Rewilding the Human Gut: Reintroduction of the Species Limosilactobacillus Reuteri
Microbial Colonization
About this trial
This is an interventional other trial for Microbial Colonization focused on measuring Gut microbiome
Eligibility Criteria
Inclusion Criteria:
- Healthy individuals with a body mass index between 20-29.9 kg/m²
- Have at least one bowel movement per day
- Willing to consume prepared study foods (breakfast, lunch dinner, snacks) for a period of 3 weeks
- Men and pre-menopausal, non-pregnant or non-lactating women
- Non-vegetarian, non-smoking, and alcohol intake ≤8 drinks/week, and willing to consume 8 drinks per week or less during the course of the study.
If consuming probiotic containing foods, willing to discontinue eating same, and substitute with non-probiotic containing foods
-≤5 h/week of moderate-vigorous exercise.
- Quantity of L. reuteri in screening fecal sample below 10^4 CFU/g
Exclusion Criteria:
- History of diabetes, acute or chronic GI illnesses, conditions, or history of GI surgical intervention
- antibiotic treatment in the last 3 months
- use of dietary supplements (including prebiotics and probiotics, fiber supplements/bars, digestive enzymes/beano)- if consumed, willing to undergo 4 week pre-intervention washout period, and remain free of supplements for duration of study. Exception: multivitamin or vitamin d supplement (1 week washout period)
- use of antihypertensive, lipid-lowering, anti-diabetic, anti-inflammatory (i.e corticosteroids or chronic NSAID use), or laxative medications
- known food allergies or intolerances (including dairy allergic or lactose intolerant)
Sites / Locations
- University of Alberta
Arms of the Study
Arm 1
Arm 2
Arm 3
Arm 4
Arm 5
Arm 6
Experimental
Experimental
Placebo Comparator
Experimental
Experimental
Placebo Comparator
L. reuteri PB-W1, Non-Industrialized-Type Diet Start
L. reuteri DSM20016T, Non-Industrialized-Type Diet Start
Placebo, Non-Industrialized-Type Diet Start
L. reuteri PB-W1, Usual Diet Start
L. reuteri DSM20016T, Usual Diet Start
Placebo, Usual Diet Start
Participants will receive the non-industrialized-type diet for 3 weeks, followed by a crossover to 3 weeks of consuming their usual diet after a 3-week washout period. Participants will be provided with a one-time dose of L. reuteri PB-W1 strain on day 4 of each diet period. The L. reuteri PB-W1 strain will be provided as a drinkable solution (approximately 2.25x10^10 viable cells will be provided in 50 ml of water).
Participants will receive the non-industrialized-type diet for 3 weeks, followed by a crossover to 3 weeks of consuming their usual diet after a 3-week washout period. Participants will be provided with a one-time dose of L. reuteri DSM20016T strain on day 4 of each diet period. The L. reuteri DSM20016T strain will be provided as a drinkable solution (approximately 2.25x10^10 viable cells will be provided in 50 ml of water).
Participants will receive the non-industrialized-type diet for 3 weeks, followed by a crossover to 3 weeks of consuming their usual diet after a 3-week washout period. Participants will be provided with a one-time dose of a placebo solution on day 4 of each diet period. The placebo solution will be provided as a drinkable solution (2 g maltodextrin dissolved in 50 ml water in food grade conditions).
Participants will consume their usual diet for 3 weeks, followed by a crossover to 3 weeks of consuming the provided non-industrialized-type diet after a 3-week washout period. Participants will be provided with a one-time dose of L. reuteri PB-W1 strain on day 4 of each diet period. The L. reuteri PB-W1 strain will be provided as a drinkable solution (approximately 2.25x10^10 viable cells will be provided in 50 ml of water).
Participants will consume their usual diet for 3 weeks, followed by a crossover to 3 weeks of consuming the provided non-industrialized-type diet after a 3-week washout period. Participants will be provided with a one-time dose of L. reuteri DSM20016T strain on day 4 of each diet period. The L. reuteri DSM20016T strain will be provided as a drinkable solution (approximately 2.25x10^10 viable cells will be provided in 50 ml of water).
Participants will consume their usual diet for 3 weeks, followed by a crossover to 3 weeks of consuming the provided non-industrialized-type diet after a 3-week washout period. Participants will be provided with a one-time dose of a placebo solution on day 4 of each diet period. The placebo solution will be provided as a drinkable solution (2 g maltodextrin dissolved in 50 ml water in food grade conditions).