Mobilization and Handling of Stem Cells for Transplant From Healthy Volunteers With Sickle Cell Trait
Sickle Cell Trait
About this trial
This is an interventional treatment trial for Sickle Cell Trait focused on measuring Donor Apheresis, Sickle Cell Trait, Red Cell Alloimmunization
Eligibility Criteria
Confirmed diagnosis of sickle cell trait. Greater than age 17. No active systemic infection or history of recurrent infections requiring intravenous antibiotic treatment . Normal renal function: creatinine less than 2X normal, or proteinuria less than 1+. Normal liver function: bilirubin less than 2X normal, transaminases within normal limits. Normal blood count: WBC 3,000-10,000/mm3, granulocytes greater than 1,500/mm3, platelets greater than 150,000/mm3, hemoglobin greater than 12.5g/dL, MCV and MCHC within normal limits. Female volunteers of childbearing age should have a negative serum pregnancy test within one week of beginning G-CSF administration. Eligible for normal blood donation (i.e. tested negative for syphilis (RPR), hepatitis B and C (Hasbro, Anti-Hubcap, Anti-HCV), HIV, and HTLV-1. Subject must give informed consent to participate in the protocol. No history of sickle cell crises. Children less than 18 years of age are excluded as is per routine for normal volunteer protocols. No active systemic viral, bacterial, fungal, or parasitic infection. No female volunteers with positive pregnancy test or who are lactating. No history of autoimmune disease, such as rheumatoid arthritis and systemic lupus erythematosus. No history of cancer excluding squamous or basal cell carcinoma of the skin. No history of any malignant hematologic disorders. No history of cardiovascular disease or related symptoms such as chest pain and shortness of breath.. No allergy to G-CSF or bacterial E. coli products. No history of G-CSF administration or leukapheresis.
Sites / Locations
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)