Hydroponic Fortification and Dietary App Effect on Nutrients Level (Harvest)

Inclusion Criteria: Women over 18 years with a regular menstruation cycle (>21 and <40 days). Has followed a vegan or vegetarian diet that does not include the consumption of meat or fish for at least 1 year. Willing to remain on their background diet for the duration of the study. Those who are on a routine regimen of iron and/or vitamin B12 supplements must maintain the same doses 3 month prior to the study and during the duration of the study. Non-anaemic, haemoglobin (Hb) concentration ≥120 g/L. SF >15 µg/L and <40 µg/L [WHO uses the following cut-offs for context: below 15 µg/L indicates iron deficiency, 16-40 µg/L indicates borderline deficiency, and 41 - 150 µg/L is normal]. Body Mass Index (BMI) between 18.5 - 40 kg/m2. Live within 40 miles of the Norwich Research Park. Willing to consume plants grown in the hydroponic unit (e.g., spinach, rocket, mizuna) if they agreed to have a unit. Access to a smartphone or online platform a well as access to the internet. Exclusion Criteria: Those who have known allergies to the hydroponically grown salad greens (spinach, rocket, mizuna). Flexitarian (consumes meat intermittently) vegetarian. Those with menstruation cycle time periods of <21 or >40-days BMI at screening visit < 18.5 kg/m2 (underweight) and > 40 kg/m2 (severely obese). Those diagnosed with or undergoing treatment for anaemia. Currently pregnant, lactating or trying to conceive. High alcohol consumption, consuming more than 2 drinks per day, can elevate the risk for iron overload. Current smoker or has only ceased in the last 6 months. Diagnosed medical conditions that may impact the study outcomes will be considered on an individual basis. Those with an eating disorders, which may impact the study outcome. On, or about to start, a diet programme such as the 5:2 programme (5:2 programme is where an individual would eat normally for five days a week, and fast for two days a week by reducing calorie consumption). Unable to give written or verbal informed consent. Individuals who cannot understand the written instructions/questionnaires. Unwilling to give GP contact details. Participating in another dietary intervention study or has given blood in another research study during the last 3 months. Any person related to or living with any member of the study team. Those who are part of the Line Manager/supervisory structure of the Chief Investigator. Those who have been asked to self-isolate or have been diagnosed with COVID-19 in the last 14 days. However, participants can be rescreened 7 days after the isolation period.