FISH in Diagnosis of Biliary Stricture
Biliary Stricture
About this trial
This is an interventional diagnostic trial for Biliary Stricture focused on measuring suspected malignant biliary stricture, fluorescence in situ hybridization, endoscopic retrograde cholangiopancreatography, brushing, forceps biopsy, endosonography/ EUS-FNA
Eligibility Criteria
Inclusion Criteria:
- Suspected malignant biliary stricture
- Localization: Extrahepatic biliary duct
- Patient´s consent with a diagnostic procedure
- Age : 18 years or more
Exclusion Criteria:
- Intrahepatic biliary strictures
- Duodenal stenosis (endoscopically)
- Age : < 18 years
- Coagulopathy : (INR >1,5, Platelets < 100)
- Pregnancy
Sites / Locations
- 2nd Department of Internal Medicine, University Hospital and Palacký University,Recruiting
Arms of the Study
Arm 1
Arm 2
Active Comparator
Active Comparator
conventional samplig
Fluorescence in situ Hybridization (FISH)
Patients with biliary strictures udergo ERCP or EUS. Tissue specimens obtained via either of brush cytology, forceps biopsy or fine needle aspiration during ERCP or endosonography (EUS) were examined by routine cytology or histology methods. Gold standard for final diagnosis is the histology from surgical resection. In patients without surgery, a follow up of 12 months will be considered adequate to exclude or confirm malignant etiology.
Tissue specimens obtained via either of brush cytology, forceps biopsy or fine needle aspiration during ERCP or endosonography (EUS) were examined by routine cytology or histology methods. In addition, FISH inlcuding fluorescence-based polynucleotide probes targeting chromosomes 3, 7, 17 and locus 9p21 was performed. Gold standard for final diagnosis is the histology from surgical resection. In patients without surgery, a follow up of 12 months will be considered adequate to exclude or confirm malignant etiology.