Genetic Analysis in Diagnosing Thyroid Cancer in Patients With Thyroid Nodules
Primary Purpose
Head and Neck Cancer
Status
Completed
Phase
Not Applicable
Locations
United States
Study Type
Interventional
Intervention
gene expression analysis
polymerase chain reaction
reverse transcriptase-polymerase chain reaction
diagnostic laboratory biomarker analysis
immunohistochemistry staining method
needle biopsy
Sponsored by
About this trial
This is an interventional diagnostic trial for Head and Neck Cancer focused on measuring thyroid cancer
Eligibility Criteria
DISEASE CHARACTERISTICS: Diagnosis of a thyroid nodule that requires fine-needle aspiration biopsy or thyroidectomy to exclude a diagnosis of thyroid cancer PATIENT CHARACTERISTICS: Platelet count ≥ 70,000/mm^3 PT (INR) ≤ 14.5 PTT ≤ 35.2 Not pregnant PRIOR CONCURRENT THERAPY: No aspirin or nonsteroidal anti-inflammatory drugs (NSAIDs) 7 days before sample procurement
Sites / Locations
- UCSF Helen Diller Family Comprehensive Cancer Center
Outcomes
Primary Outcome Measures
Diagnostic accuracy of biomarkers by mRNA expression analysis
Correlate levels of gene expression with the aggressiveness of differentiated thyroid cancer in FNA biopsy samples
Secondary Outcome Measures
Full Information
NCT ID
NCT00316823
First Posted
April 19, 2006
Last Updated
February 21, 2014
Sponsor
University of California, San Francisco
Collaborators
National Cancer Institute (NCI)
1. Study Identification
Unique Protocol Identification Number
NCT00316823
Brief Title
Genetic Analysis in Diagnosing Thyroid Cancer in Patients With Thyroid Nodules
Official Title
Gene Expression Analysis in Thyroid Nodule FNA Samples
Study Type
Interventional
2. Study Status
Record Verification Date
December 2008
Overall Recruitment Status
Completed
Study Start Date
July 2005 (undefined)
Primary Completion Date
December 2009 (undefined)
Study Completion Date
undefined (undefined)
3. Sponsor/Collaborators
Name of the Sponsor
University of California, San Francisco
Collaborators
National Cancer Institute (NCI)
4. Oversight
5. Study Description
Brief Summary
RATIONALE: Finding genetic markers for thyroid cancer in a biopsy specimen may help doctors diagnose thyroid cancer.
PURPOSE: This clinical trial is studying how well genetic analysis works in diagnosing thyroid cancer in patients with thyroid nodules.
Detailed Description
OBJECTIVES:
Evaluate the diagnostic accuracy of biomarkers and mRNA expression analysis using fine-needle aspiration (FNA) biopsy samples from patients with thyroid nodules.
Correlate the level of gene expression with the aggressiveness of differentiated thyroid cancer in FNA biopsy samples.
OUTLINE: Patients undergo fine-needle aspiration (FNA) biopsy as part of routine care. Additional thyroid tissue is collected during the biopsy for gene expression analysis. Patients who have already had a biopsy undergo FNA biopsy during thyroidectomy.
Tissue samples will be examined for gene expression by reverse transcriptase-polymerase chain reaction (PCR), real-time quantitative PCR, and immunohistochemistry.
PROJECTED ACCRUAL: A total of 400 patients will be accrued for this study.
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Head and Neck Cancer
Keywords
thyroid cancer
7. Study Design
Primary Purpose
Diagnostic
Study Phase
Not Applicable
Masking
None (Open Label)
Enrollment
400 (Anticipated)
8. Arms, Groups, and Interventions
Intervention Type
Genetic
Intervention Name(s)
gene expression analysis
Intervention Type
Genetic
Intervention Name(s)
polymerase chain reaction
Intervention Type
Genetic
Intervention Name(s)
reverse transcriptase-polymerase chain reaction
Intervention Type
Other
Intervention Name(s)
diagnostic laboratory biomarker analysis
Intervention Type
Other
Intervention Name(s)
immunohistochemistry staining method
Intervention Type
Procedure
Intervention Name(s)
needle biopsy
Primary Outcome Measure Information:
Title
Diagnostic accuracy of biomarkers by mRNA expression analysis
Title
Correlate levels of gene expression with the aggressiveness of differentiated thyroid cancer in FNA biopsy samples
10. Eligibility
Sex
All
Minimum Age & Unit of Time
18 Years
Accepts Healthy Volunteers
No
Eligibility Criteria
DISEASE CHARACTERISTICS:
Diagnosis of a thyroid nodule that requires fine-needle aspiration biopsy or thyroidectomy to exclude a diagnosis of thyroid cancer
PATIENT CHARACTERISTICS:
Platelet count ≥ 70,000/mm^3
PT (INR) ≤ 14.5
PTT ≤ 35.2
Not pregnant
PRIOR CONCURRENT THERAPY:
No aspirin or nonsteroidal anti-inflammatory drugs (NSAIDs) 7 days before sample procurement
Overall Study Officials:
First Name & Middle Initial & Last Name & Degree
Electron Kebebew, MD
Organizational Affiliation
University of California, San Francisco
Official's Role
Principal Investigator
Facility Information:
Facility Name
UCSF Helen Diller Family Comprehensive Cancer Center
City
San Francisco
State/Province
California
ZIP/Postal Code
94115
Country
United States
12. IPD Sharing Statement
Learn more about this trial
Genetic Analysis in Diagnosing Thyroid Cancer in Patients With Thyroid Nodules
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