Food First Approach to Stimulate Muscle Protein Synthesis in Healthy Adults (Salmon)
Primary Purpose
Hypertrophy
Status
Completed
Phase
Not Applicable
Locations
United States
Study Type
Interventional
Intervention
Resistance exercise
Salmon
Isolated amino acids and fatty acids
Sponsored by
About this trial
This is an interventional basic science trial for Hypertrophy
Eligibility Criteria
Inclusion Criteria:
- Age 20-35 years
- Recreationally-active adults: ≥ 30 min of physical activity at moderate intensity ≥ 3 times per week
- English fluency
Exclusion Criteria:
- Smoking
- Known allergies to fish consumption
- Vegans
- Diagnosed GI tract diseases
- Arthritic conditions
- A history of neuromuscular problems
- Diagnosed cognitive impairments
- Recent (1 year) participation in amino acid tracer studies
- Predisposition to hypertrophic scarring or keloid formation
- Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
- High blood pressure (Systolic > 140 mm HG; Diastolic > 90 mm HG)
- Alcohol consumption >10 drinks per week
- Metabolic disorders (e.g., Metabolic Syndrome, diabetes, thyroid diseases)
- Consumption of thyroid, androgenic or other medications known to affect endocrine function
- Consumption of ergogenic-leves of dietary supplements that may affect muscle mass (e.g., creatine, HMB), insulin-like substances, or anabolic/catabolic pro-hormones (e.g., DHEA) within 6 weeks prior to participation
- Currently pregnant
- A suboptimal diet quality score as assessed by ASA24 dietary records
- Asthma
- Hypogonadism
- Cardiovascular disease, arrhythmias
Sites / Locations
- Freer Hall
Arms of the Study
Arm 1
Arm 2
Arm Type
Active Comparator
Experimental
Arm Label
Salmon
Isolated mixture
Arm Description
After resistance exercise, participants will ingest 3.5 oz of salmon fillet (21g protein, 24g fat) cooked sous-vide.
After resistance exercise, participants will ingest an isolated amino acid and fatty acid mixture matched to the amino acid and fatty acid content of 3.5 oz salmon fillet.
Outcomes
Primary Outcome Measures
Fractional synthetic rate of myofibrillar proteins
Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hr and during the 5 hr after the ingestion of the experimental interventions. This will allow us to assess the change from the postabsorptive to the postprandial period.
Secondary Outcome Measures
Phosphorylation of muscle anabolic signaling
Phosphorylation of anabolic signaling pathways will be assessed in the fasted state and at 2 and 5 hr after the ingestion of the experimental interventions.
Exogenous rate of leucine appearance
Leucine oxidation rates will be assessed in the fasted state and at multiple time points in the postprandial phase.
Full Information
NCT ID
NCT03870165
First Posted
March 8, 2019
Last Updated
September 24, 2020
Sponsor
University of Illinois at Urbana-Champaign
1. Study Identification
Unique Protocol Identification Number
NCT03870165
Brief Title
Food First Approach to Stimulate Muscle Protein Synthesis in Healthy Adults
Acronym
Salmon
Official Title
Food First Approach to Stimulate Muscle Protein Synthesis in Healthy Adults
Study Type
Interventional
2. Study Status
Record Verification Date
September 2020
Overall Recruitment Status
Completed
Study Start Date
March 1, 2019 (Actual)
Primary Completion Date
November 22, 2019 (Actual)
Study Completion Date
November 22, 2019 (Actual)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
University of Illinois at Urbana-Champaign
4. Oversight
Studies a U.S. FDA-regulated Drug Product
No
Studies a U.S. FDA-regulated Device Product
No
Data Monitoring Committee
No
5. Study Description
Brief Summary
In a crossover design 10 young healthy adults (20-35 y) will receive stable isotope tracer infusions and perform a single bout of resistance exercise. Immediately after exercise participants will ingest either 3.5 oz of Salmon fillet or its constituent macronutrients as isolated amino acids and fat. Repeated blood and breath samples as well as muscle biopsies will be collected to determine whole body amino acid kinetics, muscle amino acid transporters, anabolic signalling and myofibrillar protein synthesis rates during the trials
Detailed Description
On both infusion trials, participants will report to the laboratory at 0700 h after an overnight fast. Upon arrival to the lab, the participant will fill out a questionnaire and a baseline breath sample will be collected to determine 13CO2 enrichment by isotope ratio mass spectrometry. A Teflon catheter will be inserted into the antecubital vein for a baseline blood sample (t=-210) and then participants will receive priming doses of NaH13CO2 (2.35 µmol·kg-1), L-[1-13C]leucine (7.6 µmol·kg-1), and L-[ring-2H5]phenylalanine (2.0 µmol·kg-1). Subsequently, a continuous intravenous solution of L-[1-13C]leucine (0.10 µmol·kg-1·min-1) and L-[ring-2H5]phenylalanine (0.05 µmol·kg-1·min-1) will be initiated (t=-210) and maintained over the infusion trials. A second Teflon catheter will be inserted into a heated dorsal vein for repeated arterialized blood sampling and remained patent by a 0.9% saline drip. Breath samples and arterialized blood samples will be collected every 30 to 60 minutes during the postabsorptive and postprandial states. In the post-absorptive state of infusion trial 1, muscle biopsies will be collected at t=-150 and -30 min of infusion to determine basal-state myofibrillar protein synthesis rates, relative skeletal muscle amino acid transporter content, and anabolic-related signaling. In the subsequent cross-over trial only 1 muscle biopsy will be collected at t=-30 for Western blot analysis and postabsorptive myofibrillar protein-bound tracer enrichment. After collection of the resting muscle biopsy at t=-30 for both trials, the participants will perform resistance exercise that consists of 4 sets of 10-12 repetitions or to muscular failure at 65-70% of 1-RM for both leg press and leg extension exercise.
Immediately after the completion of the exercise bout, participants will ingest either 3.5 oz of Salmon fillet or a matched isolated amino acid and fat mixture (t=0). Completion of the meal will mark the start of the postprandial stage and additional muscle biopsies will be collected at t=120 and t=300.
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Hypertrophy
7. Study Design
Primary Purpose
Basic Science
Study Phase
Not Applicable
Interventional Study Model
Crossover Assignment
Masking
None (Open Label)
Allocation
Randomized
Enrollment
10 (Actual)
8. Arms, Groups, and Interventions
Arm Title
Salmon
Arm Type
Active Comparator
Arm Description
After resistance exercise, participants will ingest 3.5 oz of salmon fillet (21g protein, 24g fat) cooked sous-vide.
Arm Title
Isolated mixture
Arm Type
Experimental
Arm Description
After resistance exercise, participants will ingest an isolated amino acid and fatty acid mixture matched to the amino acid and fatty acid content of 3.5 oz salmon fillet.
Intervention Type
Behavioral
Intervention Name(s)
Resistance exercise
Intervention Description
Participants will perform leg press and leg extension immediately prior to ingestion of salmon or isolated mixture
Intervention Type
Other
Intervention Name(s)
Salmon
Intervention Description
Participants will ingest salmon immediately after resistance exercise
Intervention Type
Dietary Supplement
Intervention Name(s)
Isolated amino acids and fatty acids
Intervention Description
Participants will ingest a mixture of isolated amino acids and fatty acids immediately after resistance exercise
Primary Outcome Measure Information:
Title
Fractional synthetic rate of myofibrillar proteins
Description
Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hr and during the 5 hr after the ingestion of the experimental interventions. This will allow us to assess the change from the postabsorptive to the postprandial period.
Time Frame
Postabsorptive for 3 hours, postprandial for 5 hours.
Secondary Outcome Measure Information:
Title
Phosphorylation of muscle anabolic signaling
Description
Phosphorylation of anabolic signaling pathways will be assessed in the fasted state and at 2 and 5 hr after the ingestion of the experimental interventions.
Time Frame
Baseline and at 2 and 5 hours after protein ingestion
Title
Exogenous rate of leucine appearance
Description
Leucine oxidation rates will be assessed in the fasted state and at multiple time points in the postprandial phase.
Time Frame
5 hour postprandial phase
10. Eligibility
Sex
All
Minimum Age & Unit of Time
20 Years
Maximum Age & Unit of Time
35 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria:
Age 20-35 years
Recreationally-active adults: ≥ 30 min of physical activity at moderate intensity ≥ 3 times per week
English fluency
Exclusion Criteria:
Smoking
Known allergies to fish consumption
Vegans
Diagnosed GI tract diseases
Arthritic conditions
A history of neuromuscular problems
Diagnosed cognitive impairments
Recent (1 year) participation in amino acid tracer studies
Predisposition to hypertrophic scarring or keloid formation
Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
High blood pressure (Systolic > 140 mm HG; Diastolic > 90 mm HG)
Alcohol consumption >10 drinks per week
Metabolic disorders (e.g., Metabolic Syndrome, diabetes, thyroid diseases)
Consumption of thyroid, androgenic or other medications known to affect endocrine function
Consumption of ergogenic-leves of dietary supplements that may affect muscle mass (e.g., creatine, HMB), insulin-like substances, or anabolic/catabolic pro-hormones (e.g., DHEA) within 6 weeks prior to participation
Currently pregnant
A suboptimal diet quality score as assessed by ASA24 dietary records
Asthma
Hypogonadism
Cardiovascular disease, arrhythmias
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Nicholas A Burd, PhD
Organizational Affiliation
University of Illinois at Urbana-Champaign
Official's Role
Principal Investigator
Facility Information:
Facility Name
Freer Hall
City
Urbana
State/Province
Illinois
ZIP/Postal Code
61801
Country
United States
12. IPD Sharing Statement
Plan to Share IPD
No
Learn more about this trial
Food First Approach to Stimulate Muscle Protein Synthesis in Healthy Adults
We'll reach out to this number within 24 hrs