The Effect of Water Intake on Glucose Regulation (INDIGO-I)
Primary Purpose
Glucose Metabolism Disorders, Dehydration (Physiology), Water Deprivation
Status
Withdrawn
Phase
Not Applicable
Locations
Study Type
Interventional
Intervention
Water intake manipulation
Sponsored by
About this trial
This is an interventional basic science trial for Glucose Metabolism Disorders focused on measuring water, vasopressin
Eligibility Criteria
Inclusion Criteria:
BMI 27.5-35
Exclusion Criteria:
- BMI <27.5 or >35
- high physical activity
- diabetes
- impaired liver or kidney function
- cardiovascular disease
- weight change of more than 3 kg in the past month
- pregnancy
- previous surgery on digestive tract (except appendectomy)
- fluid balance will be exclusionary such as
- serotonin re-uptake inhibitors.
Sites / Locations
Arms of the Study
Arm 1
Arm 2
Arm Type
Active Comparator
Experimental
Arm Label
High Water intake
Low Water Intake
Arm Description
5-days of High water intake according to IOM guidelines
5-days of Low water intake of 500 mL/day
Outcomes
Primary Outcome Measures
amount of glucose metabolized M
amount of glucose metabolized during the euglycemic & Hyperglycemic clamp
metabolic clearance rate for insulin
metabolic clearance rate for insulin during the euglycemic & Hyperglycemic clamp
quantity of glucose metabolized per unit plasma insulin concentration
quantity of glucose metabolized per unit plasma insulin concentration during the euglycemic & Hyperglycemic clamp
Rate of glucose appearance & disappearance
Rate of glucose appearance & disappearance during the euglycemic & Hyperglycemic clamp
Secondary Outcome Measures
Plasma copeptin
Plasma copeptin in response to water manipulation
glucagon
glucagon in response to water manipulation
Cortisol
Cortisol in response to water manipulation
Full Information
NCT ID
NCT03765008
First Posted
December 3, 2018
Last Updated
August 23, 2019
Sponsor
Arizona State University
1. Study Identification
Unique Protocol Identification Number
NCT03765008
Brief Title
The Effect of Water Intake on Glucose Regulation
Acronym
INDIGO-I
Official Title
The Effect of Water Intake on Glucose Regulation: A Pilot Study
Study Type
Interventional
2. Study Status
Record Verification Date
August 2019
Overall Recruitment Status
Withdrawn
Why Stopped
Study did not get funded
Study Start Date
February 11, 2019 (Anticipated)
Primary Completion Date
April 3, 2019 (Anticipated)
Study Completion Date
April 29, 2019 (Anticipated)
3. Sponsor/Collaborators
Responsible Party, by Official Title
Principal Investigator
Name of the Sponsor
Arizona State University
4. Oversight
Studies a U.S. FDA-regulated Drug Product
No
Studies a U.S. FDA-regulated Device Product
No
Data Monitoring Committee
No
5. Study Description
Brief Summary
There is a growing body of evidence suggesting low water intake and elevated levels of the hormone vasopressin exacerbate glucose regulation. This project will examine the physiological mechanism by which low water intake impairs glucose homeostasis.
In the current proposal we aim to: 1) quantify the degree of glucose impairment as a response to elevated vasopressin due to low water intake and 2) identify the physiological mechanism by which elevated vasopressin, as a response to low water intake, impairs glucose regulation
6. Conditions and Keywords
Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
Glucose Metabolism Disorders, Dehydration (Physiology), Water Deprivation
Keywords
water, vasopressin
7. Study Design
Primary Purpose
Basic Science
Study Phase
Not Applicable
Interventional Study Model
Crossover Assignment
Masking
Participant
Allocation
Randomized
Enrollment
0 (Actual)
8. Arms, Groups, and Interventions
Arm Title
High Water intake
Arm Type
Active Comparator
Arm Description
5-days of High water intake according to IOM guidelines
Arm Title
Low Water Intake
Arm Type
Experimental
Arm Description
5-days of Low water intake of 500 mL/day
Intervention Type
Other
Intervention Name(s)
Water intake manipulation
Intervention Description
for 5 days prior to each experiment subjects will undergo manipulation of their water intake.
Primary Outcome Measure Information:
Title
amount of glucose metabolized M
Description
amount of glucose metabolized during the euglycemic & Hyperglycemic clamp
Time Frame
220-240 min & 340-360 min of the protocol
Title
metabolic clearance rate for insulin
Description
metabolic clearance rate for insulin during the euglycemic & Hyperglycemic clamp
Time Frame
220-240 min & 340-360 min of the protocol
Title
quantity of glucose metabolized per unit plasma insulin concentration
Description
quantity of glucose metabolized per unit plasma insulin concentration during the euglycemic & Hyperglycemic clamp
Time Frame
220-240 min & 340-360 min of the protocol
Title
Rate of glucose appearance & disappearance
Description
Rate of glucose appearance & disappearance during the euglycemic & Hyperglycemic clamp
Time Frame
220-240 min & 340-360 min of the protocol
Secondary Outcome Measure Information:
Title
Plasma copeptin
Description
Plasma copeptin in response to water manipulation
Time Frame
0 min, 240 min & 360 min of the protocol
Title
glucagon
Description
glucagon in response to water manipulation
Time Frame
0 min, 240 min & 360 min of the protocol
Title
Cortisol
Description
Cortisol in response to water manipulation
Time Frame
0 min, 240 min & 360 min of the protocol
10. Eligibility
Sex
All
Minimum Age & Unit of Time
30 Years
Maximum Age & Unit of Time
55 Years
Accepts Healthy Volunteers
Accepts Healthy Volunteers
Eligibility Criteria
Inclusion Criteria:
BMI 27.5-35
Exclusion Criteria:
BMI <27.5 or >35
high physical activity
diabetes
impaired liver or kidney function
cardiovascular disease
weight change of more than 3 kg in the past month
pregnancy
previous surgery on digestive tract (except appendectomy)
fluid balance will be exclusionary such as
serotonin re-uptake inhibitors.
12. IPD Sharing Statement
Plan to Share IPD
No
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The Effect of Water Intake on Glucose Regulation
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