Reproductive Outcome After Sperm Selection for Intracytoplasmic Sperm Injection (ICSI) Using Zona Pellucida Proteins
Infertility, Male
About this trial
This is an interventional treatment trial for Infertility, Male focused on measuring zona pellucida, sperm selection, ICSI, ART outcomes
Eligibility Criteria
Inclusion Criteria:
- Clinical diagnosis for primary or secondary infertility
- Healthy females
- Clinical diagnosis for unexplained infertility
- Females between 20 and 45 years old.
- Males between 20 and 50 years old.
- At least 3 retrieved oocytes per follicular puncture with at least 1 oocyte at germinal vesicle (GV) or metaphase I (MI) stage
Exclusion Criteria:
- Presence of low quality oocytes
- Females with endometrial or ovarian infertility factor
Sites / Locations
- Nadezhda Women's Health Hospital
Arms of the Study
Arm 1
Arm 2
Experimental
Active Comparator
Zona pellucida sperm selection
Conventional sperm selection
Semen processing: liquefaction followed by centrifugation and swim-up. Zona pellucida sperm selection: Spermatozoa are selected according to their ability to adhere to zona pellucida proteins obtained from their partners native zona pellucida. Intracytoplasmic sperm injection (ICSI): Each oocyte is injected with a single morphologically normal spermatozoa. The injection procedure is carried out in a sterilized dish using holding pipette and injection needle.
Semen processing: liquefaction followed by centrifugation and swim-up. Samples are incubated until time of injection. Conventional sperm selection: Spermatozoa are selected according to their morphology. Intracytoplasmic sperm injection (ICSI): Each oocyte is injected with a single morphologically normal spermatozoa. The injection procedure is carried out in a sterilized dish using holding pipette and injection needle.