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Tissue Sectioning by Electro-Dissociation

Primary Purpose

Thin Sections, Fresh Unfixed Tissues, Fresh Unfrozen Tissues

Status
Withdrawn
Phase
Locations
Study Type
Observational
Intervention
Tissue Sectioning via Electro Erosion Process
Sponsored by
University of Arkansas
About
Eligibility
Locations
Outcomes
Full info

About this trial

This is an observational trial for Thin Sections focused on measuring Histology, Immunohistochemical, Gene Expression, Tissue Sectioning

Eligibility Criteria

undefined - undefined (Child, Adult, Older Adult)All SexesDoes not accept healthy volunteers

Inclusion Criteria:

  • Fresh Tissue
  • Unfrozen Tissue
  • Unfixed Tissue

Exclusion Criteria:

  • Unfresh Tissue
  • Frozen Tissue
  • Fixed Tissue

Sites / Locations

    Outcomes

    Primary Outcome Measures

    Secondary Outcome Measures

    Full Information

    First Posted
    December 21, 2007
    Last Updated
    March 3, 2015
    Sponsor
    University of Arkansas
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    1. Study Identification

    Unique Protocol Identification Number
    NCT01054612
    Brief Title
    Tissue Sectioning by Electro-Dissociation
    Official Title
    Tissue Sectioning by Electro-Dissociation
    Study Type
    Observational

    2. Study Status

    Record Verification Date
    March 2015
    Overall Recruitment Status
    Withdrawn
    Why Stopped
    The study was closed due to the departure of several of the co-investigators.
    Study Start Date
    March 2002 (undefined)
    Primary Completion Date
    January 2010 (Actual)
    Study Completion Date
    January 2010 (Actual)

    3. Sponsor/Collaborators

    Name of the Sponsor
    University of Arkansas

    4. Oversight

    5. Study Description

    Brief Summary
    Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

    6. Conditions and Keywords

    Primary Disease or Condition Being Studied in the Trial, or the Focus of the Study
    Thin Sections, Fresh Unfixed Tissues, Fresh Unfrozen Tissues
    Keywords
    Histology, Immunohistochemical, Gene Expression, Tissue Sectioning

    7. Study Design

    Enrollment
    0 (Actual)

    8. Arms, Groups, and Interventions

    Intervention Type
    Other
    Intervention Name(s)
    Tissue Sectioning via Electro Erosion Process
    Intervention Description
    Focused RF energy can be used to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analysis by electro-dissociation without thermal damage, ultimately allowing high resolution reconstruction of gene and protein expression patterns of large tissue specimens in 3D.

    10. Eligibility

    Sex
    All
    Accepts Healthy Volunteers
    No
    Eligibility Criteria
    Inclusion Criteria: Fresh Tissue Unfrozen Tissue Unfixed Tissue Exclusion Criteria: Unfresh Tissue Frozen Tissue Fixed Tissue
    Study Population Description
    Discarded human tissue obtained immediately following surgical resection
    Sampling Method
    Probability Sample
    Overall Study Officials:
    First Name & Middle Initial & Last Name & Degree
    Gal Shafirstein, Ph.D.
    Organizational Affiliation
    UAMS, ACH, Central Arkansas Veterans Healthcare System
    Official's Role
    Principal Investigator

    12. IPD Sharing Statement

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    Tissue Sectioning by Electro-Dissociation

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