 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group A : TH1 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group A : TH17 cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 6, Group A : TH17 cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 6, Group A : TH17 cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 6, Group A : T-reg cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 6, Group A : T-reg cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 6, Group A : T-reg cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 6, Group B : TH1 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group B : TH1 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group B : TH1 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group B : TH17 cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 6, Group B : TH17 cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 6, Group B : TH17 cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 6, Group B : T-reg cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 6, Group B : T-reg cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 6, Group B : T-reg cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Secondary Outcome Measures
Cycle 1, Group A : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4-DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4-DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4-DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B:T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B:T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B:T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B:T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured a percentage.
Cycle 2, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CC45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CC45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CC45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 lymphocytes 7 -10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 lymphocytes BEFORE plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 DR+ lymphocytes BEFORE plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 lymphocytes BEFORE plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : B lymphocytes BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : B lymphocytes AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : B lymphocytes BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : B lymphocytes AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Full Information
NCT ID
NCT04742374
First Posted
January 26, 2021
Last Updated
September 25, 2023
Sponsor
Centre Hospitalier Universitaire de Nīmes
1. Study Identification
Unique Protocol Identification Number
NCT04742374
Brief Title
Effects of Single Plasma Exchange and Double Filtration Plasmapheresis (DFPP) on Peripheral Lymphocyte Phenotypes in Patients With Chronic Inflammatory Demyelinating Polyradiculoneuropathy
Acronym
LYMPHOARESIS
Official Title
Comparison of the Effects of 2 Therapeutic Plasmapheresis Techniques: Single Plasma Exchange and Double Filtration Plasmapheresis (DFPP) on Peripheral Lymphocyte Phenotypes in Patients With Chronic Inflammatory Demyelinating Polyradiculoneuropathy (CIDP). A Monocentric Prospective Study With a Single-Case Experimental Design
Study Type
Interventional
2. Study Status
Record Verification Date
July 2022
Overall Recruitment Status
Completed
Study Start Date
February 1, 2021 (Actual)
Primary Completion Date
February 3, 2022 (Actual)
Study Completion Date
February 3, 2022 (Actual)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Sponsor
Name of the Sponsor
Centre Hospitalier Universitaire de Nīmes
4. Oversight
Studies a U.S. FDA-regulated Drug Product
No
Studies a U.S. FDA-regulated Device Product
No
Data Monitoring Committee
No
5. Study Description
Brief Summary
CIDP, a rare disorder affecting young adults, causes gradual weakness of the limbs, areflexia and impaired sensory function. New CIDP phenotypes without antibodies but with modified cell profiles have been described. Treatments include corticotherapy, IVIg and plasmapheresis but the latter's action mechanisms remain unclear. Plasmapheresis supposedly removes toxic agents like antibodies from plasma but it is uncertain whether it has an immune-modulating effect. Also, the refining mechanisms of the two main plasmapheresis techniques - single plasma exchange and double filtration plasmapheresis (DFPP) - are different and unclear. This study aims to compare the evolution of peripheral lymphocyte profiles in patients with CIDP according to their treatment (single centrifugation plasmapheresis or DFPP) to better grasp the action mechanisms of both techniques.
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating
Keywords
Plasma exchange, Double filtration plasmapheresis
7. Study Design
Primary Purpose
Treatment
Study Phase
Early Phase 1
Interventional Study Model
Crossover Assignment
Model Description
Proof-of-concept, monocentric, prospective, Single-Case Experimental Design
Masking
Participant
Masking Description
Randomisation to one of two treatments before alternation.
Allocation
Randomized
Enrollment
8 (Actual)
8. Arms, Groups, and Interventions
Arm Title
Group A
Arm Type
Experimental
Arm Description
Patients alternating single plasma exchange followed by double filtration plasmapheresis etc.
Arm Title
Group B
Arm Type
Experimental
Arm Description
Patients alternating double filtration plasmapheresis followed by single plasma exchange etc.
Intervention Type
Biological
Intervention Name(s)
Blood sampling
Intervention Description
Blood sampling before and after each type of cycle, Single Plasma Exchange or Double Filtration Plasmapheresis
Primary Outcome Measure Information:
Title
Cycle 1, Group A : TH1 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Time 0
Title
Cycle 1, Group A : TH1 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 1, Group A : TH1 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 -10
Title
Cycle 1, Group A : TH17 cells BEFORE first single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Time 0
Title
Cycle 1, Group A : TH17 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 1, Group A : TH17 cells 7 - 10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 1, Group A : T-reg cells BEFORE first single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 1, Group A : T-reg cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 1, Group A : T-reg cells 7 - 10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 1, Group B : TH1 cells BEFORE first double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 1, Group B : TH1 cells AFTER the first double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 1, Group B : TH1 cells 7-10 days AFTER the first double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 1, Group B : TH17 cells BEFORE the first double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day0, Hour 0
Title
Cycle 1, Group B : TH17 cells AFTER the first double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 1, Group B : T17 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 1, Group B : T-reg cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 1, Group B : T-reg cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 1, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A: TH1 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 2, Group A: TH1 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 2, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A: TH17 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 2, Group A: TH17 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 2, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 2, Group A: T-reg cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 2, Group A: T-reg cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 2, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 2, Group B: TH1 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 2, Group B: TH1 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 2, Group B: TH1 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 2, Group B: T17 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T17 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 2, Group B: TH17 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T17 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 2, Group B: TH17 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 2, Group B: T-reg cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 2, Group B: T-reg cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 2, Group B: T-reg cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 3, Group A: TH1 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 1, Hour 1
Title
Cycle 3, Group A: TH1 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 1, Hour 3
Title
Cycle 3, Group A: T1 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 3, Group A: TH17 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 1, Hour 1
Title
Cycle 3, Group A: TH17 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 1, Hour 3
Title
Cycle 3, Group A: TH17 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 3, Group A: T-reg cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 3, Group A: T-reg cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 3, Group A: T-reg cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 3, Group B: TH1 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 3, Group B: TH1 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 3, Group B: TH1 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 3, Group B: TH17 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 3, Group B: TH17 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 3, Group B: TH17 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 3, Group B: T-reg cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 3, Group B: T-reg cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 3, Group B: T-reg cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 4, Group A: T1 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 4, Group A: T1 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 4, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 4, Group A: TH17 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 4, Group A: TH17 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 4, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 4, Group A: T-reg cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 4, Group A: T-reg cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 4, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 4, Group B : TH1 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 4, Group B : TH1 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 4, Group B : TH1 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 -10
Title
Cycle 4, Group B : TH17 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 4, Group B : TH17 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 4, Group B : T17 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7 -10
Title
Cycle 4, Group B : T-reg cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 4, Group B : T-reg cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 4, Group B : T-reg cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 5, Group A : TH1 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : TH1 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : TH1 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 5, Group A : TH17 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : TH17 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : TH17 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 5, Group A : T-reg cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : T-reg cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : T-reg cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 7 - 10
Title
Cycle 5, Group B : TH1 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : TH1 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : TH1 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 5, Group B : TH17 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : TH17 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : TH17 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 5, Group B : T-reg cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : T-reg cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 6, Group A : TH1 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0 Hour 0
Title
Cycle 6, Group A : TH1 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0 Hour 3
Title
Cycle 6, Group A : TH1 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 6, Group A : TH17 cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0 Hour 0
Title
Cycle 6, Group A : TH17 cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 0 Hour 3
Title
Cycle 6, Group A : TH17 cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 6, Group A : T-reg cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0 Hour 0
Title
Cycle 6, Group A : T-reg cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 0 Hour 3
Title
Cycle 6, Group A : T-reg cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 6, Group B : TH1 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0 Hour 0
Title
Cycle 6, Group B : TH1 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 0 Hour 3
Title
Cycle 6, Group B : TH1 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 6, Group B : TH17 cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 0 Hour 0
Title
Cycle 6, Group B : TH17 cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 0 Hour 3
Title
Cycle 6, Group B : TH17 cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Time Frame
Day 7-10
Title
Cycle 6, Group B : T-reg cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0 Hour 0
Title
Cycle 6, Group B : T-reg cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 0 Hour 3
Title
Cycle 6, Group B : T-reg cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Time Frame
Day 7-10
Secondary Outcome Measure Information:
Title
Cycle 1, Group A : T4 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : T4-DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : T8 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : B lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group A : T4 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : T4-DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : T8 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : B lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : CR6-CXCR3- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group A : T4-DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: T4 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B: T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B: T8 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B: T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B: B lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B: CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B: CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B: CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B: CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 1, Group B:T4 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B:T4 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B:T8 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B:T8 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B: B lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B: CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B: CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B: CD45RA-CCR7- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B: CCR6-CXCR3- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 1, Group B: T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: B lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 1, Group B: CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : B lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : B lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7 - 10
Title
Cycle 2, Group B : T4 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : T8 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : B lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 2, Group B : T4 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : T8 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : B lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 2, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured a percentage.
Time Frame
Day 7-10
Title
Cycle 2, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : T4 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : T8 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : B lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : CC45RA+CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : CC45RA-CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : CC45RA-CCR7- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group A : T4 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : T4 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : T8 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : B lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : CCR6-CXCR3- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : T4 lymphocytes 7 -10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : B lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 3, Group B : T4 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : T8 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : B lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 3, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 3, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : B lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : B lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : T4 lymphocytes BEFORE plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : T4 DR+ lymphocytes BEFORE plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : T8 lymphocytes BEFORE plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : B lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 0
Title
Cycle 4, Group B : T4 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : T8 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : B lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0 Hour 3
Title
Cycle 4, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 4, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : T4 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : T8 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : B lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group A : T4 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : T4 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : T8 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : T8 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : B lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : CD45RA-CCR7- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : CCR6-CXCR3- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : B lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : B lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 5, Group B : T4 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : T8 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : B lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 5, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 5, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : B lymphocytes BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 0
Title
Cycle 6, Group A : T4 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : T8 lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : B lymphocytes AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, Hour 3
Title
Cycle 6, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis
Description
5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : T4 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : T8 lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : B lymphocytes BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H0
Title
Cycle 6, Group B : T4 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : T4 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : T8 lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : T8 DR+ lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : B lymphocytes AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : CD45RA-CCR7- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : CCR6-CXCR3- cells AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 0, H3
Title
Cycle 6, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : B lymphocytes 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Title
Cycle 6, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange
Description
5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Time Frame
Day 7-10
Other Pre-specified Outcome Measures:
Title
Group A, Cycle 1 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours)
Title
Group B, Cycle 1 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours)
Title
Group A, Cycle 2 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours)
Title
Group B, Cycle 2 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours)
Title
Group A, Cycle 3 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours)
Title
Group B, Cycle 3 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours)
Title
Group A, Cycle 4 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours)
Title
Group B, Cycle 4 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours)
Title
Group A, Cycle 5 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours)
Title
Group B, Cycle 5 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours)
Title
Group A, Cycle 6 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours)
Title
Group B, Cycle 6 : Link between variations in lymphocyte sub-populations and the clinical evolution .
Description
All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.
Time Frame
Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours)
Title
Group A, Cycle 1 : Cost of single plasma exchange to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours)
Title
Group B, Cycle 1 : Cost of double filtration plasmapheresis to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours)
Title
Group A, Cycle 2 : Cost of double filtration plasmapheresis to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours)
Title
Group B, Cycle 2 : Cost of single plasma exchange to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours)
Title
Group A, Cycle 3 : Cost of single plasma exchange to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours)
Title
Group B, Cycle 3 : Cost of double filtration plasmapheresis to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours)
Title
Group A, Cycle 4 : Cost of double filtration plasmapheresis to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours)
Title
Group B, Cycle 4 : Cost of single plasma exchange to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours)
Title
Group A, Cycle 5 : Cost of single plasma exchange to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours)
Title
Group B, Cycle 5 : Cost of double filtration to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours)
Title
Group A, Cycle 6 : Cost of double filtration to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours)
Title
Group B, Cycle 6 : Cost of double filtration to the health institution.
Description
The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.
Time Frame
Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours)
Title
Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping.
Description
After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed.
Time Frame
Day 0, Hour 0
Title
Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping.
Description
After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed.
Time Frame
Day 0, Hour 3
Title
Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping.
Description
After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed.
Time Frame
Day 7-10
Title
Compare lymphocyte phenotype between patients with PIDC and control subjects.
Description
Significant difference in Th1/Th2 ratio between PIDC patients and control subjects.
Time Frame
Day 0
10. Eligibility
Sex
All
Minimum Age & Unit of Time
18 Years
Accepts Healthy Volunteers
No
Eligibility Criteria
Inclusion Criteria:
Patients with confirmed Chronic Inflammatory Demyelinating Polyneuropathy (CIDP) without pathogenic antibodies and stable under therapeutic plasmapheresis with one session every 2-3 weeks for at least two months.
Patients who have given free written informed consent.
Patients who have signed the consent form.
Patients affiliated to or beneficiaries of a health insurance scheme.
Adult patients (≥18 ans).
Healthy subjects of the same age and sex
Exclusion Criteria:
Patients Under the age of 18.
Patients under immunosuppressive treatment for less than 3 months.
Patients participating in Category 1 research involving human subjects.
Patients in an exclusion period determined by another study.
Patients under legal protection, guardianship or curatorship.
Patients unable to express consent.
Patients for whom it is impossible to give informed information.
Pregnant, parturient or breastfeeding patients.
Healthy subject with chronic pathology or drug treatment
Facility Information:
Facility Name
Olivier MORANNE
City
Nîmes
Country
France
12. IPD Sharing Statement
Citations:
PubMed Identifier
35931957
Citation
Moranne O, Ion IM, Cezar R, Messikh Z, Prelipcean C, Chkair S, Thouvenot E, Tran TA, Corbeau P, Chevallier T. Protocol of comparison of the effects of single plasma exchange and double filtration plasmapheresis on peripheral lymphocyte phenotypes in patients with Chronic Inflammatory Demyelinating Polyradiculoneuropathy: a monocentric prospective study with single-case experimental design. BMC Neurol. 2022 Aug 5;22(1):293. doi: 10.1186/s12883-022-02816-w.
Results Reference
derived
Learn more about this trial
Effects of Single Plasma Exchange and Double Filtration Plasmapheresis (DFPP) on Peripheral Lymphocyte Phenotypes in Patients With Chronic Inflammatory Demyelinating Polyradiculoneuropathy
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