Sex Differences in Myocardial Steatosis Induced Left Ventricular Dysfunction
Heart Diseases, Left Ventricular Dysfunction
About this trial
This is an interventional basic science trial for Heart Diseases focused on measuring myocardial steatosis, fasting, estrogen, biological sex as a variable
Eligibility Criteria
Inclusion Criteria:
- blood pressure <140/90 mmHg
- BMI between 18.5 and 30 kg/m2
- sedentary or recreationally active (<3 days of vigorous aerobic exercise each week)
- no use of oral contraceptives hormone therapy, or other medications that might influence cardiovascular function
- nonsmokers.
Exclusion Criteria:
Women will be excluded with
- history of or active estrogen-dependent neoplasms, acute liver or gallbladder disease, vaginal bleeding, venous thromboembolism, hypertriglyceridemia, and CVD
- known allergy to transdermal patch, GnRHant (i.e., hypersensitivity to cetrorelix, extrinsic peptide hormones, mannitol, GnRH, benzyl alcohol - the vehicle for injection of cetrorelix)
- history of stomach ulcer or bleeding
- other contraindications to hormone replacement therapy or GnRHant, (i.e., taking Levodopa, medications that can inhibit folate metabolism including methotrexate).
Other conditions for which individuals will be excluded from the study include:
diabetes active infection history of seizures or disease that affects the nervous system sepsis an abnormal resting ECG contraindications to MRI pregnant or planning to become pregnant smoking history illicit drug use (excluding occasional marijuana).
Sites / Locations
- University of Texas at ArlingtonRecruiting
Arms of the Study
Arm 1
Arm 2
Arm 3
Arm 4
Experimental
Experimental
Experimental
Experimental
Fasting only
LBNP Only
Estrogen add back with GnRHant
Placebo add back with GnRHant
To acutely elevate myocardial triglyceride content, subjects will be asked to abstain from eating for 2 days (reproducibly causes a significant and physiological increase in myocardial fat deposition, transiently). Subjects will be allowed water and/or an isotonic saline solution in order to maintain hydration status.
Subjects undergo lower body negative pressure at 40 mmHg.
Subjects are given estradiol patch.
Subjects are given placebo patch.